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Archives of Gynecology and Obstetrics

, Volume 298, Issue 4, pp 827–832 | Cite as

Adverse effect of paternal hepatitis B virus infection on clinical pregnancy after frozen–thawed embryo transfer

  • Fan He
  • Lisi Wang
  • Chanyu Zhang
  • Sanglin Li
  • Chengguang Sun
  • Lina Hu
Gynecologic Endocrinology and Reproductive Medicine
  • 56 Downloads

Abstract

Purpose

This study aimed to determine the effect of paternal hepatitis B virus (HBV) infection on reproductive outcomes of couples undergoing frozen–thawed embryo transfer (FET).

Methods

This retrospective cohort study included FET cycles performed between January 2014 and March 2017 in couples with a hepatitis B surface antigen (HBsAg)-positive male partner and an HBsAg-negative female partner, which was categorized as HBsAg group. The FET cycles underwent by couples with both HBsAg-negative partners were randomly selected as controls. The primary outcome was clinical pregnancy.

Results

A total of 117 FET cycles, comprising 39 in the HBsAg group and 78 in the control group, were included. Couples with HBsAg-positive male partners had significantly lower clinical pregnancy rate (17.9 vs 41.0%, P = 0.013), lower implantation rate (11.1 vs 24.5%, P = 0.014), and lower live birth rate (12.8 vs 30.8%, P = 0.034) compared with the control group. Moreover, the multivariate logistic regression analysis showed that paternal HBV infection was negatively associated with clinical pregnancy (odds ratio = 0.297, 95% confidence interval 0.108–0.817, P = 0.019). The miscarriage rate was not significantly different between the two groups (28.6 vs 25.0%, P = 1.000).

Conclusions

Paternal HBV infection resulted in a lower frequency of clinical pregnancy after FET, a difference that was probably attributed to a detrimental effect of HBV on the ability of embryos to survive freezing and thawing.

Keywords

Clinical pregnancy FET HBV Live birth 

Notes

Author contributions

FH: Project development, data analysis and manuscript writing. LSW: Data collection or management and data analysis. CYZ: Project development. SLL: Data collection or management. CGS: Data analysis. LNH: Project development and manuscript editing.

Funding

None.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study was conducted after approval and according to the guidelines of the institutional review board of The Second Affiliated Hospital of Chongqing Medical University (Approval Number: 2018-2). Individual consent to participate in the study was not obtained since it was a medical records-based retrospective study.

