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Forensic effectiveness and genetic distribution of 23 autosomal STRs included in Verifiler PlusTM multiplex in a population sample from Madhya Pradesh, India

  • Pankaj ShrivastavaEmail author
  • Kamlesh Kaitholia
  • R. K. Kumawat
  • Shivani Dixit
  • Hirak Ranjan Dash
  • Ankit Srivastava
  • Yash Baroniya
  • Aditi Mishra
  • Pushpesh Kushwaha
  • Manisha Rana
  • Akansha Dixit
  • Tanya Sarawagi
  • Alay Bhatt
  • Harsh Sharma
  • Moumita Sinha
  • Gyaneshwer Chaubey
Population Data

Abstract

We report here the first ever global study on genetic polymorphism using a Verifiler PlusTM autosomal STR multiplex system. The study evaluated genetic characteristics of 23 autosomal STRs in 200 unrelated residents of Guna district of Madhya Pradesh, India. Allele frequencies and forensic parameters are reported. Population comparison analysis was also performed using NJ tree and PCA plot. Penta E marker showed highest power of discrimination (0.938) among all 23 studied markers. The study also presents the first ever global forensic assessment in Indian population on D6S1043 marker (PD 0.937). The results demonstrated that all the 23 markers were highly polymorphic and the Verifiler PlusTM kit is suitable for forensic purposes in Indian population.

Keywords

Autosomal STR Forensic India Verifiler PlusTM Polymorphism 

Notes

Acknowledgments

The authors gratefully acknowledge the support of Director, State Forensic Science Laboratory, Sagar (MP), India, for providing laboratory facility as well as Thermo Fisher Scientific (India) for providing Verifiler PlusTM autosomal STR genotyping kit used in the study.

Compliance with ethical standards

The study was conducted in compliance with ethical standards. The study is approved by the ethical committee of Banaras Hindu University, Varanasi, India (Ref. No. I.Sc./ECM-XII/2018-19/06).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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References

  1. 1.
    Dixit, S. et al. (2019) Forensic genetic analysis of population of Madhya Pradesh with PowerPlex Fusion 6CTM Multiplex System. International journal of legal medicine 1–3.Google Scholar
  2. 2.
    Shrivastava P, Jain T, Trivedi VB (2015) Genetic polymorphism study at 15 autosomal locus in central Indian population. SpringerPlus 4:566–566CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Pankaj Shrivastava
    • 1
    Email author
  • Kamlesh Kaitholia
    • 1
  • R. K. Kumawat
    • 2
  • Shivani Dixit
    • 1
  • Hirak Ranjan Dash
    • 1
  • Ankit Srivastava
    • 3
  • Yash Baroniya
    • 3
  • Aditi Mishra
    • 1
  • Pushpesh Kushwaha
    • 1
    • 3
  • Manisha Rana
    • 1
    • 4
  • Akansha Dixit
    • 1
    • 3
  • Tanya Sarawagi
    • 1
  • Alay Bhatt
    • 1
  • Harsh Sharma
    • 1
  • Moumita Sinha
    • 4
  • Gyaneshwer Chaubey
    • 5
  1. 1.DNA Fingerprinting Unit, State Forensic Science Laboratory, Department of Home (Police)Govt. of MPSagarIndia
  2. 2.DNA Division, State Forensic Science Laboratory, RajasthanJaipurIndia
  3. 3.Institute of Forensic Science and CriminologyBundelkhand UniversityJhansiIndia
  4. 4.Department of Forensic ScienceGuru Ghasidas VishwavidyalayaBilaspurIndia
  5. 5.Cytogenetics Laboratory, Dept. of ZoologyBanaras Hindu UniversityVaranasiIndia

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