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Archives of Gynecology and Obstetrics

, Volume 299, Issue 1, pp 229–237 | Cite as

Clinical significance of plasma anti-TOPO48 autoantibody and blood survivin-expressing circulating cancer cells in patients with early stage endometrial carcinoma

  • Xiao-hui Jiang
  • Zou-ying Yao
  • Xu He
  • Jian-bo Zhang
  • Ke Xie
  • Jie Chen
  • Mei Cao
  • Jian Zhang
  • Shang-mian YieEmail author
Gynecologic Oncology

Abstract

Purpose

To examine the clinical significance of an autoantibody (AAb) against a novel tumor-associated antigen (TAA) derived from human DNA-topoisomerase I, termed as TOPO48 AAb, and peripheral blood survivin-expressing circulating cells (CCC) in patients with early stage endometrial cancer (EC).

Methods

Blood samples were collected from 80 patients with early stage EC and 80 age-matched healthy subjects. Plasma levels of the TOPO48 AAb were measured with a specific antibody capture enzyme-linked immunosorbent assay (ELISA) and blood survivin-expressing CCC assessed with a reverse transcription-polymerase chain reaction products based on a hybridization-enzyme-linked immunosorbent assay (RT-PCR–ELISA). Sixty patients were followed up for 36 months after the initial assay test.

Results

There were 75% and 60% samples with positive levels of the TOPO48 AAb and survivin-expressing CCC in the cancer patients, respectively. However, the cumulative positive rate of combination of the two markers was increased to 93.3% with 0.927 (95% CI 0.871–0.984) of area under the curve (AUC) in receiver operating characteristic (ROC) curve analysis. During the follow-up period, patients with positive TOPO48 AAb but negative surviving-expressing CCC had a higher survival rate and a longer survival time than those with negative AAb but positive CCC (P = 0.01).

Conclusions

The combination of TOPO48 AAb and survivin-expressing CCC may be used as a novel recipe to improve the efficiency of early diagnosis and provide more accurate prognostic prediction in patients with early stage EC.

Keywords

Endometrial carcinoma Early diagnosis Prognosis Anti-TOPO48 autoantibody Survivin-expressing circulating cancer cells 

Notes

Author contributions

XHJ, ZYY and XH: data collection, experimental performs, data analysis, manuscript draft writing; KX and JZ: clinical patients and control support; JBZ, JC and MC: experimental support; SMY, project development, data collection, manuscript writing and review

Funding

This study was partially supported by Grant no. KJXM20030826 from the Research and Development Program of the Chengdu Technology Bureau, Chengdu, China, and partly supported by Sichuan Science and Technology Program (Grant no. 2018SZ0144,2016SZ0066, 2018SZ0284 and 2018FZ0043) and West China Second University Hospital of Sichuan University (Grant no. kx027).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study involving were in accordance with the ethical standards of the institutional research committee of the Sichuan Provincial People’s Hospital (No. 20070126,) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Availability of data and materials

We have full control of all primary data and that we agree to allow the Journal to review our data if requested.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Human Sperm BankWest China Second University Hospital of Sichuan UniversityChengduPeople’s Republic of China
  2. 2.Department of Traditional Chinese MedicineLishui Central HospitalLishuiPeople’s Republic of China
  3. 3.Core LaboratorySichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalChengduPeople’s Republic of China
  4. 4.Department of OncologySichuan Academy of Medical Sciences Sichuan Provincial People’s HospitalChengduPeople’s Republic of China
  5. 5.Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of MedicineUniversity of Electronic Science and Technology of ChinaChengduPeople’s Republic of China
  6. 6.Chengdu Cancer Bioengineering Research InstituteChengduPeople’s Republic of China

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