Associations among dietary seaweed intake, c-MYC rs6983267 polymorphism, and risk of colorectal cancer in a Korean population: a case–control study
- 12 Downloads
The effects of seaweed compounds have been studied in relation to colorectal cancer (CRC) based on their ability to modulate carcinogen metabolism in vivo and in vitro. However, no epidemiological studies on the interaction between edible seaweed and genetic variants relevant to CRC have been reported. This study examined the associations among dietary seaweed intake (gim, miyeok, and dashima), single-nucleotide polymorphisms (SNPs; rs6983267, rs7014346, and rs719725), and CRC risk in a Korean population.
The participants comprised 923 CRC patients and 1846 controls who visited the National Cancer Center Korea. We used a Semiquantitative Food Frequency Questionnaire and genotyped SNPs using genomic DNA samples.
The intake of total seaweed, miyeok, and dashima showed a significant inverse association with CRC risk after adjusting for potential confounding factors (total seaweed odds ratio (OR) [95% CI] = 0.65 [0.50–0.85], P for trend < 0.001; miyeok = 0.82 [0.62–1.09], P for trend < 0.05; dashima = 0.58 [0.44–0.76], P for trend < 0.001, highest vs. lowest tertile). We confirmed that the homozygous T/T allele of rs6983267 c-MYC indicated an interaction between dietary seaweed intake and both overall CRC and rectal cancer (CRC OR [95% CI] = 0.52 [0.34–0.81], P for interaction = 0.015; rectal cancer = 0.45 [0.25–0.79], P for interaction = 0.007, T/T carriers with high total seaweed intake vs. T/T carriers with low total seaweed intake).
This study provides evidence of the effect of dietary seaweed intake on CRC risk with respect to c-MYC gene variants.
KeywordsSeaweed c-MYC rs6983267 Genetic polymorphism Colorectal cancer
Body mass index
Genome-wide association study
Semiquantitative Food Frequency Questionnaire
We are thankful to all participants involved in this study. This research was supported by grants from the National Cancer Center in Korea (1710882, 1810090).
The authors’ responsibilities were as follows: JMK and JSK designed the research, performed the statistical analysis, and had primary responsibility for the final content; JL, JHO, HJC, DKS, and AS recruited the study participants, collected the data, and conducted the research; JMK and JSK wrote the manuscript; and all authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors (Jimi Kim, Jeonghee Lee, Jae Hwan Oh, Hee Jin Chang, Dae Kyung Sohn, Aesun Shin, and Jeongseon Kim) declare that they have no conflicts of interest.
Written and signed informed consent was obtained from all patients in adherence to the National Cancer Center Korea guidelines. This study was approved by the Institutional Review Board of the National Cancer Center Korea (IRB no. NCCNCS-10-350 and no. NCC2015-0202).
- 3.Hughes LAE, Simons C, van den Brandt PA, van Engeland M, Weijenberg MP (2017) Lifestyle, diet, and colorectal cancer risk according to (Epi)genetic instability: current evidence and future directions of molecular pathological epidemiology. Curr Colorectal Cancer Rep 13(6):455–469. https://doi.org/10.1007/s11888-017-0395-0 CrossRefGoogle Scholar
- 11.Tomlinson I, Webb E, Carvajal-Carmona L, Broderick P, Kemp Z, Spain S, Penegar S, Chandler I, Gorman M, Wood W et al (2007) A genome-wide association scan of tag SNPs identifies a susceptibility variant for colorectal cancer at 8q24.21. Nat Genet 39(8):984–988. https://doi.org/10.1038/ng2085 CrossRefGoogle Scholar
- 12.Tenesa A, Farrington SM, Prendergast JG, Porteous ME, Walker M, Haq N, Barnetson RA, Theodoratou E, Cetnarskyj R, Cartwright N et al (2008) Genome-wide association scan identifies a colorectal cancer susceptibility locus on 11q23 and replicates risk loci at 8q24 and 18q21. Nat Genet 40(5):631–637. https://doi.org/10.1038/ng.133 CrossRefGoogle Scholar
