Curcuma supplementation in high-fat-fed C57BL/6 mice: no beneficial effect on lipid and glucose profile or prevention of weight gain
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This experimental study investigated the effects of curcuma supplementation on weight gain, Body Adiposity Index, glucose and lipid profile, and liver and pancreas histology in C57BL/6 mice fed with a high-fat diet.
40 animals were separated into four groups: standard diet (SD), standard diet plus curcuma (SD + C), high-fat diet (HFD), and high-fat diet plus curcuma (HFD + C). Curcuma dose was 8 mg/animal/day. Histological and biochemical analyses were performed at the end of the experimental period.
Curcuma prevented weight gain, despite a higher food intake, and increased brown adipose tissue weight only in mice receiving standard diet. However, these changes were not observed in HFD + C group. The groups that received curcuma (SD + C and HFD + C) showed a pancreas with diffuse macro- and microgoticular steatosis.
Curcuma supplementation did not prevent weight gain or improved glucose and lipid profile in mice receiving high-fat diet. Furthermore, there was evidence of possible curcuma toxicity in the pancreas of C57BL/6 mice. The implications of these findings on humans still need to be investigated.
KeywordsCurcuma Weight gain Phytotherapy Toxicity C57BL/6 mice
Acyl-CoA cholesterol acyltransferase
Analysis of variance
Area under the curve
Brown adipose tissue
Body Mass Index
Enzyme linked immunonosorbent assay
Glucose tolerance test
High density lipoprotein cholesterol
High-fat diet plus curcuma
3-Hydroxy-3- methyl-glutaryl-coenzime A
High-performance liquid chromatography
Non-alcoholic fatty liver disease
Non-alcoholic fatty pancreas disease
Protein kinase B
Peroxisome proliferator-activated receptor
Reactive oxygen species
- SD + C
Standard diet plus curcuma
Sterol regulatory element-binding protein 1
Sterol regulatory element-binding protein 2
Total serum cholesterol
Vascular endothelial growth factor
Vascular endothelial growth receptor 2
We thank Andreia F. C. Leone Aguiar and Paula Payão Ovidio for assistance with biochemical and histological analyses.
The authors responsibilities were as follows: CBS, PGF, and VMMS: design, writing, and final content of the research; CBS: conducted research; LNZR, AJCMZ, ECC, and DC: provided essential materials and support; CBS, AJCMZ, and PGF: analysed data or performed statistical analysis; CBS, PGF, and VMMS: wrote paper; CBS and VMMS: had primary responsibility for the final content. All authors have read and approved the final manuscript.
This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant number 134187/2015-6).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- 1.World Health Organization (2018) Fact sheet no. 311: obesity and overweight. http://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight. Accessed 30 Nov 2018
- 2.Brasil. Ministério da Saúde. Vigitel Brasil 2017. http://bvsms.saude.gov.br/bvs/publicacoes/vigitel_brasil_2017_vigilancia_fatores_riscos.pdf. Accessed 30 Nov 2018
- 3.Ng M, Fleming T, Robinson M, Thomson B, Graetz N, Margono C, Mullany EC, Biryukov S, Abbafati C, Abera SF et al (2014) Global, regional, and national prevalence of overweight and obesity in children and adults during 1980–2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet 384:766–781CrossRefGoogle Scholar
- 9.Singh G, Kapoor IPS, Pandey SK, Singh OP (2003) Curcuma longa—chemical, antifungal and antibacterial investigation of rhizome oil. Indian Perfum 47(2):173–178Google Scholar
- 23.Nishikawa S, Kamiya M, Aoyama H, Nomura M, Hyodo T, Ozeki A, Lee H, Takahashi T, Imaizumi A, Tsuda T (2018) Highly dispersible and bioavailable curcumin but not native curcumin induces brown-like adipocyte formation in mice. Mol Nutr Food Res 62(5)Google Scholar
- 32.NTP Toxicology and Carcinogenesis Studies of Curcuma Oleoresin (1993) (CAS no. 8024-37-1) (major component 79%–85% curcumin, CAS no. 458-37-7) in F344/N rats and B6C3F1 mice (feed studies). Natl Toxicol Program Tech Rep Ser 427:1–275Google Scholar