Diet supplemented with phytochemical epigallocatechin gallate and probiotic Lactobacillus fermentum confers second generation synbiotic effects by modulating cellular immune responses and antioxidant capacity in aging mice
In the present study, we systematically identified and evaluated a synbiotic combination of phytochemical epigallocatechin gallate (EGCG) and probiotic bacteria in amelioration of immunosenescence and oxidative stress in aged mice.
Inhibitory effects of EGCG against different bacterial species were evaluated in vitro, followed by analysis to identify potential combination of EGCG and probiotic bacteria against alleviation of oxidative and inflammatory stress ex vivo. The best synbiotic combination, vis-à-vis prebiotic and probiotic supplementation alone, was then evaluated in aged Swiss albino mice for modulation of various immunological and antioxidative parameters.
EGCG strongly inhibited the growth of pathogenic microbes as compared to probiotic bacteria. A combination of EGCG with probiotic Lactobacillus fermentum (LF) provided evidence of additive effects in the amelioration of oxidative and inflammatory stress-induced cell death. In vivo study revealed that combined supplementation of LF and EGCG significantly enhanced neutrophil oxidative index, CD3+ cell numbers and activation status, Th1/Th2 cytokines in splenic supernatants as well as liver Nrf-2 expression in comparison with treatments with LF or EGCG alone. The combined application of EGCG and LF did not simply result in additive or synergistic effects in relation with individual treatments.
These observations suggest that EGCG could be considered as a potential prebiotic that can offer second generation synbiotic health beneficial effects for the alleviation of some of the deleterious aspects of immunosenescence and aging.
KeywordsEGCG Probiotics Prebiotics Synbiotics Immunity Aging
Peripheral blood mononuclear cells
Nuclear factor kappa-light-chain enhancer of activated B cells
Authors acknowledge the Director, CSIR-IHBT, Palampur for supporting and providing all the necessary facilities. This work was supported by grants from the Science and Engineering Research Board, Government of India, through the Young Scientist Scheme (YSS/2015/000758) and MLP0204 of CSIR Government of India.
Compliance with ethical standards
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- 5.Vodnar DC, Socaciu C (2012) Green tea increases the survival yield of Bifidobacteria in simulated gastrointestinal environment and during refrigerated conditions. Chem Cent J 6(1):61Google Scholar
- 6.Ampatzoglou A, Atwal KK, Maidens CM, Williams CL, Ross AB, Thielecke F, Jonnalagadda SS, Kennedy OB, Yaqoob P (2015) Increased whole grain consumption does not affect blood biochemistry, body composition, or gut microbiology in healthy, low-habitual whole grain consumers. J Nutr 145(2):215–221CrossRefGoogle Scholar
- 14.Kumari M, Swarnkar MK, Kumar S, Singh AK, Gupta M (2015) Genome sequence of a potential probiotic strain, Lactobacillus fermentum HFB3, isolated from a human Gut. Genome Announc 3(6):e01296–e01315Google Scholar
- 15.Kumari M, Swarnkar MK, Kumar S, Singh AK, Gupta M (2015) Complete genome sequence of potential probiotic Lactobacillus sp. HFC8, isolated from human gut using PacBio SMRT sequencing. Genome Announc 3(6):e01315–e01337Google Scholar
- 20.Mair KH, Stadler M, Talker SC, Forberg H, Storset AK, Müllebner A, Duvigneau JC, Hammer SE, Saalmuller A, Gerner W (2016) Porcine CD3+NKp46+ lymphocytes have NK-cell characteristics and are present in increased frequencies in the lungs of influenza-infected animals. Front Immun 7:263CrossRefGoogle Scholar
- 35.Le Garff-Tavernier M, Béziat V, Decocq J, Siguret V, Gandjbakhch F, Pautas E, Debré P, Merle-Beral H, Vieillard V (2010) Human NK cells display major phenotypic and functional changes over the life span. Aging Cell 9(4):527–535. https://doi.org/10.1111/j.1474-9726.2010.00584.x CrossRefGoogle Scholar
- 41.Tilstra JS, Clauson CL, Niedernhofer LJ, Robbins PD (2011) NF-κB in aging and disease. Aging Dis 2(6):449–465Google Scholar
- 46.Seenappanahalli Nanjundaiah Y, Wright DA, Baydoun AR, O’Hare WT, Ali Z, Khaled Z, Sarker MH (2016) Lactobacillus rhamnosus GG conditioned media modulates acute reactive oxygen species and nitric oxide in J774 murine macrophages. Biochem Biophys Rep 7:6:68–75Google Scholar