Advertisement

Histochemistry and Cell Biology

, Volume 151, Issue 1, pp 21–28 | Cite as

Expression and localization of aromatase in human gastric mucosa

Immunohistochemical study using biopsy materials
  • Hiroto KobayashiEmail author
  • Saori Yoshida
  • Nobuyuki Shirasawa
  • Kunihiko Maeda
  • Akira Naito
Original Paper
  • 70 Downloads

Abstract

Parietal cells in the gastric mucosa are known not only as cells playing major roles in food digestion but also as cells bearing endocrine function. In addition to their production of gastrin and ghrelin, it has been recently revealed that these cells are also involved in the synthesis and secretion of estrogens with their expression of aromatase in experimental animals. Although aromatase activity has been detected in human gastric cancer cells and related cell lines, much less study has been done to ascertain the expression of the enzymatic activity in normal gastric mucosa. It has not been established which cell type is responsible for estrogen production in human gastric glands consisting of epithelial cells of several types. The aim of this study is to define the expression of aromatase by parietal cells in human gastric glands using immunohistochemical techniques. We retrieved formalin-fixed paraffin embedded materials of gastric biopsies from 16 patients (nine men, seven women). Colocalization of aromatase and H+/K+-ATPase β-subunit indicated that positive cells are parietal cells, but not chief cells and mucous cells. Furthermore, immunoreactivity of aromatase was detected within gastric glands irrespective of age or sex. These results suggest that human parietal cells synthesize estrogens within gastric mucosa and subsequently secrete them to the portal vein via gastric vein, as they do in rats. These estrogens might influence liver functions in humans. The estrogenic effects related to liver dysfunction might also be attributed to them.

Keywords

Human Immunohistochemistry Parietal cell Aromatase 17β-estradiol 

Notes

Acknowledgements

We are deeply grateful to laboratory members for giving us constructive comments and warm encouragement. Funding from the Annual Plan of Yamagata University (Grant Number DAY2609) is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest that would prejudice the impartiality of this research work.

Supplementary material

418_2018_1708_MOESM1_ESM.pdf (10.8 mb)
Supplementary material 1 (PDF 11090 KB)

References

  1. Dickson G (2012) Gynecomastia. Am Fam Physician 85:716–722Google Scholar
  2. Izawa M, Inoue M, Osaki M, Ito H, Harada T, Terakawa N, Ikeguchi M (2008) Cytochrome P450 aromatase gene (CYP19) expression in gastric cancer. Gastric Cancer 11:103–110CrossRefGoogle Scholar
  3. Kobayashi H, Yoshida S, Sun YJ, Shirasawa N, Naito A (2013a) Gastric estrogen increases pituitary estrogen receptor α and prolactin mRNAs during the different pathological conditions of the liver. Endocrine 43:170–183CrossRefGoogle Scholar
  4. Kobayashi H, Yoshida S, Sun YJ, Shirasawa N, Naito A (2013b) Postnatal development of gastric aromatase and portal venous estradiol-17β levels in male rats. J Endocrinol 218:117–124CrossRefGoogle Scholar
  5. Kobayashi H, Yoshida S, Sun YJ, Shirasawa N, Naito A (2013c) Gastric E2 and liver ERα correlate to serum E2 in the cholestatic male rat. J Endocrinol 219:39–49CrossRefGoogle Scholar
  6. Kobayashi H, Yoshida S, Sun YJ, Shirasawa N, Naito A (2014) Changes of gastric aromatase and portal venous 17β-estradiol during the postnatal development and estrus cycle in female rats. Endocrine 46:605–614CrossRefGoogle Scholar
  7. Kobayashi H, Yoshida S, Sun YJ, Shirasawa N, Naito A (2016a) 17β-Estradiol in the systemic circulation derives mainly from the parietal cells in cholestatic female rats. J Endocrinol Invest 39:389–400CrossRefGoogle Scholar
  8. Kobayashi H, Yoshida S, Sun YJ, Shirasawa N, Naito A (2016b) Gastric 17β-estradiol in portal vein and liver Esr1 make a circadian rhythm in systemic circulation in male rats. Endocrine 53:565–573CrossRefGoogle Scholar
  9. Nishi K, Tokunaga A, Shimizu Y, Yoshiyuki T, Wada M, Matsukura N, Tanaka N, Onda M, Asano G (1987) Immunohistochemical study of intracellular estradiol in human gastric cancer. Cancer 59:1328–1332CrossRefGoogle Scholar
  10. Saitoh Y, Sasano H, Naganuma H, Ohtani H, Sasano N, Ohuchi A, Matsuno S (1992) De novo expression of aromatase in gastric carcinoma. Light and electron microscopic immunohistochemical and immunoblot study. Res Pract 188:53–60CrossRefGoogle Scholar
  11. Ueyama T, Shirasawa N, Mtasuzawa M, Yamada K, Shelangouski M, Ito T, Tsuruo Y (2002) Gastric parietal cells: potent endocrine role in secreting estrogen as a possible regulator of gastro-hepatic axis. Endocrinology 143:3162–3170CrossRefGoogle Scholar
  12. Ueyama T, Shirasawa N, Ito T, Tsuruo Y (2004) Estrogen-producing steroidogenic pathways in parietal cells of the rat gastric mucosa. Life Sci 74:2327–2337CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hiroto Kobayashi
    • 1
    Email author
  • Saori Yoshida
    • 1
  • Nobuyuki Shirasawa
    • 2
  • Kunihiko Maeda
    • 3
  • Akira Naito
    • 1
  1. 1.Department of Anatomy and Structural Science, Faculty of MedicineYamagata UniversityYamagataJapan
  2. 2.Department of Rehabilitation, Faculty of Medical Science and WelfareTohoku Bunka Gakuen UniversitySendaiJapan
  3. 3.Yamagata Prefectural University of Health ScienceYamagataJapan

Personalised recommendations