Malondialdehyde and advanced oxidation protein products are not increased in psoriasis: a controlled study
- 52 Downloads
This study investigated oxidative stress in patients with psoriasis of low and medium disease activity. We measured advanced oxidation protein products (AOPP) and malondialdehyde (MDA) in plasma using UV-spectrophotometry and high performance liquid chromatography connected to a fluorescence detector in 84 patients and 84 matched healthy subjects. AOPP is a marker of protein oxidation due to inflammation, whereas MDA is a hydroxyl radical initiated lipid peroxidation product. Clinico-demographic variables including age, gender, disease severity, and fatigue were assessed in relation to AOPP and MDA. Disease severity was evaluated with the Psoriasis Area and Severity Index and the Dermatology Life Quality Index. Median (interquartile range, IQR) AOPP concentrations were 66 µmol/l (IQR 54–102) in patients and 69 µmol/l (IQR 55–87) in healthy subjects (P = 0.75). Median plasma MDA concentrations were significantly lower in patients than in healthy subjects (0.68 µM, IQR 0.54–0.85 vs. 0.76 µM, IQR 0.60–0.97; P = 0.03). Plasma levels of AOPP and MDA did not indicate oxidative stress in patients with mild psoriasis. Higher AOPP concentrations were associated with male gender, high body mass index, and high hemoglobin values. Elevated MDA concentrations were associated with advanced age and male gender. No associations with disease severity were detected. Although, the two selected biomarkers do not provide a complete measure of oxidative damage, our study demonstrates that a number of physiological and methodological factors influence the levels of MDA and AOPP. Such methodological issues are important to consider when interpreting results using these biomarkers in patients with psoriasis.
KeywordsOxidative stress Advanced oxidation protein products AOPP Malondialdehyde MDA Psoriasis
Advanced oxidation protein products
Reactive oxygen species
Psoriasis area and severity index
Quality of life
Dermatology life quality index
Fatigue visual analog scale
The depression subscale of the Hospital Anxiety and Depression Scale
Thiobarbituric acid reactive substances
High-performance liquid chromatography connected to a fluorescence detector
Inger Marie Skoie received support as a doctoral research fellow from the Western Norway Regional Health Authority (WNRA, “Helse Vest”), Grant number (912015).
Compliance with ethical standards
Conflict of interest
The authors report no conflicts of interest.
This study was approved by the Regional Committee for Medical Research Ethics in Norway (REK vest 2010/1455). All participants provided written informed consent, and the study was conducted in accordance with the latest revision of the Helsinki Declaration.
- 19.Halliwell B, Gutteridge JMC (2007) Free radicals in biology and medicine, 4th edn. Oxford University Press, OxfordGoogle Scholar
- 22.Kilinc Karaarslan I, Girgin Sagin F, Ertam I, Alper S, Ozturk G, Yildirim Sozmen E (2006) Broad-band ultraviolet B phototherapy is associated with elevated serum thiobarbituric acid reactive substance and nitrite-nitrate levels in psoriatic patients. J Eur Acad Dermatol Venereol 20:1226–1231. https://doi.org/10.1111/j.1468-3083.2006.01765.x CrossRefGoogle Scholar
- 23.Krzystek-Korpacka M, Patryn E, Boehm D, Berdowska I, Zielinski B, Noczynska A (2008) Advanced oxidation protein products (AOPPs) in juvenile overweight and obesity prior to and following weight reduction. Clin Biochem 41:943–949. https://doi.org/10.1016/j.clinbiochem.2008.04.024 CrossRefGoogle Scholar
- 27.Lykkesfeldt J (2001) Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible spectrophotometry. Clin Chem 47:1725–1727Google Scholar
- 30.Nagy LE (2015) The role of innate immunity in alcoholic liver disease. Alcohol Res 37:237–250Google Scholar
- 42.Valli A, Suliman ME, Meert N, Vanholder R, Lindholm B, Stenvinkel P et al (2007) Overestimation of advanced oxidation protein products in uremic plasma due to presence of triglycerides and other endogenous factors. Clin Chim Acta 379:87–94. https://doi.org/10.1016/j.cca.2006.12.026 CrossRefGoogle Scholar
- 46.Wolfe F (2004) Fatigue assessments in rheumatoid arthritis: comparative performance of visual analog scales and longer fatigue questionnaires in 7760 patients. J Rheumatol 31:1896–1902Google Scholar
- 47.Wozniak A, Drewa G, Krzyzynska-Malinowska E, Czajkowski R, Protas-Drozd F, Mila-Kierzenkowska C et al (2007) Oxidant-antioxidant balance in patients with psoriasis. Med Sci Monit 13:CR30–CR33Google Scholar