Advertisement

Archives of Dermatological Research

, Volume 311, Issue 4, pp 317–324 | Cite as

Modulation of lipid fluidity likely contributes to the fructose/xylitol-induced acceleration of epidermal permeability barrier recovery

  • Yuki UminoEmail author
  • Sari Ipponjima
  • Mitsuhiro Denda
Original Paper
  • 128 Downloads

Abstract

We previously showed that topical application of hexoses such as fructose accelerates barrier recovery after disruption. We also showed that various hexoses and polyols interact with phospholipid and alter the phase transition temperature. Thus, we hypothesized that the improvement of barrier recovery by hexoses and polyols might be related to the interaction with phospholipid. Here, we tested this idea by examining the effects of xylitol (a component of some skin-care products) and fructose on lipid dynamics in an epidermal-equivalent model at the single-cell level by means of two-photon microscopy after staining with Laurdan, a fluorescent dye sensitive to the physical properties of its membrane environment. First, we confirmed that topical application of xylitol aqueous solution on tape-stripped human skin accelerated barrier recovery. Then, we examined changes of lipid fluidity in the epidermal-equivalent model after application of water or an aqueous solution of xylitol or fructose. Application of xylitol and/or fructose increased the lipid fluidity in the uppermost part of the stratum granulosum layer, compared to treatment with water alone, and accelerated the exocytosis of lamellar bodies to the intercellular domain between stratum corneum and stratum granulosum. Our results support the idea that the improvement of epidermal barrier homeostasis upon topical application of xylitol or fructose is due to increased lipid fluidity in the uppermost layer of the stratum granulosum, which enables accelerated release of lipid from the stratum granulosum, thereby improving the lamellar structure and accelerating epidermal permeability barrier recovery.

Keywords

Lipid fluidity Epidermal permeability barrier Stratum granulosum Lamellar body Two-photon microscope 

Notes

Acknowledgements

We appreciate the feedback offered by Dr. Makiko Goto (Shiseido Global Innovation Center). This work was supported by JST CREST Grant Number JPMJCR15D2, Japan.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

The protocol was approved by the ethics committees of Shiseido Research Center, and was in accordance with the National Institute of Health guideline and Declaration of Helsinki principles.

Informed consent

Informed consent was obtained from all individual participants.

Supplementary material

403_2019_1905_MOESM1_ESM.tif (8.1 mb)
Supplementary material 1 (TIF 8267 KB)
403_2019_1905_MOESM2_ESM.docx (19 kb)
Supplementary material 2 (DOCX 18 KB)

