Detection of high-risk human papillomavirus in the tonsils of galilee region adults and young adults undergoing tonsillectomy
The presence of high-risk HPV in non-malignant tonsil tissues from patients who underwent tonsillectomy in the Galilee area might explain the low incidence of HPV-related oropharyngeal malignancy in the country. The aim of this retrospective study was to study the prevalence of human papillomavirus (HPV) in non-malignant tonsillectomy specimens of adults in the Galilee area.
Materials and methods
We conducted a retrospective analysis of all tonsil samples in our medical center. Tonsils from patients over 20 years of age who underwent tonsillectomy for the indication of recurrent tonsillitis and sleep apneas were eligible. Samples of formalin-fixed paraffin-embedded (FFPE) tonsillar tissue were tested for the presence of HPV DNA using polymerase chain reaction and by p16 immunohistochemistry.
Of the 71 tonsil samples, age range 20–65, none were positive for HPV DNA. Fifty-two FFPE specimens of tonsil with HPV-positive cancer tested positive by the same method served as positive controls.
HPV DNA is rare in non-malignant tonsil tissues of young adults and adults who underwent tonsillectomy in the Galilee area. Further research should be done in larger cohorts.
KeywordsHuman papillomavirus Tonsillectomy Prevalence
Squamous cell carcinomas of the head and neck
Oropharyngeal squamous cell carcinoma
Polymerase chain reaction
We wish to thank Mrs. Orly Yakir was responsible for the statistical analysis. We wish to thank Migal-cbr for the proof-read.
Compliance with ethical standards
Conflict of interest
Reut Aviv Mordechai, Shay Steinberg, Liat Apel-Sarid, Ety Shaoul, Simona Rozen, Lubna Khoury, and Ohad Ronen declare that they have no conflict of interest.
Ethical approval and informed consent
The study was approved by the institutional review board prior to its commencement (Registration number: NHR010714). Written consent was waived by the institutional review board.
- 4.Elrefaey S, Massaro MA, Chiocca S, Chiesa F, Ansarin M (2014) HPV in oropharyngeal cancer: the basics to know in clinical practice. Acta Otorhinolaryngol Ital 34(5):299–309Google Scholar
- 15.Kreimer AR, Bhatia RK, Messeguer AL, Gonzalez P, Herrero R, Giuliano AR (2010) Oral human papillomavirus in healthy individuals: a systematic review of the literature. Sex Transm Dis 37(6):386–391Google Scholar
- 18.Wilson R, Paterson Pa, Larson HJ (2014) The HPV VAccination in Japan, issues and options. center for strategic and international studies. https://www.rho.org/files/CSIS_HPV_vac_issues_options_Japan_2014.pdf. Accessed 30 Mar 2019
- 23.Bruni L, Albero G, Serrano B et al (2019) Human papillomavirus and related diseases in the World. Summary report. ICO/IARC Information Centre on HPV and Cancer. https://www.hpvcentre.net/statistics/reports/XWX.pdf. Accessed 30 Mar 2019
- 26.Human papillomavirus vaccines: WHO position paper, October 2014 Releve epidemiologique hebdomadaire. https://www.who.int/wer/2014/wer8943.pdf. Accessed 9 July 2019
- 28.Ferlay J, Ervik M, Lam F et al (2018) No TitleCancer incidence and mortality worldwide: IARC CancerBase no. 11. GLOBOCAN. https://publications.iarc.fr/577. Accessed 30 Mar 2019
- 32.Sun Y, Wu Y, Wu L et al (2012) Detection of human papillomavirus in the upper respiratory tract in children without recurrent respiratory papillomatosis. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 47(12):974–977Google Scholar
- 33.Baloğlu H, Küçükodaci Z, Gungor A et al (2010) Human papilloma virus prevalence in hyperplastic tonsils and adenoids in children and young adults. Turk Klin J Med Sci 30:528–532Google Scholar
- 34.Soldatskii IA, Onufrieva EK, Pogosova IE, Strygina IV, Diudia AV (2009) Infection of the upper respiratory tract with human papilloma virus in children without clinical signs of respiratory papillomatosis. Vestn Otorinolaringol 1:16–19Google Scholar