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European Journal of Nutrition

, Volume 58, Issue 2, pp 653–663 | Cite as

Xanthohumol exerts protective effects in liver alterations associated with aging

  • Cristina Fernández-García
  • Lisa RancanEmail author
  • Sergio D. Paredes
  • César Montero
  • Mónica de la Fuente
  • Elena Vara
  • Jesús A. F. Tresguerres
Original Contribution

Abstract

Background and aims

Aging is associated with a deregulation of biological systems that lead to an increase in oxidative stress, inflammation, and apoptosis, among other effects. Xanthohumol is the main preylated chalcone present in hops (Humulus lupulus L.) whose antioxidant, anti-inflammatory and chemopreventive properties have been shown in recent years. In the present study, the possible protective effects of xanthohumol on liver alterations associated with aging were evaluated.

Methods

Male young and old senescence-accelerated prone mice (SAMP8), aged 2 and 10 months, respectively, were divided into four groups: non-treated young, non-treated old, old treated with 1 mg/kg/day xanthohumol, and old treated with 5 mg/kg/day xanthohumol. Male senescence-accelerated resistant mice (SAMR1) were used as controls. After 30 days of treatment, animals were sacrificed and livers were collected. mRNA (AIF, BAD, BAX, Bcl-2, eNOS, HO-1, IL-1β, NF-κB2, PCNA, sirtuin 1 and TNF-α) and protein expressions (BAD, BAX, AIF, caspase-3, Blc-2, eNOS, iNOS, TNF-α, IL1β, NF-κB2, and IL10) were measured by RT-PCR and Western blotting, respectively. Mean values were analyzed using ANOVA.

Results

A significant increase in mRNA and protein levels of oxidative stress, pro-inflammatory and proliferative markers, as well as pro-apoptotic parameters was shown in old non-treated SAMP8 mice compared to the young SAMP8 group and SAMR1 mice. In general, age-related oxidative stress, inflammation and apoptosis were significantly decreased (p < 0.05) after XN treatment. In most cases, this effect was dose-dependent.

Conclusions

XN was shown to modulate inflammation, apoptosis, and oxidative stress in aged livers, exerting a protective effect in hepatic alterations.

Keywords

Liver Aging Inflammation Senescence-accelerated mouse Xanthohumol 

Notes

Acknowledgements

The authors would like to thank Medicine students Paula Corral, Bryan Hyacinthe, and Mario Calvo-Soto (School of Medicine, Complutense University of Madrid, Spain) for their continued interest and cooperation in our work. The skillful technical assistance of Rocío Campón (Dept. of Physiology, School of Medicine, Complutense University of Madrid, Spain) is also gratefully acknowledged.

Author contributions

EV, JAFT designed the research; CFG, LR, SDP, CM conducted the research; EV, MF analyzed the data; CFG, LR, SDP, EV wrote the paper. All authors read and approved the final manuscript.

Funding

This study was supported by Grants from Red de Fragilidad y Envejecimiento RETICEF (RD12/0043/0032) and GRUPOS UCM-BSCH (GR35/10-A).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Cristina Fernández-García
    • 1
  • Lisa Rancan
    • 1
    Email author
  • Sergio D. Paredes
    • 2
  • César Montero
    • 1
  • Mónica de la Fuente
    • 3
  • Elena Vara
    • 1
  • Jesús A. F. Tresguerres
    • 2
  1. 1.Department of Biochemistry and Molecular Biology, School of MedicineUniversity Complutense of MadridMadridSpain
  2. 2.Department Physiology, School of MedicineComplutense University of MadridMadridSpain
  3. 3.Department of Physiology, Genetics and Microbiology, School of BiologyComplutense University of MadridMadridSpain

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