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Chronic consumption of the dietary polyphenol chrysin attenuates metabolic disease in fructose-fed rats

  • Nelson Andrade
  • Sara Andrade
  • Claúdia Silva
  • Ilda Rodrigues
  • Luísa Guardão
  • João T. Guimarães
  • Elisa Keating
  • Fátima Martel
Original Contribution
  • 28 Downloads

Abstract

Purpose

Metabolic syndrome (MS) is a major public health issue worldwide and fructose consumption has been associated with MS development. Recently, we showed that the dietary polyphenol chrysin is an effective inhibitor of fructose uptake by human intestinal epithelial cells. Therefore, our aim was to investigate if chrysin interferes with the development of MS induced by fructose in an animal model.

Methods

Adult male Sprague–Dawley rats (220–310 g) were randomly divided into four groups: (A) tap water (control), (B) tap water and a daily dose of chrysin (100 mg/kg) by oral administration (chrysin) (C) 10% fructose in tap water (fructose), and (D) 10% fructose in tap water and a daily dose of chrysin (100 mg/kg) by oral administration (fructose + chrysin). All groups were fed ad libitum with standard laboratory chow diet and dietary manipulation lasted 18 weeks.

Results

Fructose-feeding for 18 weeks induced an increase in serum triacylglycerols, insulin and angiotensin II levels and in hepatic fibrosis and these changes did not occur in fructose + chrysin rats. Moreover, the increase in both systolic and diastolic blood pressure which was found in fructose-fed animals from week 14th onwards was not observed in fructose + chrysin animals. In contrast, the increase in energy consumption, liver/body, heart/body and right kidney/body weight ratios, serum proteins, serum leptin and liver triacylglycerols observed in fructose-fed rats was not affected by chrysin.

Conclusions

Chrysin was able to protect against some of the MS features induced by fructose-feeding.

Keywords

Metabolic syndrome Chrysin Fructose Hypertension Triacylglycerol 

Abbreviations

MS

Metabolic syndrome

TAG

Triacylglycerol

FRUCT

Fructose

CHRYS

Chrysin

CV

Cardiovascular diseases

CONT

Control

OGTT

Oral glucose tolerance test

SBP

Systolic blood pressure

DBP

Diastolic blood pressure

MAP

Mean arterial pressure

HR

Heart rate

AST

Aspartate aminotransferase

ALT

Alanine aminotransferase

ALP

Alkaline phosphatase

VLDL

Very low-density lipoproteins

LDL

Low-density lipoprotein

HDL

high-density lipoproteins

CRP

C-reactive protein

HOMA-IR

Homeostatic model assessment for insulin resistance

mRNA

Messenger ribonucleic acid

NEFA

Non-esterified fatty acids

SREBP-1c

Sterol regulatory binding protein 1c

ChREBP

Carbohydrate response element binding protein

HMG-CoA

3-Hidroxi-3-methyl-glutaril-CoA reductase

NASH

Non-alcoholic steatohepatitis

AT1

Angiotensin type 1

NO

Nitric oxide

ROS

Reactive oxygen species

NADPH

Nicotinamide adenine dinucleotide phosphate reductase

Notes

Acknowledgements

We thank animal facility crew from Faculty of Medicine of the University of Porto for all technical support.

Author contributions

Conception and design: NA and FM. Acquisition of data: NA. Technical support: JTG, IR, LG. Analysis and interpretation of data: NA, FM, EK, SA, CS. Drafting the article and revising it for intellectual content: NA, FM. Study Supervision: FM. Final approval of the completed article: NA, FM, EK, SA, CS, JTG, IR, LG.

Funding

This work was financed by CAPES—Brazilian Federal Agency for Support and Evaluation of Graduate Education within the Ministry of Education of Brazil, for financing this project—PN: 10103/13-9.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Unit of Biochemistry, Department of Biomedicine, Faculty of Medicine of PortoUniversity of PortoPortoPortugal
  2. 2.Instituto de Investigação e Inovação em Saúde (I3S)University of PortoPortoPortugal
  3. 3.Department of Clinical PathologySão João Hospital CentrePortoPortugal
  4. 4.Institute of Public HealthUniversity of PortoPortoPortugal
  5. 5.CINTESIS, Center for Research in Health Technologies and Information SystemsUniversity of PortoPortoPortugal

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