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The association between GJB2 gene polymorphism and psoriasis: a verification study

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Abstract

Psoriasis is a chronic inflammatory skin disease with multifactorial etiology. Connexin 26 (Cx26), an important gap junction protein, has been found highly expressed in plaques of psoriasis. Recently, genome wide association studies (GWAS) identified one new single nucleotide polymorphism (SNP) in GJB2 gene coding for Cx26 protein associated with psoriasis in Chinese Han population. In this paper, we verified the GWAS data in Chinese Han population. Here we genotyped the polymorphism of GJB2 rs3751385:C>T in 371 psoriasis patients and 330 healthy controls in Chinese Han population using polymerase chain reaction restriction fragment length polymorphism assay (PCR–RFLP). Our case–control assay indicated decreased frequency of the GJB2 rs3751385 C allele in psoriasis patients compared with that in the healthy controls [p = 6.02 × 10−5, Odds ratio (OR) = 0.793, 95 % confidence interval (CI) 0.706–0.889]. The result suggested that GJB2 gene polymorphism rs3751385:C>T was associated with psoriasis susceptibility of Chinese Han population.

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Abbreviations

Cx26:

Connexin 26

GWAS:

Genome-wide association studies

SNP:

Single nucleotide polymorphism

GJB2 :

Gap junction beta 2

PCR-RFLP:

Polymerase chain reaction-restriction fragment length polymorphism assay

OR:

Odds ratio

CI:

Confidence interval

PASI:

Psoriasis area and severity index

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Acknowledgments

We thank Hong fu Xie, Ming liang Chen, Wei Shi, Wu Zhu, Bo Deng, Huan Chen, Jiang lin Zhang, Dan Jian, JieLi (The Department of Dermatology) for their helps with collection of clinic samples. We thank Mu Zi Li (Department of Hypertension Research in Xiang-Ya Hospital, Central South University) for technical supports. We thank Xiao Ping Chen (Department of Pharmacology, School of Pharmaceutical Science, and Central South University) for instructions on the experiments. Contract grant sponsor: the National Science Fund for Distinguished Young Scholars; National Natural Science Foundation of China (Grant number: 81071290); the Fundamental Research Funds for the Central Universities (Grant number: 2011JQ012; 2012QNZT108).

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Authors and Affiliations

  1. Department of Dermatology, Xiang-Ya Hospital Central South University, 87 XiangYa Road, Changsha, 410008, Hunan, People’s Republic of China

    Que-Ping liu, Li-Sha Wu, Fang-Fang Li, Shuang Liu, Juan Su, Ye-Hong Kuang, Chen Chen, Xiao-Yun Xie, Ming-Hao Jiang, Shuang zhao, Ming-Liang Chen & Xiang Chen

  2. Department of Dermatology, Hengyang Center Hospital Southern Medical University, 12 YanCheng Road, Hengyang, 421001, Hunan, People’s Republic of China

    Que-Ping liu

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  1. Que-Ping liu
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  2. Li-Sha Wu
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  3. Fang-Fang Li
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  4. Shuang Liu
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  5. Juan Su
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  6. Ye-Hong Kuang
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  7. Chen Chen
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  8. Xiao-Yun Xie
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  9. Ming-Hao Jiang
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  10. Shuang zhao
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  11. Ming-Liang Chen
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  12. Xiang Chen
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Corresponding author

Correspondence to Xiang Chen.

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Que-Ping liu, Li-Sha Wu, and Fang-Fang Li contributed equally to this work.

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liu, QP., Wu, LS., Li, FF. et al. The association between GJB2 gene polymorphism and psoriasis: a verification study. Arch Dermatol Res 304, 769–772 (2012). https://doi.org/10.1007/s00403-012-1273-x

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  • DOI: https://doi.org/10.1007/s00403-012-1273-x

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