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Intrafollicular melatonin concentration is elevated in patients with ovarian hyperstimulation syndrome (OHSS) and can serve as an important predictor of OHSS

  • Min Zheng
  • Gaoge Zuo
  • Jing Tong
  • Zi-Jiang Chen
  • Wei-Ping Li
  • Cong ZhangEmail author
Gynecologic Endocrinology and Reproductive Medicine
  • 19 Downloads

Abstract

Purpose

Melatonin is an important factor in regulating numerous processes in human female reproduction. The aim of the present study was to compare melatonin levels in the follicular fluid (FF) of ovarian hyperstimulation syndrome (OHSS) women with those of non-OHSS women undergoing in vitro fertilization (IVF)-embryo transfer and to evaluate the relationship between FF melatonin levels and IVF outcomes in these women.

Methods

We determined FF melatonin levels in 20 OHSS women and 23 non-OHSS women on oocyte retrieval day.

Results

OHSS patients had significantly higher melatonin levels as compared to the non-OHSS women (P < 0.001). In addition, melatonin levels of the patients were significantly positively correlated with antral follicle count (AFC), serum anti-Müllerian hormone (AMH) levels, serum estradiol (E2) levels on human chorionic gonadotropin (HCG) administration day, number of retrieved oocytes, total fertilized oocytes, normally fertilized oocytes, cleaved zygotes, top quality embryos on day 3, blastocysts obtained and embryos suitable for transplantation (day 3 embryos + day 5/6 blastocysts) (P < 0.05). While, the intrafollicular melatonin levels were significantly negatively correlated with age, basal serum follicle-stimulating hormone (FSH) levels, serum FSH levels on HCG administration day (P < 0.01). Since younger women with more AFC, higher AMH levels, higher serum E2 levels and larger number of retrieved oocytes are much easier to encounter OHSS, while FF melatonin levels are significantly correlated with these five indices in our study, we propose that intrafollicular melatonin concentration can also be an important predictor of OHSS.

Conclusions

This is the first demonstration that FF melatonin levels were significantly higher in OHSS patients than in non-OHSS group and FF melatonin levels may serve as an important predictor of OHSS.

Keywords

Melatonin OHSS AMH IVF Follicular fluid 

Notes

Author contributions

CZ: project development and editing, MZ: tissue collection, data analysis, manuscript writing and project development, GZ: tissue collection, JT: tissue collection, W-PL: project development, Z-JC: project development.

Funding

This work was supported by a grant from the Major Program of the National Natural Science Foundation of China (81490743) to Z.-J.C.; by grants from National Key R&D Program of China (2017YFC1001403) and the National Natural Science Foundation of China (NSFC: 31671199 and 31871512) to C.Z.; and by the Shanghai Commission of Science and Technology (17DZ2271100).

Compliance with ethical standards

Conflict of interest

All of the authors declare that they have no competing interests.

Ethical approval

All procedures performed in this study involving were in accordance with the ethical standards of the institutional research committee (no. 2015030308) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Center for Reproductive Medicine, School of Medicine, RenJi HospitalShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Key Laboratory of Animal Resistance Research, College of Life ScienceShandong Normal UniversityJinanChina
  3. 3.Shanghai Key Laboratory for Assisted Reproduction and Reproductive GeneticsShanghaiChina
  4. 4.Shanghai Jinshan High SchoolShanghaiChina

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