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The importance of volume and area fractions of cerebellar volume and vermian subregion areas: a stereological study on MR images

  • Fatma Dilek Taman
  • Piraye Kervancioglu
  • Ahmet Selim Kervancioglu
  • Begümhan TurhanEmail author
Original Article
  • 37 Downloads

Abstract

Purpose

Age, gender, and body size are important factors which are affecting the cerebellar volume (CV). Many neurological diseases lead changes in CV. The aim of this study is to measure CV and the total intracranial volume (TIV) for both genders on magnetic resonance images (MRI), to calculate the CV/TIV volume fraction, and also to determine the normal values that can be regarded clinically significant by determining the total vermis area and vermian subregion areas (V1, V2, and V3).

Methods

In this retrospective study, MR images (without any pathological findings) of 200 individuals (100 female, 100 male) between the ages of 20–40 were used. CV and CV/TIV volume fractions, vermian subregion areas, and area fractions were calculated by using the Stereoinvestigator 8.0 (Microbrightfield, USA) software. The volumetric calculations were performed by the point counting method according to the Cavalieri principle, which is one of the volume calculation methods in stereology. Total CV, TIV, cerebellar vermis areas (V1, V2, and V3), and total cerebellum area were measured separately for both groups.

Results

The volume of cerebellum was 120.53 ± 11.1 cm3 in males, 105.99 ± 11.2 cm3 in females, TIV was 1304.99 ± 91.7 cm3 in males and 1155.15 ± 85.7 cm3 in females. CV and TIV were statistically higher in males (p = 0.001, p = 0.001 respectively). It was observed that the differences between the genders in terms of CV/TIV disappeared (p = 0.679). The total vermis area was 11.59 ± 1.3 cm2 in males and 10.85 ± 1.3 cm2 in females. V1 area, V3 area, and the total vermis area were found statistically higher in males (p = 0.05, p = 0.006, p = 0.007 respectively). It was determined that the area fraction of V2 was higher in females when the fractions of V1, V2, and V3 to the total vermis area were examined (p = 0.03).

Conclusion

We believe that the normal values of CV, TIV, and vermian subregion areas, determined by stereological method, will contribute to the diagnosis and the treatment plan of the clinical pathological evaluations in adults and children.

Keywords

Cavalieri principle Cerebellum volume Stereology Cerebellar vermis area 

Notes

Author contribution

P Kervancioglu, S Kervancioglu, FD Taman, B Turhan: Project development

S Kervancioglu, FD Taman, P Kervancioglu: Data collection, data analysis

S Kervancioglu: Data management

P Kervancioglu, B Turhan, FD Taman: Manuscript writing/editing

Compliance with ethical standards

Conflict of interest

The authors of this paper have no conflicts of interest, including specific financial interests, relationships, and/or affiliations relevant to the subject matter or materials included.

Ethical approval

All procedures performed in the study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

