Environmental factors influencing flight activity of forensically important female blow flies in Central Europe
In forensic entomology, evaluation of a possible delay between a person’s death and insect colonization is crucial. We monitored the seasonal flight activities of the most abundant blow flies in an urban habitat in Frankfurt/Germany based on 152 sampling days between April and October 2017. Thirty-six thousand female specimens of 12 necrophagous taxa were sampled as a possible groundwork for establishing a prediction tool for the activity of certain forensically relevant taxa. The most abundant taxon was Lucilia sericata (n = 19,544), followed by Lucilia caesar (n = 8025), Calliphora vicina (n = 5224), and Lucilia ampullacea (n = 1834). Up to six environmental parameters were statistically significant predictors of fly presence, leading to unique patterns of seasonal and daily activity for all four species. In detail, our analysis proved that L. sericata is a sun-loving, high-summer species that dominates the warmer months and is mostly influenced by mean day temperature. In contrast, L. caesar seems to be a shade-loving species that dominates in autumn resp. late-season and is mainly influenced by mean day temperature and wind speed. The activity of L. ampullacea was highly related to mean day temperature and relative humidity. In contrast to all other species, C. vicina behaved differently, particularly due to its occurrence throughout the entire sampling interval and the higher tolerance limits for the measured abiotic parameters, especially temperature. The present study is groundwork for establishing a prediction tool for the flight and oviposition activity of forensically relevant taxa.
KeywordsFemale blowflies Forensic entomology Abiotic parameters Monitoring
The authors would like to thank D. Ricciardi and V. Bernhardt for their assistance during fieldwork, an anonymous colleague for helping with the statistical analysis, and the two anonymous reviewers for their careful reading and commenting of the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Villet MH, Richards CS, Midgley JM (2010) Contemporary precision, bias and accuracy of minimum post-mortem intervals estimated using development of carrion-feeding insects. In: Campobasso G (ed) Amendt J, Goff M. Current Concepts in Forensic Entomology. Springer, Dordrecht, pp 109–137Google Scholar
- 5.Smith KGV (1986) A manual of forensic entomology. British Museum, LondonGoogle Scholar
- 8.Tomberlin JK, Mohr R, Benbow ME, Tarone AM, VanLaerhoven S (2011) A roadmap for bridging basic and applied research in forensic entomology. Annu Rev Entomol 56:401–421. https://doi.org/10.1146/annurev-ento-051710-103143 CrossRefGoogle Scholar
- 31.Digby PSB (1958) Flight activity in the blowfly, Calliphora erythrocephala, in relation to wind speed, with special reference to adaptation. J Exp Biol 35:776–795Google Scholar
- 35.Smith JL, Palermo NA, Theobald JC, Wells JD (2016) The forensically important blow fly, Chrysomya megacephala (Diptera: Calliphoridae), is more likely to walk than fly to carrion at low light levels. Forensic Sci Int 266:245–249. https://doi.org/10.1016/j.forsciint.2016.06.004 CrossRefGoogle Scholar
- 36.Erzinçlioğlu Z (1996) Blowflies. Richmond Publishing, SloughGoogle Scholar
- 40.Rognes K (1991) Blowflies (Diptera, Calliphoridae) of Fennoscandia and Denmark. Scandinavian Science Press, LeidenGoogle Scholar
- 41.Szpila K (2012) Key for identification of European and Mediterranean blowflies (Diptera, Calliphoridae) of medical and veterinary importance - adult flies. In: Gennard (ed) Forensic entomology: an introduction, 2nd edn. Wiley-Blackwell, Hoboken, pp 77–81Google Scholar
- 46.Pohjoismäki JLO, Karhunen PJ, Goebeler S, Saukko P, Sääksjärvi IE (2010) Indoors forensic entomology: colonization of human remains in closed environments by specific species of sarcosaprophagous flies. Forensic Sci Int 199:38–42. https://doi.org/10.1016/j.forsciint.2010.02.033 CrossRefGoogle Scholar
- 50.Barry RG, Chorely RJ (2003) Atmosphere, weather and climate. Routledge, LondonGoogle Scholar
- 58.Smith KE, Wall R (1997) The use of carrion as breeding sites by the blowfly Lucilia sericata and other Calliphoridae. Med Vet Entomol 11:38–44. https://doi.org/10.1111/j.1365-2915.1997.tb00287.x CrossRefGoogle Scholar
- 62.Kuusela S, Hanski I (1982) The structure of carrion fly communities: the size and the type of carrion. Ecogr 5:337–348. https://doi.org/10.1111/j.1600-0587.1982.tb01048.x CrossRefGoogle Scholar
- 66.Gennard D (2012) Forensic entomology: an introduction. Wiley-Blackwell, HobokenGoogle Scholar