Differential expression of PKCα and -β in the zebrafish retina
The retina is a complex neural circuit, which processes and transmits visual information from light perceiving photoreceptors to projecting retinal ganglion cells. Much of the computational power of the retina rests on signal integrating interneurons, such as bipolar cells. Commercially available antibodies against bovine and human conventional protein kinase C (PKC) α and -β are frequently used as markers for retinal ON-bipolar cells in different species, despite the fact that it is not known which bipolar cell subtype(s) they actually label. In zebrafish (Danio rerio) five prkc genes (coding for PKC proteins) have been identified. Their expression has not been systematically determined. While prkcg is not expressed in retinal tissue, the other four prkc (prkcaa, prkcab, prkcba, prkcbb) transcripts were found in different parts of the inner nuclear layer and some as well in the retinal ganglion cell layer. Immunohistochemical analysis in adult zebrafish retina using fluorescent in situ hybridization and PKC antibodies showed an overlapping immunolabeling of ON-bipolar cells that are most likely of the BON s6 and BON s6L or RRod type. However, comparison of transcript expression with immunolabeling, implies that these antibodies are not specific for one single zebrafish conventional PKC, but rather detect a combination of PKC -α and -β variants.
KeywordsZebrafish (RRID:ZIRC_ZL84) Protein kinase C Bipolar cell Retina
We would like to acknowledge Kara Kristiansen and Martin Walther for technical support and excellent fish care.
MFH carried out the experiments, participated in the design of the study and drafted and edited the manuscript; MB helped with the in situ hybridization and the fluorescent in situ hybridization, and drafted the manuscript; MG participated in the design of the study and edited the manuscript; SCFN participated in the design of the study, coordinated the study and edited the manuscript. All authors gave final approval for publication.
Compliance with ethical standards
Conflict of interest
The author declares that they have no conflict of interest.
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