Population data and phylogenetic structure of Han population from Jiangsu province of China on GlobalFiler STR loci
- 163 Downloads
Forensic statistical parameters based on allelic frequencies of commonly used short tandem repeats were estimated for the Han population of Jiangsu province from P.R. China. The 6-dye GlobalFiler™ PCR amplification kit incorporates 21 autosomal STRs, providing reliable DNA typing results with enhanced the power of discrimination. Here, we analyzed the GlobalFiler™ STR loci in 516 unrelated individuals from Jiangsu Han population. A total of 256 alleles were observed ranging between 5 and 35.2 repeat units, and SE33 showed the greatest power of discrimination (34 alleles) in Jiangsu Han population. Most of the loci were found to be in the Hardy-Weinberg equilibrium after the Bonferroni correction with the exception of D3S1358. The combined power of exclusion (CPE) was 0.999999996353609, and the combined match probability (CMP) was 3.64 × 10−25. Phylogenetic parameters including pairwise genetic distances showed that Han population living in Jiangsu had closest genetic relationship with other East Asian populations. The present study provides precise reference database for forensic applications and population genetic studies.
KeywordsForensic population database Jiangsu Han China GlobalFiler™
This project is supported by the National Natural Science Foundation of P.R. China (NSFC, No. 81471826), Ministry of Finance, P.R. China.
Compliance with ethical standards
All participants gave their informed consent in writing after the study aims and procedures were carefully explained to them in their own language. The study was approved by the ethical review board of the China Medical University, Shenyang Liaoning Province, People’s Republic of China, and in accordance with the standards of the Declaration of Helsinki.
Conflict of interest
The authors declare that they have no conflict of interest.
- 4.Wang DY, Gopinath S, Lagacé RE, Norona W, Hennessy LK, Short ML, Mulero JJ (2015) Developmental validation of the GlobalFiler(®) express PCR amplification kit: a 6-dye multiplex assay for the direct amplification of reference samples. Forensic Sci Int Genet 19:148–155. https://doi.org/10.1016/j.fsigen.2015.07.013 CrossRefPubMedGoogle Scholar
- 6.Bi R, Zhang W, Yu D, Li X, Wang HZ, Hu QX, Zhang C, Lu W, Ni J, Fang Y, Li T, Yao YG (2015) Mitochondrial DNA haplogroup B5 confers genetic susceptibility to Alzheimer’s disease in Han Chinese. Neurobiol Aging 36:1604.e7–1604.16. https://doi.org/10.1016/j.neurobiolaging.2014.10.009 CrossRefGoogle Scholar
- 9.Bär W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B (1997) DNA recommendations. Further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. International Society for Forensic Haemogenetics. Int J Legal Med 110:175–176CrossRefPubMedGoogle Scholar
- 10.Gusmão L, Butler JM, Carracedo A, Gill P, Kayser M, Mayr WR, Morling N, Prinz M, Roewer L, Tyler-Smith C, Schneider PM, DNA Commission of the International Society of Forensic Genetics (2006) DNA Commission of the International Society of Forensic Genetics (ISFG): an update of the recommendations on the use of Y-STRs in forensic analysis. Forensic Sci Int 157:187–197. https://doi.org/10.1016/j.forsciint.2005.04.002 CrossRefPubMedGoogle Scholar
- 11.Bodner M, Bastisch I, Butler JM, Fimmers R, Gill P, Gusmão L, Morling N, Phillips C, Prinz M, Schneider PM, Parson W (2016) Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on quality control of autosomal Short Tandem Repeat allele frequency databasing (STRidER). Forensic Sci Int Genet 24:97–102. https://doi.org/10.1016/j.fsigen.2016.06.008 CrossRefPubMedGoogle Scholar
- 14.Park H-C, Kim K, Nam Y, Park J, Lee J, Lee H, Kwon H, Jin H, Kim W, Kim W, Lim S (2016) Population genetic study for 24 STR loci and Y indel (GlobalFiler™ PCR Amplification kit and PowerPlex® Fusion system) in 1000 Korean individuals. Legal Med 21:53–57. https://doi.org/10.1016/j.legalmed.2016.06.003 CrossRefPubMedGoogle Scholar
- 15.Fujii K, Watahiki H, Mita Y, Iwashima Y, Kitayama T, Nakahara H, Mizuno N, Sekiguchi K (2015) Allele frequencies for 21 autosomal short tandem repeat loci obtained using GlobalFiler in a sample of 1501 individuals from the Japanese population. Legal Med 17:306–308. https://doi.org/10.1016/j.legalmed.2015.08.007 CrossRefPubMedGoogle Scholar
- 17.Alsafiah HM, Goodwin WH, Hadi S, Alshaikhi MA, Wepeba PP (2017) Population genetic data for 21 autosomal STR loci for the Saudi Arabian population using the GlobalFiler ® PCR amplification kit. Forensic Sci Int Genet 31:e59–e61. https://doi.org/10.1016/j.fsigen.2017.09.014 PubMedCrossRefGoogle Scholar
- 18.Zhang H, Yang S, Guo W, Ren B, Pu L, Ma T, Xia M, Jin L, Li L, Li S (2016) Population genetic analysis of the GlobalFiler STR loci in 748 individuals from the Kazakh population of Xinjiang in northwest China. Int J Legal Med 130:1187–1189. https://doi.org/10.1007/s00414-016-1319-2 CrossRefPubMedGoogle Scholar
- 19.Zhang H, Xia M, Qi L, Dong L, Song S, Ma T, Yang S, Jin L, Li L, Li S (2016) Forensic and population genetic analysis of Xinjiang Uyghur population on 21 short tandem repeat loci of 6-dye GlobalFiler™ PCR Amplification kit. Forensic Sci Int Genet 22:22–24. https://doi.org/10.1016/j.fsigen.2016.01.005 CrossRefPubMedGoogle Scholar
- 20.Felsenstein J (2009) PHYLIP (phylogeny inference package) Version 3.69. Department of Genome Sciences, University of Washington, SeattleGoogle Scholar