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International Journal of Legal Medicine

, Volume 133, Issue 4, pp 1039–1041 | Cite as

Population genetic analysis of 30 insertion–deletion (INDEL) loci in a Qinghai Tibetan group using the Investigator DIPplex Kit

  • Hui Jian
  • Li Wang
  • Hui Wang
  • Xiaogang Bai
  • Meili LvEmail author
  • Weibo LiangEmail author
Population Data

Abstract

The Qiagen Investigator® DIPplex Kit multiplexes 30 autosomal INDELs plus amelogenin for forensic use. The aim of this study was to estimate the diversity of 30 INDEL loci in a sample of 530 Tibetan individuals from Qinghai Province, China, and to evaluate the usefulness of these loci for forensic genetics. The observed heterozygosity ranged from 14.3 to 53.4%, and combined power of discrimination, matching probability, and cumulative probability of exclusion were 0.99999999999172, 8.27999 × 10−12, and 0.9897, respectively, in the Qinghai Tibetan group. The results of pairwise genetic distance, principal component analysis, a multi-dimensional scaling plot, and a neighbour-joining tree between the Qinghai Tibetan and 49 reference populations revealed significant genetic differences between continental populations and a close genetic relationship between the Qinghai Tibetan and East Asian populations. This study indicates that the Investigator® DIPplex Kit can serve as an effective supplementary tool for forensic genetic tasks.

Keywords

Investigator® DIPplex kit INDEL Qinghai Tibetan Forensic genetics Population study 

Notes

Acknowledgments

We thank all sample donors for their contributions to this work and all those who helped with sample collection.

Funding information

This work received financial support from the Department of Science & Technology of Sichuan Province (2017JY0233).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Forensic Genetics, West China School of Basic Science and Forensic MedicineSichuan UniversityChengduChina
  2. 2.Department of Obstetrics and Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University) of Ministry of EducationWest China Second University Hospital, Sichuan UniversityChengduChina
  3. 3.Chengdu Public Security BureauInstitute of Forensic ScienceChengduChina
  4. 4.Department of Immunology, West China School of Preclinical and Forensic MedicineSichuan UniversityChengduChina

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