CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration
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Melanocyte stem cells (McSCs) are localized in the bulge region of hair follicles and supply melanocytes, which determine hair color by synthesizing melanin. Ectopic differentiation of McSCs, which are usually undifferentiated in the bulge region, causes depletion of McSCs and results in hair graying. Therefore, to prevent hair graying, it is essential to maintain McSCs in the bulge region, but the mechanism of McSC maintenance remains unclear. To address this issue, we investigated the role of CXCL12, a chemokine which was previously suggested to induce migration of melanocyte lineage cells, as a niche component of McSCs. Immunohistological analysis revealed that CXCL12 was highly expressed in the bulge region of human hair follicles. CXCL12 mRNA expression level was significantly lower in white hairs plucked from human scalps than in black hairs. CXCL12 attracted the migration of early-passage normal human epidermal melanocytes (eNHEMs), an in vitro model of McSCs, which had characteristics of immature melanocyte precursors. We also found that CXCL12 suppressed their differentiation. These results suggest that CXCL12 regulates differentiation of McSCs as well as their proper localization, and maintaining McSCs by regulating CXCL12 expression level in the bulge region may be a key to preventing hair graying.
KeywordsMelanocyte stem cell CXCL12 Stem cell niche Differentiation Bulge
We are grateful to N. Goshima (Cellisis Co., Ltd., Aichi, Japan) for her support in preparing the manuscript.
TY and SH designed the research study. TY, MA, YI, TK, SN and NY performed the research. TY, SH, SN, KS and HA carried out the data analysis and wrote the manuscript. All authors critically revised the manuscript and approved the final manuscript.
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Conflict of interest
The authors state no conflict of interest.
- 5.Choi YJ, Yoon TJ, Lee YH (2008) Changing expression of the genes related to human hair graying. Eur J Dermatol 18:397–399Google Scholar
- 7.Ghosh MC, Collins GD, Vandanmagsar B, Patel K, Brill M, Carter A, Lustig A, Becker KG, Wood WW III, Emeche CD, French AD, O’Connell MP, Xu M, Weeraratna AT, Taub DD (2009) Activation of Wnt5A signaling is required for CXC chemokine ligand 12-mediated T-cell migration. Blood 114:1366–1373CrossRefGoogle Scholar
- 22.Yamauchi A, Hadjur C, Takahashi T, Suzuki I, Hirose K, Mahe YF (2013) Human skin melanocyte migration towards stromal cell-derived factor-1α demonstrated by optical real-time cell mobility assay: modulation of their chemotactic ability by α-melanocyte-stimulating hormone. Exp Dermatol 22:664–667CrossRefGoogle Scholar