European Archives of Oto-Rhino-Laryngology

, Volume 276, Issue 5, pp 1321–1325 | Cite as

Association between occurrence of ossicular chain defect and osteoprotegerin gene expression in patients with chronic otitis media

  • Serhan Keskin
  • Arzu TatlıpınarEmail author
  • Pınar Ata
  • Selami Uzun
  • Mustafa Emrah Kınal
  • Burçin Erkal



Chronic otitis media (COM) is an important debilitating public problem causing hearing loss due to irreversible resorption of the ossicular chain. Activation of osteoprotegerin (OPG) during an acute attack of COM prevents bone resorption.The aim of the study was to investigate the role of OPG gene expression level on ossicular chain resorption in chronic otitis media.

Materials and methods

Fifty operated COM patients were included in the study. While 20 patients underwent ossiculoplasty, 30 patients underwent type 1 tympanoplasty. For RNA isolation and OPG gene expression analysis, middle ear swabs were taken from nasopharynx in the ostium of the Eustachian tube. RNA was isolated with mRNA easy kit and kept at − 85 °C till the cDNA and expression analysis. Expression levels were analyzed with real-time quantitative PCR in comparison with PDGB gene expression level as an internal control.


Sample Cq measurements of type 1 tympanoplasty group were higher than Cq measurements of the internal control group (p = 0.027; p < 0.05). In contrast, there was no statistically significant difference between sample Cq measurements of ossiculoplasty group and Cq measurements of the internal control group (p = 0.293; p > 0.05).


Since OPG gene expression level was significantly higher in type 1 tympanoplasty group, OPG gene regulation system may have an effect on ossicular chain destruction in COM.


Tympanoplasty Ossiculoplasty Osteoprotegerin Gene expression 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Serhan Keskin
    • 1
  • Arzu Tatlıpınar
    • 1
    Email author
  • Pınar Ata
    • 2
  • Selami Uzun
    • 1
  • Mustafa Emrah Kınal
    • 1
  • Burçin Erkal
    • 2
  1. 1.Ear Nose and Throat ClinicHealth Science University Fatih Sultan Mehmet Health Practice and Research CenterIstanbulTurkey
  2. 2.Department of Medical GeneticMarmara University School of MedicineIstanbulTurkey

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