Colloid and Polymer Science

, Volume 296, Issue 9, pp 1555–1571 | Cite as

Hybrid collagen/pNIPAAM hydrogel nanocomposites for tissue engineering application

  • Nistor Manuela Tatiana
  • Vasile CorneliaEmail author
  • Rodica Tatia
  • Chiriac Aurica
Original Contribution


The present work explores new hybrid bioactive, enzymatically degradable hydrogel nanocomposites. They are composed by stimuli-sensitive semi-interpenetrating collagen/poly(N-isopropyl acrylamide) polymeric matrix, Dellite® 67G and Cloisite® 93A nanoclays, and hydroxyapatite particles. Morphology of the hybrid hydrogel nanocomposites was examined by near infrared chemical imaging, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy techniques. It has been found that the porous hydrogel nanocomposites showed a mix between intercalated and exfoliated structure, with interconnected pores and the inorganic material was evenly distributed in the polymeric matrix because of specific interactions between components. The collagen resistance against enzymatic degradation and the thermal stability were improved due to the protein encapsulation in the synthetic polymer matrix. The hydrogel nanocomposites showed suitable swelling characteristics for tissue engineering. In vitro cytocompatibility and cell viability revealed that the hybrid nanocomposites were non-cytotoxic for rat osteoblasts. These hybrid nanocomposites were designed as potential synthetic bone tissue engineering.

Graphical Abstract


Hydrogels Nanocomposites Tissue engineering Reinforcement Enzymatic degradation Swelling 



The research leading to these results has received funding from the Romanian National Authority for Scientific Research, CNCS-UEFISCDI, project BIONANOMED 164/2012.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

396_2018_4367_MOESM1_ESM.pptx (63 kb)
ESM 1 (PPTX 62 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Nistor Manuela Tatiana
    • 1
  • Vasile Cornelia
    • 1
    Email author
  • Rodica Tatia
    • 2
  • Chiriac Aurica
    • 1
  1. 1.Department of Physical Chemistry of Polymers“Petru Poni” Institute of Macromolecular ChemistryIasiRomania
  2. 2.National Institute for Biological SciencesBucharestRomania

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