- 13.Zanke BW, Greenwood CM, Rangrej J, Kustra R, Tenesa A, Farrington SM, Prendergast J, Olschwang S, Chiang T, Crowdy E et al (2007) Genome-wide association scan identifies a colorectal cancer susceptibility locus on chromosome 8q24. Nat Genet 39(8):989–994. https://doi.org/10.1038/ng2089 CrossRefGoogle Scholar
- 14.Poynter JN, Figueiredo JC, Conti DV, Kennedy K, Gallinger S, Siegmund KD, Casey G, Thibodeau SN, Jenkins MA, Hopper JL et al (2007) Variants on 9p24 and 8q24 are associated with risk of colorectal cancer: results from the Colon Cancer Family Registry. Cancer Res 67(23):11128–11132. https://doi.org/10.1158/0008-5472.can-07-3239 CrossRefGoogle Scholar
- 15.Nan H, Morikawa T, Suuriniemi M, Imamura Y, Werner L, Kuchiba A, Yamauchi M, Hunter DJ, Kraft P, Giovannucci EL et al (2013) Aspirin use, 8q24 single nucleotide polymorphism rs6983267, and colorectal cancer according to CTNNB1 alterations. J Natl Cancer Inst 105(24):1852–1861. https://doi.org/10.1093/jnci/djt331 CrossRefGoogle Scholar
- 16.Xiong F, Wu C, Bi X, Yu D, Huang L, Xu J, Zhang T, Zhai K, Chang J, Tan W et al (2010) Risk of genome-wide association study-identified genetic variants for colorectal cancer in a Chinese population. Cancer Epidemiol Biomark Prev 19(7):1855–1861. https://doi.org/10.1158/1055-9965.EPI-10-0210 CrossRefGoogle Scholar
- 19.Zorofchian Moghadamtousi S, Karimian H, Khanabdali R, Razavi M, Firoozinia M, Zandi K, Abdul Kadir H (2014) Anticancer and antitumor potential of fucoidan and fucoxanthin, two main metabolites isolated from brown algae. Sci World J 2014:768323. https://doi.org/10.1155/2014/768323 CrossRefGoogle Scholar
- 28.Michikawa T, Inoue M, Shimazu T, Sawada N, Iwasaki M, Sasazuki S, Yamaji T, Tsugane S, Japan Public Health Center-based Prospective Study G (2012) Seaweed consumption and the risk of thyroid cancer in women: the Japan Public Health Center-based Prospective Study. Eur J Cancer Prev 21(3):254–260. https://doi.org/10.1097/CEJ.0b013e32834a8042 CrossRefGoogle Scholar
- 33.Hosokawa M, Kudo M, Maeda H, Kohno H, Tanaka T, Miyashita K (2004) Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPAR gamma ligand, troglitazone, on colon cancer cells. Biochim Biophys Acta 1675(1–3):113–119. https://doi.org/10.1016/j.bbagen.2004.08.012 CrossRefGoogle Scholar
- 35.Das SK, Hashimoto T, Shimizu K, Yoshida T, Sakai T, Sowa Y, Komoto A, Kanazawa K (2005) Fucoxanthin induces cell cycle arrest at G0/G1 phase in human colon carcinoma cells through up-regulation of p21WAF1/Cip1. Biochim Biophys Acta 1726(3):328–335. https://doi.org/10.1016/j.bbagen.2005.09.007 CrossRefGoogle Scholar
- 40.Hutter CM, Slattery ML, Duggan DJ, Muehling J, Curtin K, Hsu L, Beresford SA, Rajkovic A, Sarto GE, Marshall JR et al (2010) Characterization of the association between 8q24 and colon cancer: gene-environment exploration and meta-analysis. BMC Cancer 10:670. https://doi.org/10.1186/1471-2407-10-670 CrossRefGoogle Scholar
- 41.Li L, Lv L, Liang Y, Shen X, Zhou S, Zhu J, Ma R (2015) Association of 8q23-24 region (8q23.3 loci and 8q24.21 loci) with susceptibility to colorectal cancer: a systematic and updated meta-analysis. Int J Clin Exp Med 8(11):21001–21013Google Scholar
- 42.Tuupanen S, Niittymaki I, Nousiainen K, Vanharanta S, Mecklin JP, Nuorva K, Jarvinen H, Hautaniemi S, Karhu A, Aaltonen LA (2008) Allelic imbalance at rs6983267 suggests selection of the risk allele in somatic colorectal tumor evolution. Cancer Res 68(1):14–17. https://doi.org/10.1158/0008-5472.can-07-5766 CrossRefGoogle Scholar
- 43.Tuupanen S, Turunen M, Lehtonen R, Hallikas O, Vanharanta S, Kivioja T, Bjorklund M, Wei G, Yan J, Niittymaki I et al (2009) The common colorectal cancer predisposition SNP rs6983267 at chromosome 8q24 confers potential to enhanced Wnt signaling. Nat Genet 41(8):885–890. https://doi.org/10.1038/ng.406 CrossRefGoogle Scholar
- 48.Sugimachi K, Niida A, Yamamoto K, Shimamura T, Imoto S, Iinuma H, Shinden Y, Eguchi H, Sudo T, Watanabe M et al (2014) Allelic imbalance at an 8q24 oncogenic SNP is involved in activating MYC in human colorectal cancer. Ann Surg Oncol 21(Suppl 4):S515–521. https://doi.org/10.1245/s10434-013-3468-6 CrossRefGoogle Scholar