References

  1. 1.
    Allen JS, Damasio H, Grabowski TJ (2002) Normal neuroanatomical variation in the human brain: an MRI-volumetric study. Am J Phys Anthropol 118:341–358CrossRefGoogle Scholar
  2. 2.
    Bottmer C, Bachmann S, Pantel J, Essig M, Amann M, Schad LR, Schröder J (2005) Reduced cerebellar volume and neurological soft signs in first-episode schizophrenia. Psychiatry Res 140:239–250CrossRefGoogle Scholar
  3. 3.
    Karabekir HS, Mas NG, Yilmaz ÖK, Baş O, Ertekin T, Yazıcı AC, Senan S (2009) Evaluation of cerebellar asymmetry with vertigo cases: a stereological study. Turk Neurosurg 19(1):15–20Google Scholar
  4. 4.
    Raz N (2000) Aging of the brain and its impact on cognitive performance: integration of structural and functional findings. In: Craik FIM, Salthouse TA (eds) The handbook of aging and cognition. Lawrence Erlbaum Associates Publishers, Mahwah, pp 1–90Google Scholar
  5. 5.
    Rhyu IJ, Cho TH, Lee NJ, Uhm C-S, Kim H, Suh Y-S (1999) Magnetic reasonance image based cerebellar volumetry in healthy Korean adults. Neurosci Lett 270:149–152CrossRefGoogle Scholar
  6. 6.
    Raz N, Dupuis JH, Briggs SD, McGavran C, Acker JD (1998) Differential effects of age and sex on the cerebellar hemispheres and the vermis: a prospective MR study. AJNR Am J Neuroradiol 19:65–71Google Scholar
  7. 7.
    Anderson CM, Maas LC, Frederick BB, Bendor JT, Spencer T, Livni E, Lukas SE, Fischman AJ, Madras BK, Renshaw PF, Kaufman MJ (2006) Cerebellar vermis involvement in cocaine-related behaviors. Neuropsychopharmacology 31:1318–1326CrossRefGoogle Scholar
  8. 8.
    MacLullich AM, Edmond CL, Ferguson KJ, Wardlaw JM, Starr JM, Seckl JR, Deary IJ (2004) Size of the neocerebellar vermis is associated with cognition in healthy elderly men. Brain Cogn 56(3):344–348CrossRefGoogle Scholar
  9. 9.
    Mills NP, Delbello MP, Adler CM, Strakowski SM (2005) MRI analysis of cerebellar vermal abnormalities in bipolar disorder. Am J Psychiatry 162:1530–1532CrossRefGoogle Scholar
  10. 10.
    Chung SC, Lee BY, Tack GR, Lee SY, Eom JS, Sohn JH (2005) Effects of age, gender, and weight on the cerebellar volume of Korean people. Brain Res 1042:233–235CrossRefGoogle Scholar
  11. 11.
    Murshed KA, Ziylan T, Seker M, Cicekcibasi AE, Acikgozoglu S (2003) Morphometric assessment of brain stem and cerebellar vermis with midsagittal MRI: the gender differences and effects of age. Neuroanatomy 2:35–38Google Scholar
  12. 12.
    Escalona PR, McDonald WM, Doraiswamy PM, Boyko OB, Husain MM, Figiel GS, Laskowitz D, Ellinwood KR Jr (1991) In vivo stereological assessment of human cerebellar volume: effects of gender and age. AJNR Am J Neuroradiol 12:927–929Google Scholar
  13. 13.
    Luft AR, Skalej M, Schultz JB, Welte D, Kolb R, Bürk K, Klockgether T, Voigt K (1999) Patterns of age-related shrinkage in the cerebellum and brainstem observed in vivo using three-dimensional MRI volumetry. Cereb Cortex 9:712–721CrossRefGoogle Scholar
  14. 14.
    Acer N, Sahin B, Usanmaz M, Tatoğlu H, Irmak Z (2008) Comparison of point counting and planimetry methods for the assessment of cerebellar volume in human using magnetic resonance imaging: a stereological study. Surg Radiol Anat 30:335–339CrossRefGoogle Scholar
  15. 15.
    Ekinci N, Acer N, Akkaya A, Sankur S, Kabadayı T, Sahin B (2008) Volumetric evaluation of the relations among the cerebrum, cerebellum and brain stem in young subjects: a combination of stereology and magnetic resonance imaging. Surg Radiol Anat 30:489–494CrossRefGoogle Scholar
  16. 16.
