International Journal of Legal Medicine

, Volume 133, Issue 2, pp 571–581 | Cite as

Post-mortem thermal angiography: a pilot study on swine coronary circulation

  • Paolo Fais
  • Maria Carla Mazzotti
  • Massimo Montisci
  • Chiara Palazzo
  • Ornella Leone
  • Giovanni Cecchetto
  • Guido VielEmail author
  • Susi Pelotti
Method Paper


Thermal imaging (TI) allows the detection of thermal patterns emitted from objects as a function of their temperature in the long-infrared spectrum and produces visible images displaying temperature differences. The aim of this pilot study was to test TI to visualize the coronary circulation of swine hearts. Thirty swine hearts were prepared for ex situ coronarography, and thermal images were acquired through a FlirOne thermal camera (FLIR Systems®) paired with a Google Android Smartphone. Coronary arteries were cannulated, namely the anterior interventricular artery, the circumflex branch of the left coronary artery, and the right coronary artery. The heart was cooled, and contrast medium (CM) consisting of distilled water heated to 40 °C was injected in a coronary vessel, while thermal images were captured. These steps were repeated for each coronary vessel and under experimentally simulated coronary heart disease. Thermal imaging coronarography (TIC) allowed a clear representation of the morphology and course of the coronary vessels and of experimentally simulated coronary heart disease, moreover, demonstrated to be easy to perform during or after autopsies on ex situ hearts, non-destructive, reproducible, and cheap. On the basis of these preliminary results, TIC might allow a subsequent more focused and comprehensive cardiopathological examination of the heart, which remains mandatory for the definitive diagnosis of coronary heart disease. Although these preliminary results seem encouraging, further systematic studies on human hearts, both normal and pathological, are necessary for estimating the sensitivity and specificity of the proposed method and to draw any definitive conclusion.


