A prospective study on the predictive value of DNA methylation in cervical intraepithelial neoplasia prognosis
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To study the predictive value of the DNA methylation levels of JAM3, SOX1, SLIT2, C13ORF18, and TERT in the cervical intraepithelial neoplasia prognosis.
In the present study, 139 cases were collected and followed up for 24 months. The DNA methylation levels of JAM3, SOX1, SLIT2, C13ORF18, and TERT were tested from their exfoliated cells. One-way ANOVA, receiver operating characteristic (ROC) curve analyses were conducted to analyze the data.
The DNA methylation of the five genes was associated with prognosis of CIN. The levels of methylation increased as the progression of lesion for the prognosis. For CIN1, difference between DNA methylation of JAM3, SOX1, SLIT2, and C13ORF18 had significance statistically (P < 0.001). Sensitivity (95.2%) and specificity (93.1%) of JAM3 were the highest compared with other genes for the prognosis of CIN1. In addition, for CIN2/3, DNA methylation of JAM3, SOX1, SLIT2, TERT, and C13ORF18 had difference statistically (P < 0.001). JAM3 were also the highest in sensitivity (95.2%) and specificity (93.1%) compared with other genes for the prognosis of CIN2/3.
Our data suggest for the first time that DNA methylation levels are associated with prognosis of CIN significantly. DNA methylation levels of some genes, especially JAM3, may serve as markers for the prediction of the CIN prognosis, including CIN1 nature prognosis and CIN2/3 after treatment.
KeywordsCervical intraepithelial neoplasia DNA methylation Prognosis JAM3 Follow-up Prospective study
ZC Guo: data curation, formal analysis, methodology, and writing original draft. YJ Hu: methodology, supervision, writing review, and editing. LQ Yuan: supervision, data curation, formal analysis, writing review, and editing. N Li: supervision, writing review, and editing. T Wang: writing review and editing.
This work was supported by Scientific Research Funding of Tianjin Health Bureau (13KG134).
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest. Ethical approval this article does not contain any studies with human participants performed by any of the authors.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
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