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Archives of Dermatological Research

, Volume 310, Issue 9, pp 729–735 | Cite as

Age-related and seasonal changes in covalently bound ceramide content in forearm stratum corneum of Japanese subjects: determination of molecular species of ceramides

  • Anna Fujiwara
  • Masashi Morifuji
  • Masami Kitade
  • Keiko Kawahata
  • Tomoyuki Fukasawa
  • Taketo Yamaji
  • Hiroyuki Itoh
  • Makoto Kawashima
Original Paper
  • 99 Downloads

Abstract

The stratum corneum (SC) consists of corneocytes surrounded by a neutral lipid-enriched intercellular matrix. Ceramides represent approximately 50% of intercellular lipids, and play important roles in retaining epidermal water. The SC also contains covalently bound ceramides, which are thought to play a crucial role in the formation of lamellar structures, and are involved in maintaining skin barrier function. A previous report showed that levels of free ceramides in human SC changed with the seasons and age, although whether the content of different species of covalently bound ceramides also underwent such temporal changes was unclear. Here, SC samples were taken from 99 healthy individuals of different ages (24–64 years) and during different seasons. The content of different molecular species of covalently bound ceramides in the samples was quantified using HPLC–MS/MS. The levels of total covalently bound ceramides (Total-Cers) significantly decreased approximately 50% in autumn and winter, compared with that of spring and summer. The levels of covalently bound ceramides containing saturated fatty acids (SFA-Cers) in the spring and summer were approximately 2.3-fold higher than that seen in autumn and winter, whereas the level of covalently bound ceramides containing unsaturated fatty acids (USFA-Cers) in spring and summer were approximately 1.6-fold higher than that in autumn and winter. Furthermore, the ratio between SFA-Cers and USFA-Cers was significantly lower in spring and summer than in autumn and winter. The levels of SFA-Cers, but not USFA-Cers, were significantly lower in individuals ≥ 50 years old compared to those who are 30- and 40-years old in the spring. Our study showed for the first time that, similar to free ceramides, the level of covalently bound ceramides changed with the seasons. However, age-related changes in covalently bound ceramide content were limited in that only the amount of SFA-Cers in the spring was lower in older individuals.

Keywords

Covalently bound ceramides Molecular species Stratum corneum Seasons Age Human 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in these studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Anna Fujiwara
    • 1
  • Masashi Morifuji
    • 1
  • Masami Kitade
    • 1
  • Keiko Kawahata
    • 1
  • Tomoyuki Fukasawa
    • 1
  • Taketo Yamaji
    • 1
  • Hiroyuki Itoh
    • 1
  • Makoto Kawashima
    • 2
  1. 1.R&D DivisionMeiji Co., Ltd.HachioujiJapan
  2. 2.Department of DermatologyTokyo Women’s Medical University, School of MedicineTokyoJapan

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