Thermal imaging Post-mortem coronarography Cardiopathology Post-mortem imaging Ex situ coronarography 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Ammer K, Ring EFJ (2005) Influence of the field of view on temperature readings from thermal images. Thermol Int 15:99–103Google Scholar
  2. 2.
    Kateb B, Yamamoto V, Yu C, Grundfest W, Gruen JP (2009) Infrared thermal imaging: a review of the literature and case report. Neuroimage 47:154–162. CrossRefGoogle Scholar
  3. 3.
    Love TJ (1980) Thermography as an indicator of blood perfusion. Ann N Y Acad Sci 335:429–437CrossRefGoogle Scholar
  4. 4.
    Merla A, Romani GL (2006) Functional infrared imaging in clinical applications. In: Bronzino JD (ed) The biomedical engineering handbook. CRC Press, USA, pp 32.1–32.16Google Scholar
  5. 5.
    Keyserlingk JR, Ahlgren PD, Yu E, Belliveau N, Yassa M (2000) Functional infrared imaging of the breast. IEEE Eng Med Biol Mag 19:30–41CrossRefGoogle Scholar
  6. 6.
    Head JF, Lipari CA, Elliott RL (1999) Comparison of mammography and breast infrared imaging: sensitivity, specificity, false negatives, false positives, positive predictive value and negative predictive value. 21st Ann Int Conf IEEE Eng Med Biol Soc.
  7. 7.
    Gautherie M, Gros CM (1980) Breast thermography and cancer risk prediction. Cancer 45:51–56CrossRefGoogle Scholar
  8. 8.
    Stark AM (1985) The value of risk factors in screening for breast cancer. Eur J Surg Oncol 11:147–150Google Scholar
  9. 9.
    Cole RP, Jones SG, Shakespeare PG (1990) Thermographic assessment of hand burns. Burns 16:60–63CrossRefGoogle Scholar
  10. 10.
    Hargroder AG, Davidson JE, Luther DG, Head JF (1999) Infrared imaging of burn wounds to determine burn depth. Infrared Tech Applic 3698:103–108. CrossRefGoogle Scholar
  11. 11.
    Esselinckx W, Bacon PA, Ring EF, Crooke D, Collins AJ, Demottaz D (1978) A thermographic assessment of three intra-articular prednisolone analogues given in rheumatoid synovitis. Br J Clin Pharmacol 5:447–451CrossRefGoogle Scholar
  12. 12.
    Bird HA, Ring EF, Bacon PA (1979) A thermographic and clinical comparison of three intra-articular steroid preparations in rheumatoid arthritis. Ann Rheum Dis 38:36–39CrossRefGoogle Scholar
  13. 13.
    Bacon PA, Ring EFJ, Collins AJ (1977) Thermography in the assessment of anti rheumatic agents. In: Gordon JL, Hazleman BL (eds) Rheumatoid arthritis. Elsevier/North Holland Biomedical Press, Amsterdam, p 105Google Scholar
  14. 14.
    Fischer AA, Chang CH (1986) Temperature and pressure threshold measurements in trigger points. Thermology 1:212–215Google Scholar
  15. 15.
    Schmitt M, Guillot Y (1984) Thermography and muscular injuries in sports medicine. In: Ring EFJ, Phillips B (eds) Recent advances in medical thermology. Springer, Boston, pp 439–445CrossRefGoogle Scholar
  16. 16.
    Ng EYK, Kawb GJL, Chang WM (2004) Analysis of IR thermal imager for mass blind fever screening. Microvasc Res 68:104–109CrossRefGoogle Scholar
  17. 17.
    Ng EY (2005) Is thermal scanner losing its bite in mass screening of fever due to SARS? Med Phys 32:93–97CrossRefGoogle Scholar
  18. 18.
    Chan LS, Cheung GT, Lauder IJ, Kumana CR, Lauder IJ (2004) Screening for fever by remote-sensing infrared thermographic camera. J Travel Med 11:273–279CrossRefGoogle Scholar
  19. 19.
    Li WW, Head JF (2000) Infrared imaging in the detection and evaluation of tumor angiogenesis. 22nd Ann Int Conf IEEE Eng Med Biol Soc.
  20. 20.
    Szabò Z, Berg S, Sjokvist S, Gustafsson T, Carleberg P, Uppsall M, Wren J, Ahn H, Smedby O (2013) Real-time intraoperative visualization of myocardial circulation using augmented reality temperature display. Int J Card Imaging 29:521–528CrossRefGoogle Scholar
  21. 21.
    Mall G, Hubig M, Beier G, Buttner A, Eisenmenger W (2002) Supravital energy production in early post-mortem phase—estimate based on heat loss due to radiation and natural convection. Legal Med 4:71–78CrossRefGoogle Scholar
  22. 22.
    Johnson AP, Mikac KM, Wallman JF (2013) Thermogenesis in decomposing carcasses. Forensic Sci Int 231:271–277. CrossRefGoogle Scholar
  23. 23.
    Desmarais AM (2014) Detection of cadaveric remains by thermal imaging cameras. J Forensic Ident 64:489–510Google Scholar
  24. 24.
    Amendt J, Rodner S, Schuch CP, Sprenger H, Weidlich L, Reckel F (2017) Helicopter thermal imaging for detecting insect infested cadavers. Sci Justice 57:366–372. CrossRefGoogle Scholar
  25. 25.
    Bruguier C, Egger C, Vallèe JP, Grimm J, Boulanger X, Jackowski C, Mangin P, Grabherr S (2015) Postmortem magnetic resonance imaging of the heart ex situ: development of technical protocols. Int J Legal Med 129:559–567CrossRefGoogle Scholar
  26. 26.
    Basso C, Aguilera B, Banner J et al (2017) Guidelines for autopsy investigation of sudden cardiac death: 2017 update from the Association for European Cardiovascular Pathology. Virchows Arch 471:691–705CrossRefGoogle Scholar
  27. 27.
    Dyce K, Sack W, Wensing CJG (2010) Textbook of veterinary anatomy. Saunders-Elsevier, St. Louis, pp 233–234Google Scholar
  28. 28.
    Weaver ME, Pantely GA, Bristow JD, Ladley HD (1986) A quantitative study of the anatomy and distribution of coronary arteries in swine in comparison with other animals and man. Cardiovasc Res 20:907–917CrossRefGoogle Scholar
  29. 29.
    Mokrane FZ, Dercle L, Meyrignac O, Crubezy E, Rousseau H, Telmon N, Dedouit F (2018) Towards multi-phase postmortem CT angiography in children: a study on a porcine model. Int J Legal Med 132:1391–1403. CrossRefGoogle Scholar
  30. 30.
    Goldstein S (1982) The necessity of a uniform definition of sudden coronary death: witnessed death within 1 hour of the onset of acute symptoms. Am Heart J 103:156–159CrossRefGoogle Scholar
  31. 31.
    Michaud K, Grabherr S, Doenz F, Mangin P (2012) Evaluation of postmortem MDCT and MDCT-angiography for the investigation of sudden cardiac death related to atherosclerotic coronary artery disease. Int J Card Imaging 28:1807–1822CrossRefGoogle Scholar
  32. 32.
    Palmiere C, Lobrinus JA, Mangin P, Grabherr S (2013) Detection of coronary thrombosis after multi-phase postmortem CT-angiography. Leg Med (Tokyo) 15:12–18CrossRefGoogle Scholar
  33. 33.
    Jackowski C, Schwendener N, Grabherr S, Persson A (2013) Post-mortem cardiac 3-T magnetic resonance imaging: visualization of sudden cardiac death? J Am Coll Cardiol 62:617–629CrossRefGoogle Scholar
  34. 34.
    Michaud K, Grabherr S, Jackowski C, Bollmann MD, Doenz F, Mangin P (2014) Postmortem imaging of sudden cardiac death. Int J Legal Med 128:127–137CrossRefGoogle Scholar
  35. 35.
    Michaud K, Grabherr S, Faouzi M, Grimm J, Doenz F, Mangin P (2015) Pathomorphological and CT-angiographical characteristics of coronary atherosclerotic plaques in cases of sudden cardiac death. Int J Legal Med 129:1067–1077CrossRefGoogle Scholar
  36. 36.
    Sabatasso S, Vanhaebost J, Doenz F, Palmiere C, Michaud K, Dedouit F, Grabherr S (2018) Visualization of myocardial infarction in postmortem multiphase computed tomography angiography: a feasibility study. Am J Forensic Med Pathol 39:106–113CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.DIMEC, Department of Medical and Surgical SciencesUniversity of BolognaBolognaItaly
  2. 2.Legal Medicine and ToxicologyUniversity Hospital of PadovaPadovaItaly
  3. 3.Department of PathologySant’Orsola-Malpighi University HospitalBolognaItaly

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