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Intrascleral fixation of intraocular lens haptics: histological advantages in a comparison with scleral suture fixation in rabbits

  • Tsukasa HanemotoEmail author
  • Koichiro Mukai
  • Hiroyuki Matsushima
  • Tadashi Senoo
  • Tadahiko Kozawa
  • Yasuhiro Takahashi
  • Hirohiko Kakizaki
Basic Science
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Abstract

Purpose

To disclose histological advantages of intrascleral fixation of intraocular lens haptics, in comparison with scleral suture fixation, in a study with rabbits.

Methods

Ten white rabbits, 10 weeks of age, were used in this experimental histopathological study. After unilateral lensectomy and anterior vitrectomy, an intraocular lens haptic was inserted into one eye of each rabbit. Intrascleral fixation was performed in five rabbits, whereas scleral suture fixation was performed in the others. At postoperative 1 week, the globe was enucleated in two rabbits in each group; at postoperative 8 weeks, the globe was enucleated in the remaining three rabbits in each group. Sections of the sclera around haptics and sutures were evaluated with haematoxylin and eosin and immunohistochemical staining methods. We assessed severity of inflammation on histopathological photos, taken near the haptic or suture in the sclera, by counting white blood cells. The Mann-Whitney U test was performed to analyse differences in the severity of inflammation between the groups.

Results

Samples in the intrascleral fixation group demonstrated reduced irregularity of collagen fibres; reduced infiltration of fibroblasts, giant cells, lymphocytes, neovascular cells, neutrophils and eosinophils and weaker staining for fibronectin (indicating tissue repair) and heat shock protein 70 (indicating cell damage). In addition, reduced white blood cell infiltration was observed in the intrascleral fixation samples at 8 weeks in both shallow sclera (p = 0.001) and deep sclera (p = 0.002).

Conclusions

Histological analysis showed that intrascleral fixation caused fewer inflammatory changes than scleral suture fixation, with reduced fibroblast migration and production of cytotoxic factors.

Keywords

Intrascleral fixation Scleral suture fixation Histology Inflammation 

Notes

Acknowledgements

We thank Ryan Chastain-Gross, Ph.D., from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Tsukasa Hanemoto
    • 1
    • 2
    Email author
  • Koichiro Mukai
    • 1
  • Hiroyuki Matsushima
    • 1
  • Tadashi Senoo
    • 1
  • Tadahiko Kozawa
    • 2
  • Yasuhiro Takahashi
    • 3
  • Hirohiko Kakizaki
    • 3
  1. 1.Department of OphthalmologyDokkyo Medical UniversityTochigiJapan
  2. 2.Kozawa Eye HospitalMitoJapan
  3. 3.Department of Oculoplastic, Orbital and Lacrimal SurgeryAichi Medical University HospitalAichiJapan

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