Population genetic analysis of 30 insertion–deletion (INDEL) loci in a Qinghai Tibetan group using the Investigator DIPplex Kit
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The Qiagen Investigator® DIPplex Kit multiplexes 30 autosomal INDELs plus amelogenin for forensic use. The aim of this study was to estimate the diversity of 30 INDEL loci in a sample of 530 Tibetan individuals from Qinghai Province, China, and to evaluate the usefulness of these loci for forensic genetics. The observed heterozygosity ranged from 14.3 to 53.4%, and combined power of discrimination, matching probability, and cumulative probability of exclusion were 0.99999999999172, 8.27999 × 10−12, and 0.9897, respectively, in the Qinghai Tibetan group. The results of pairwise genetic distance, principal component analysis, a multi-dimensional scaling plot, and a neighbour-joining tree between the Qinghai Tibetan and 49 reference populations revealed significant genetic differences between continental populations and a close genetic relationship between the Qinghai Tibetan and East Asian populations. This study indicates that the Investigator® DIPplex Kit can serve as an effective supplementary tool for forensic genetic tasks.
KeywordsInvestigator® DIPplex kit INDEL Qinghai Tibetan Forensic genetics Population study
We thank all sample donors for their contributions to this work and all those who helped with sample collection.
This work received financial support from the Department of Science & Technology of Sichuan Province (2017JY0233).
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Conflict of interest
The authors declare that they have no conflict of interest.
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