References

  1. 1.
    Denda M (2011) Effects of topical application of aqueous solutions of hexoses on epidermal permeability barrier recovery rate after barrier disruption. Exp Dermatol 20:943–944CrossRefGoogle Scholar
  2. 2.
    Denda M, Fuziwara S, Inoue K (2003) Influx of calcium and chloride ions into epidermal keratinocytes regulates exocytosis of epidermal lamellar bodies and skin permeability barrier homeostasis. J Invest Dermatol 121:362–367CrossRefGoogle Scholar
  3. 3.
    Denda M, Fuziwara S, Inoue K (2004) Association of cyclic adenosine monophosphate with permeability barrier homeostasis of murine skin. J Invest Dermatol 122:140–146CrossRefGoogle Scholar
  4. 4.
    Denda M, Kitamura K, Elias PM, Feingold KR (1997) Trans-4-(Aminomethyl)cyclohexane carboxylic acid (T-AMCHA), an anti-fibrinolytic agent, accelerates barrier recovery and prevents the epidermal hyperplasia induced by epidermal injury in hairless mice and humans. J Invest Dermatol 109:84–90CrossRefGoogle Scholar
  5. 5.
    Denda M, Koyama J, Namba R, Horii I (1994) Stratum corneum lipid morphology and transepidermal water loss in normal skin and surfactant-induced scaly skin. Arch Dermatol Res 286:41–46CrossRefGoogle Scholar
  6. 6.
    Denda M, Sato J, Masuda Y, Tsuchiya T, Koyama J, Kuramoto M, Elias PM, Feingold KR (1998) Exposure to a dry environment enhances epidermal permeability barrier function. J Invest Dermatol 111:858–863CrossRefGoogle Scholar
  7. 7.
    den Hollander L, Han H, de Winter M, Svensson L, Masich S, Daneholt B, Norlén L (2016) Skin lamellar bodies are not discrete vesicles but part of a tubuloreticular network. Acta Derm Venereol 96:303–308CrossRefGoogle Scholar
  8. 8.
    Elias PM (2006) The epidermal permeability barrier from Saran Wrap to biosensor. In: Skin B, Elias PM, Feingold KR (eds), 2. Taylor & Francis, New York, pp 25–31Google Scholar
  9. 9.
    Elias PM, Cullander C, Mauro T, Rassner U, Kömüves L, Brown BE, Menon GK (1998) The secretory granular cell: the outermost granular cell as a specialized secretory cell. J Investig Dermatol Symp Proc 3:87–100CrossRefGoogle Scholar
  10. 10.
    Feingold KR, Elias PM (2014) Role of lipids in the formation and maintenance of the cutaneous permeability barrier. Biochim Biophys Acta 1841:280–294CrossRefGoogle Scholar
  11. 11.
    Kalia YN, Pirot F, Guy RH (1996) Homogeneous transport in a heterogeneous membrane: water diffusion across human stratum corneum in vivo. Biophys J 71:2692–2700CrossRefGoogle Scholar
  12. 12.
    Korponyai C, Szél E, Behány Z, Varga E, Mohos G, Dura Á, Dikstein S, Kemény L, Erős G (2017) Effects of locally applied glycerol and xylitol on the hydration, barrier function and morphological parameters of the skin. Acta Derm Venereol 97:182–187CrossRefGoogle Scholar
  13. 13.
    Kumamoto J, Nakanishi S, Umino Y, Denda M (2018) Removal of nontoxic foreign material to the surface by cultured human epidermal keratinocytes in an epidermal-equivalent model. J Dermatol Sci 89:97–99CrossRefGoogle Scholar
  14. 14.
    Mazeres S, Fereidouni F, Joly E (2017) Using spectral decomposition of the signals from laurdan-derived probes to evaluate the physical state of membranes in live cells. F1000 Res 6:763CrossRefGoogle Scholar
  15. 15.
    Nakata S, Deguchi A, Seki Y, Furuta M, Fukuhara K, Nishihara S, Inoue K, Kumazawa N, Mashiko S, Fujihira S, Goto M, Denda M (2015) Characteristic responses of a phospholipid molecular layer to polyols. Colloid Surf B Biointerfaces 136:594–599CrossRefGoogle Scholar
  16. 16.
    Nakata S, Nomura M, Yamaguchi Y, Hishida M, Kitahata H, Katsumoto Y, Denda M, Kumazawa N (2019) Characteristic responses of a 1,2-dipalmitoleoyl-sn-glycero-3- phosphoethanolamine molecular layer depending on the number of CH(OH) groups in polyols. Colloids Surf A Physicochem Eng Asp 560:149–153CrossRefGoogle Scholar
  17. 17.
    Nakata S, Shiota T, Kumazawa N, Denda M (2012) Interaction between a monosaccharide and a phospholipid molecular layer. Colloids Surf A 405:14–18CrossRefGoogle Scholar
  18. 18.
    Norlén L (2001) Skin barrier formation: the membrane folding model. J Invest Dermatol 117:823–829CrossRefGoogle Scholar
  19. 19.
    Sanchez SA, Tricerri MA, Gratton E (2012) Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo. Proc Natl Acad Sci USA 109:7314–7319CrossRefGoogle Scholar
  20. 20.
    Sato J, Denda M, Nakanishi J, Koyama J (1998) Dry condition affects desquamation of stratum corneum in vivo. J Dermatol Sci 18:163–169CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Shiseido Global Innovation CenterYokohamaJapan
  2. 2.Research Institute for Electronic ScienceHokkaido UniversitySapporoJapan
  3. 3.Shiseido Global Innovation CenterYokohamaJapan

Personalised recommendations