References

  1. 1.
    World Health Organization (2017) Hepatitis B. Fact Sheet, Reviewd July 2017. World Health Organization website. http://www.who.int/mediacentre/factsheets/fs204/en/. Accessed 23 Nov 2017
  2. 2.
    Schweitzer A, Horn J, Mikolajczyk RT, Krause G, Ott JJ (2015) Estimations of worldwide prevalence of chronic hepatitis B virus infection: a systematic review of data published between 1965 and 2013. Lancet 386:1546–1555CrossRefPubMedGoogle Scholar
  3. 3.
    Mast EE, Alter MJ, Margolis HS (1999) Strategies to prevent and control hepatitis B and C virus infections: a global perspective. Vaccine 17:1730–1733CrossRefPubMedGoogle Scholar
  4. 4.
    Huang JM, Huang TH, Qiu HY, Fang XW, Zhuang TG, Qiu JW (2002) Studies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes. Asian J Androl 4:209–212PubMedGoogle Scholar
  5. 5.
    Hu XL, Zhou XP, Qian YL, Wu GY, Ye YH, Zhu YM (2011) The presence and expression of the hepatitis B virus in human oocytes and embryos. Hum Reprod 26:1860–1867CrossRefPubMedGoogle Scholar
  6. 6.
    Ye F, Liu Y, Jin Y, Shi J, Yang X, Liu X, Zhang X, Lin S, Kong Y, Zhang L (2014) The effect of hepatitis B virus infected embryos on pregnancy outcome. Eur J Obstet Gynecol Reprod Biol 172:10–14CrossRefPubMedGoogle Scholar
  7. 7.
    Kong Y, Liu Y, Liu X, Li N, Zhu Z, Zhang A, Liu J, Ye F, Lin S (2017) Relationship between the mechanism of hepatitis B virus father–infant transmission and pregnancy outcome. Arch Gynecol Obstet 295:253–257CrossRefPubMedGoogle Scholar
  8. 8.
    Assisted reproductive technology in Europe, 2013: results generated from European registers by ESHRE, Calhaz-Jorge C, De Geyter C, Kupka MS, de Mouzon J, Erb K, Mocanu E, Motrenko T, Scaravelli G, Wyns C, Goossens V (2017) Assisted reproductive technology in Europe, 2013: results generated from European registers by ESHRE. Hum Reprod 32:1957–1973CrossRefGoogle Scholar
  9. 9.
    Sunderam S, Kissin DM, Crawford SB, Folger SG, Jamieson DJ, Warner L, Barfield WD (2017) Assisted reproductive technology surveillance-United States, 2014. MMWR Surveill Summ 66:1–24CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Oger P, Yazbeck C, Gervais A, Dorphin B, Gout C, Jacquesson L, Ayel JP, Kahn V, Rougier N (2011) Adverse effects of hepatitis B virus on sperm motility and fertilization ability during IVF. Reprod Biomed Online 23:207–212CrossRefPubMedGoogle Scholar
  11. 11.
    Shi L, Liu S, Zhao W, Zhou H, Ren W, Shi J (2014) Hepatitis B virus infection reduces fertilization ability during in vitro fertilization and embryo transfer. J Med Virol 86:1099–1104CrossRefPubMedGoogle Scholar
  12. 12.
    Lee VC, Ng EH, Yeung WS, Ho PC (2010) Impact of positive hepatitis B surface antigen on the outcome of IVF treatment. Reprod Biomed Online 21:712–717CrossRefPubMedGoogle Scholar
  13. 13.
    Rienzi L, Gracia C, Maggiulli R, LaBarbera AR, Kaser DJ, Ubaldi FM, Vanderpoel S, Racowsky C (2017) Oocyte, embryo and blastocyst cryopreservation in ART: systematic review and meta-analysis comparing slow-freezing versus vitrification to produce evidence for the development of global guidance. Hum Reprod Update 23:139–155PubMedGoogle Scholar
  14. 14.
    Roque M (2015) Freeze-all policy: is it time for that? J Assist Reprod Genet 32:171–176CrossRefPubMedGoogle Scholar
  15. 15.
    Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology (2011) The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod 26:1270–1283CrossRefGoogle Scholar
  16. 16.
    Bu Z, Kong H, Li J, Wang F, Guo Y, Su Y, Zhai J, Sun Y (2014) Effect of male hepatitis B virus infection on outcomes of in vitro fertilization and embryo transfer treatment: insights from couples undergoing oocyte donation. Int J Clin Exp Med 7:1860–1866PubMedPubMedCentralGoogle Scholar
  17. 17.
    Zhong C, Lu H, Han T, Tan X, Li P, Huang J, Xie Q, Hou Z, Qu T, Jiang Y, Wang S, Xu L, Zhong Y, Huang T (2017) CpG methylation participates in regulation of hepatitis B virus gene expression in host sperm and sperm-derived embryos. Epigenomics 9:123–125CrossRefPubMedGoogle Scholar
  18. 18.
    Zhong Y, Liu DL, Ahmed MMM, Li PH, Zhou XL, Xie QD, Xu XQ, Han TT, Hou ZW, Zhong CY, Huang JH, Zeng F, Huang TH (2017) Host genes regulate transcription of sperm-introduced hepatitis B virus genes in embryo. Reprod Toxicol 73:158–166CrossRefPubMedGoogle Scholar
  19. 19.
    Lucifora J, Arzberger S, Durantel D, Belloni L, Strubin M, Levrero M, Zoulim F, Hantz O, Protzer U (2011) Hepatitis B virus X protein is essential to initiate and maintain virus replication after infection. J Hepatol 55:996–1003CrossRefPubMedGoogle Scholar
  20. 20.
    Harbin Consensus Conference Workshop Group (2014) Improving the Reporting of Clinical Trials of Infertility Treatments (IMPRINT): modifying the CONSORT statement. Fertil Steril 102:952–959CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Center for Reproductive Medicine, Obstetrics and Gynecology DepartmentThe Second Affiliated Hospital of Chongqing Medical UniversityChongqingChina

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