Different Trypanosoma cruzi calreticulin domains mediate migration and proliferation of fibroblasts in vitro and skin wound healing in vivo
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Calreticulin is an endoplasmic reticulum-resident, calcium-binding, stress-produced, chaperone protein that serves multiple functions and is widely distributed in eukaryotic cells. Exogenously applied recombinant calreticulin solution, markedly enhanced the rate and quality of skin wound healing. These modulatory effects are more efficient than commercially available topic platelet-derived growth factor ointments (Regranex®). Trypanosoma cruzi calreticulin is more effective in equimolar terms to human counterpart in accelerating skin wound healing. While the effect of externally added recombinant parasite calreticulin on wound healing has been reported, the domains responsible for these modulatory effects have not yet been established. Here, recombinant parasite calreticulin and some of its domains were tested to assess their influence in increasing proliferation and migration of fibroblasts in vitro and rat skin wound healing in vivo. Herein, we propose that Trypanosoma cruzi whole calreticulin or some of its domains are differentially involved in the modulation of wound-healing cell migration and proliferation, and cosmetic outcome. Therefore, precise combination of the parasite protein and its domains could allow us to tailor-specific desired effects during the skin wound-healing process.
KeywordsTrypanosoma cruzi Calreticulin Domains Proliferation Migration Skin wound healing
Research in the author’s laboratory was supported by the FONDECYT 11130257 Grant of CONICYT Chile. We thank Dr. David Carrino for his helpful suggestions in the preparation of this manuscript.
Compliance with ethical standards
Research involving human and animal rights
All animal procedures were approved by the local Animal Bioethics Committee in accordance with the International Guiding Principles for Biomedical Research Involving Animals, were developed by the Council for International Organizations of Medical Sciences (CIOMS), and were supervised by trained veterinary medical staff.
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