European Archives of Oto-Rhino-Laryngology

, Volume 275, Issue 12, pp 2957–2966 | Cite as

The protective effect of metformin against the noise-induced hearing loss

  • Gülin Gökçen KesiciEmail author
  • Fatma Ceyda Akın Öcal
  • Seren Gülşen Gürgen
  • Şaban Remzi Erdem
  • Ersin Öğüş
  • Hatice Seyra Erbek
  • Levent Naci Özlüoğlu



To test the protective effect of metformin against noise-induced hearing loss.


24 rats were included in the study. The first group was exposed to noise only, the second group took metformin, the third group was exposed to noise and took metformin, and the fourth group was neither exposed to noise nor took metformin as control group. After measurement of baseline DPOAE and ABR of rats, the metformin group and the metformin + noise group received 300 mg/kg/day metformin via gavage for 10 days. On the 11th day, group 1 and group 3 were exposured to white noise at 105 dB SPL for 15 h. After noise exposure, DPOAE and ABR measurements of all rats were repeated on days 1st, 7th, and 21st. At the end of the study, all animals were sacrificed and cochlear tissues were separated for immunohistochemical assessments.


ABR threshold values and DPAOE measurements of groups 1 and 3 were deteriorated on the 1st day after noise, while deterioration in group 1 continued on 7th and 21st days, but normalized on 7th day in group 3. After immune staining, a significant immunoreaction was observed in the noise group, while the reaction in the noise + metformin group was close to the control group.


Metformin has a protective effect on noise-induced hearing loss in rats. As a conclusion, it is determined that metformin protects from permanent threshold shift in rats. It can be considered a good alternative for protecting noise-induced hearing loss.


Noise Noise-induced hearing loss Acoustic trauma Metformin 



This study was approved by the Medical and Health Sciences Research Council of our university (Project number: DA16/26).


There is no financial support used for this study.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed in the study. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. This study was approved by the institutional ethics committee.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Gülin Gökçen Kesici
    • 1
    Email author
  • Fatma Ceyda Akın Öcal
    • 2
  • Seren Gülşen Gürgen
    • 3
  • Şaban Remzi Erdem
    • 4
  • Ersin Öğüş
    • 5
  • Hatice Seyra Erbek
    • 6
  • Levent Naci Özlüoğlu
    • 6
  1. 1.Department of ENTAnkara Atatürk Education and Research HospitalAnkaraTurkey
  2. 2.Department of ENTGülhane Education and Research HospitalAnkaraTurkey
  3. 3.Department of Histology and EmbryologyCelal Bayar UniversityManisaTurkey
  4. 4.Department of Medical PharmacologyBaşkent UniversityAnkaraTurkey
  5. 5.Department of BiostatisticsBaşkent UniversityAnkaraTurkey
  6. 6.Department of ENTBaşkent UniversityAnkaraTurkey

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