Chronic consumption of the dietary polyphenol chrysin attenuates metabolic disease in fructose-fed rats
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Metabolic syndrome (MS) is a major public health issue worldwide and fructose consumption has been associated with MS development. Recently, we showed that the dietary polyphenol chrysin is an effective inhibitor of fructose uptake by human intestinal epithelial cells. Therefore, our aim was to investigate if chrysin interferes with the development of MS induced by fructose in an animal model.
Adult male Sprague–Dawley rats (220–310 g) were randomly divided into four groups: (A) tap water (control), (B) tap water and a daily dose of chrysin (100 mg/kg) by oral administration (chrysin) (C) 10% fructose in tap water (fructose), and (D) 10% fructose in tap water and a daily dose of chrysin (100 mg/kg) by oral administration (fructose + chrysin). All groups were fed ad libitum with standard laboratory chow diet and dietary manipulation lasted 18 weeks.
Fructose-feeding for 18 weeks induced an increase in serum triacylglycerols, insulin and angiotensin II levels and in hepatic fibrosis and these changes did not occur in fructose + chrysin rats. Moreover, the increase in both systolic and diastolic blood pressure which was found in fructose-fed animals from week 14th onwards was not observed in fructose + chrysin animals. In contrast, the increase in energy consumption, liver/body, heart/body and right kidney/body weight ratios, serum proteins, serum leptin and liver triacylglycerols observed in fructose-fed rats was not affected by chrysin.
Chrysin was able to protect against some of the MS features induced by fructose-feeding.
KeywordsMetabolic syndrome Chrysin Fructose Hypertension Triacylglycerol
Oral glucose tolerance test
Systolic blood pressure
Diastolic blood pressure
Mean arterial pressure
Very low-density lipoproteins
Homeostatic model assessment for insulin resistance
Messenger ribonucleic acid
Non-esterified fatty acids
Sterol regulatory binding protein 1c
Carbohydrate response element binding protein
Angiotensin type 1
Reactive oxygen species
Nicotinamide adenine dinucleotide phosphate reductase
We thank animal facility crew from Faculty of Medicine of the University of Porto for all technical support.
Conception and design: NA and FM. Acquisition of data: NA. Technical support: JTG, IR, LG. Analysis and interpretation of data: NA, FM, EK, SA, CS. Drafting the article and revising it for intellectual content: NA, FM. Study Supervision: FM. Final approval of the completed article: NA, FM, EK, SA, CS, JTG, IR, LG.
This work was financed by CAPES—Brazilian Federal Agency for Support and Evaluation of Graduate Education within the Ministry of Education of Brazil, for financing this project—PN: 10103/13-9.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
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