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Differential expression of immune markers in the patients with obstructive sleep apnea/hypopnea syndrome

  • Hong Xie
  • Jinshu YinEmail author
  • Yunbo Bai
  • Hong Peng
  • Xiaohong Zhou
  • Juan Bai
Rhinology

Abstract

Purpose

To evaluate phenotypic changes of various immune cells in the peripheral blood in the patients with sleep apnea/hypopnea syndrome (OSAHS).

Methods

This is a case–control study. The peripheral venous blood was collected. A subset of T cells, B cells, natural killer cells, and dendritic cells was analysed using various markers and flow cytometry. Regression curve analysis was made to examine the correlation between the change of immune cells and aponea hypoxia index (AHI) and oxygen desaturation.

Results

The percentage of CD3+/CD4+ T lymphocytes (P < 0.001) and CD19+ B cells (P < 0.001) and the CD4+/CD8+ ratio (P < 0.001) in the OSAHS patients were significantly increased compared with those in the control group without OSAHS, and CD4+/CD8+ ratio positively correlated with aponea hypoxia index (r = 0.37, P < 0.001) but negatively correlated with the lowest SaO2 (r = − 0.2, P < 0.001), whereas a greater reduction in the percentage of CD3+/CD8+ T cells (P < 0.001). Moreover, the ratios of CD3+/CD16+/CD56+ natural killer (NK)-like T cells (P < 0.05) and CD3/CD16+/CD56+ NKT cells (P < 0.001) were significantly lower in the OSAHS group than those in the control group. However, no significant difference was observed in the percentage of CD3+ total T cells, CD8+/CD28+ T cells, CD8+/CD28 T cells, DC1, DC2, and DC1/DC2 dendritic cells between the OSAHS and control groups.

Conclusion

Our study showed differential responses of various types of immune cells in the peripheral blood in patients with OSAHS and their correlation with severity of oxygen desaturation.

Keywords

T cells B cells Natural killer cells Dendritic cells Flow cytometry Obstructive sleep aponea 

Notes

Compliance with ethical standards

Conflict of interest

Hong Xie has no conflict of interest, Jinshu Yin has no conflict of interest, Yunbo Bai has no conflict of interest, Hong Peng has no conflict of interest, Xiaohong Zhou has no conflict of interest, and Juan Bai has no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

All participants were informed about the study and signed an informed consent form. This study was approved by the Ethical Committee of Beijing Shijitan Hospital.

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© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Otorhinolaryngology Head and Neck Surgery, Beijing Shijitan HospitalCapital Medical UniversityBeijingChina

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