Incorporation of gold nanoparticles into the bilayer of polydiacetylene unilamellar vesicles
- 51 Downloads
Gold nanoparticles made with three different alkanethiol surface passivating agents were loaded into the hydrophobic bilayers of polydiacetylene liposomes. The physical and optical properties of the vesicles were compared to each other and a nanoparticle-free control. Small gold nanoparticles were utilized to minimize the plasmon absorption and thereby minimize the effects of energy transfer processes on the optical behavior of the polydiacetylene moiety. The size and structure of the liposomes were examined with dynamic light scattering and electron microscopy, while the stability of the bilayer was investigated through differential scanning calorimetry. Optical spectroscopy was used to monitor the photopolymerization of the vesicles as well as the stress-induced polydiacetylene blue to red transition. Specifically, changes to the absorption frequency, fluorescence frequency, and the fluorescence intensity were monitored. This system can serve as a model system for the optimization of liposomes containing nanoparticles with specialized optical, magnetic, or chemical functionality.
KeywordsPolydiacetylene Liposome Gold nanoparticle Fluorescence Electron microscopy
The authors gratefully acknowledge the assistance of Steven Myles, Michelin Americas Research Center, for assistance with TEM data collection.
This work was supported in part by the National Science Foundation EPSCoR Program under NSF Award # OIA-1655740.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect those of the National Science Foundation.
- 2.Lasic DD (1995) Applications of Liposomes. In: R. Lipowsky and E. Sackmann, (eds) Handbook of Biological Physics, Elsevier ScienceGoogle Scholar
- 9.Perche F, Torchilin VP (2013) Recent trends in multifunctional liposomal nanocarriers for enhanced yumor targeting. J Drug Deliv Article ID 705265. https://doi.org/10.1155/2013/705265
- 13.Baughman RH, Yee KC (1978) Solid-state polymerization of linear and cyclic acetylenes. J Poly Sci D:Macromol Rev 13:219–239Google Scholar
- 14.Bloor D, Chance RR (eds) (1985) Polydiacetylenes: synthesis, structure and electronic properties. Martinus Nijhoff, BostonGoogle Scholar
- 16.Giorgetti E, Muniz-Miranda M, Margheri G, Giusti A, Sottini S, Alloisio M, Cuniberti C, Dellepiane G (2006) UV polymerization of self-assembled monolayers of a novel diacetylene on silver: a spectroscopic analysis by surface plasmon resonance and surface enhanced Raman scattering. Langmuir 22:1129–1134CrossRefGoogle Scholar
- 42.Wright-Walker CJ, Hansen CE, Evans MA, Nyers ES, Hanks TW (2013) Efficient production of fluorescent polydiacetylene-containing liposomes for pathogen detection and identification. MRS Proc 1569:mrss13–mr1569-qq01–03. https://doi.org/10.1557/opl2013.1099