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Curcuma supplementation in high-fat-fed C57BL/6 mice: no beneficial effect on lipid and glucose profile or prevention of weight gain

  • Caroline Bertoncini SilvaEmail author
  • Priscila Giacomo Fassini
  • Leandra Náira Zambelli Ramalho
  • Edemilson Cardoso da Conceição
  • Aline José Coelho Moreira Zordan
  • Daniela Carlos
  • Vivian Marques Miguel Suen
Original Contribution
  • 61 Downloads

Abstract

Purpose

This experimental study investigated the effects of curcuma supplementation on weight gain, Body Adiposity Index, glucose and lipid profile, and liver and pancreas histology in C57BL/6 mice fed with a high-fat diet.

Methods

40 animals were separated into four groups: standard diet (SD), standard diet plus curcuma (SD + C), high-fat diet (HFD), and high-fat diet plus curcuma (HFD + C). Curcuma dose was 8 mg/animal/day. Histological and biochemical analyses were performed at the end of the experimental period.

Results

Curcuma prevented weight gain, despite a higher food intake, and increased brown adipose tissue weight only in mice receiving standard diet. However, these changes were not observed in HFD + C group. The groups that received curcuma (SD + C and HFD + C) showed a pancreas with diffuse macro- and microgoticular steatosis.

Conclusions

Curcuma supplementation did not prevent weight gain or improved glucose and lipid profile in mice receiving high-fat diet. Furthermore, there was evidence of possible curcuma toxicity in the pancreas of C57BL/6 mice. The implications of these findings on humans still need to be investigated.

Keywords

Curcuma Weight gain Phytotherapy Toxicity C57BL/6 mice 

Abbreviations

ACAT

Acyl-CoA cholesterol acyltransferase

ANOVA

Analysis of variance

AUC

Area under the curve

BAT

Brown adipose tissue

BHT

Butylated hydroxytoluene

BMI

Body Mass Index

DNA

Deoxyribonucleic acid

ELISA

Enzyme linked immunonosorbent assay

GLP-1

Glucagon-like peptide-1

GTT

Glucose tolerance test

HDL-c

High density lipoprotein cholesterol

HF

High-fat

HFD

High-fat diet

HFD+C

High-fat diet plus curcuma

HMG-CoA

3-Hydroxy-3- methyl-glutaryl-coenzime A

HPFs

High-power fields

HPLC

High-performance liquid chromatography

LDL

Low-density lipoproteins

NAFLD

Non-alcoholic fatty liver disease

NAFPD

Non-alcoholic fatty pancreas disease

PKB

Protein kinase B

PPAR

Peroxisome proliferator-activated receptor

ROS

Reactive oxygen species

SD

Standard diet

SD + C

Standard diet plus curcuma

SREBP1

Sterol regulatory element-binding protein 1

SREBP2

Sterol regulatory element-binding protein 2

TC

Total serum cholesterol

UCP-1

Uncoupling protein-1

VEGF

Vascular endothelial growth factor

VEGF-R2

Vascular endothelial growth receptor 2

Notes

Acknowledgements

We thank Andreia F. C. Leone Aguiar and Paula Payão Ovidio for assistance with biochemical and histological analyses.

Author contributions

The authors responsibilities were as follows: CBS, PGF, and VMMS: design, writing, and final content of the research; CBS: conducted research; LNZR, AJCMZ, ECC, and DC: provided essential materials and support; CBS, AJCMZ, and PGF: analysed data or performed statistical analysis; CBS, PGF, and VMMS: wrote paper; CBS and VMMS: had primary responsibility for the final content. All authors have read and approved the final manuscript.

Funding

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant number 134187/2015-6).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Caroline Bertoncini Silva
    • 1
    Email author
  • Priscila Giacomo Fassini
    • 1
  • Leandra Náira Zambelli Ramalho
    • 2
  • Edemilson Cardoso da Conceição
    • 3
  • Aline José Coelho Moreira Zordan
    • 1
  • Daniela Carlos
    • 4
  • Vivian Marques Miguel Suen
    • 1
  1. 1.Division of Nutrology, Department of Internal Medicine, Ribeirão Preto Medical SchoolUniversity of São PauloRibeirão PretoBrazil
  2. 2.Departament of Pathology, Ribeirão Preto Medical SchoolUniversity of São PauloRibeirão PretoBrazil
  3. 3.Faculty of Pharmacy of Federal University of GoiásGoiâniaBrazil
  4. 4.Department of Biochemistry and Immunology, Ribeirão Preto Medical SchoolUniversity of São PauloRibeirão PretoBrazil

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