    Mazonakis M, Karampekios S, Damilakis J, Voloudaki A, Gourtsoyiannis N (2004) Stereological estimation of total intracranial volume on CT images. Eur Radiol 14:1285–1290CrossRefGoogle Scholar
  17. 17.
    Odacı E, Bahadır A, Yıldırım S, Sahin B, Canan S, Bas O, Bilgic S, Kaplan S (2005) Volume estimation using the Cavalieri principle on computerized tomography and magnetic resonance images and its clinical application: review. Turkiye Klinikleri J Med Sci 25:421–428Google Scholar
  18. 18.
    De Bellis MD, Keshavan MS, Beers SR, Hall J, Frustaci K, Masalehdan A, Noll J, Boring AM (2001) Sex differences in brain maturation during childhood and adolescence. Cereb Cortex 11:552–557CrossRefGoogle Scholar
  19. 19.
    Manjunath KY (2002) Estimation of cranial volume-an overview of methodologies. J Anat Soc India 51:85–91Google Scholar
  20. 20.
    Acer N, Sahin B, Bas O, Ertekin T, Usanmaz M (2007) Comparison of three methods for the estimation of total intracranial volume: stereologic, planimetric, and anthropometric approaches. Ann Plast Surg 58:48–53CrossRefGoogle Scholar
  21. 21.
    Kruggel F (2006) MRI based volumetry of head compartments: normative values of healthy adults. Neuroimage 30:1–11CrossRefGoogle Scholar
  22. 22.
    Sahin B, Acer N, Sonmez OF, Emirzeoglu M, Basaloglu H, Uzun A, Bilgic S (2007) Comparison of four methods for the estimation of intracranial volume: a gold standard study. Clin Anat 20:766–773CrossRefGoogle Scholar
  23. 23.
    Manjunath KY (2002) Estimation of cranial volume in dissecting room cadavers. J Anat Soc India 51:168–172Google Scholar
  24. 24.
    Matsumae M, Kikinis R, Morocz IA, Lorenzo AV, Sandor T, Albert MS, Black PM, Jolesz FA (1996) Age-related changes in intracranial compartment volumes in normal adults assessed by magnetic resonance imaging. J Neurosurg 84:982–991CrossRefGoogle Scholar
  25. 25.
    Supprian T, Ulmar G, Bauer M, Schüler M, Püschel K, Retz-Junginger P, Schmitt HP, Heinsen H (2000) Cerebellar vermis area in schizophrenic patients — a post-mortem study. Schizophr Res 42:19–28CrossRefGoogle Scholar
  26. 26.
    Oguro H, Okada K, Yamaguchi S, Kobayashi S (1998) Sex differences in morphology of the brain stem and cerebellum with normal ageing. Neuroradiology 40:788–792CrossRefGoogle Scholar
  27. 27.
    Quattrone A, Cerasa A, Messina D, Nicoletti G, Hagberg GE, Lemieux L, Novellino F, Lanza P, Arabia G, Salsone M (2008) Essential head tremor is associated with cerebellar vermis atrophy: a volumetric and voxel-based morphometry MR imaging study. AJNR Am J Neuroradiol 29:1692–1697CrossRefGoogle Scholar
  28. 28.
    Ciesielski KT, Yanofsky R, Ludwig RN, Hill DE, Hart BL, Astur RS, Snyder T (1994) Hypoplasia of the cerebellar vermis and cognitive deficits in survivors of childhood leukemia. Arch Neurol 51:985–993CrossRefGoogle Scholar
  29. 29.
    Lawson JA, Vogrin S, Bleasel AF, Cook MJ, Bye AM (2000) Cerebral and cerebellar volume reduction in children with intractable epilepsy. Epilepsia 41:1456–1462CrossRefGoogle Scholar
  30. 30.
    Monkul ES, Hatch JP, Sassi RB, Axelson D, Brambilla P, Nicoletti MA, Keshavan MS, Ryan ND, Birmaher B, Soares JC (2008) MRI study of the cerebellum in young bipolar patients. Prog Neuro-Psychopharmacol Biol Psychiatry 32:613–619CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Active Healthy Life CenterInstitute of Applied OsteopathyIstanbulTurkey
  2. 2.Department of Anatomy, Faculty of MedicineGaziantep UniversityGaziantepTurkey
  3. 3.Department of Radiology, Faculty of MedicineSanko UniversityGaziantepTurkey
  4. 4.Department of Physical Therapy and Rehabilitation, Faculty of Health SciencesHasan Kalyoncu UniversityGaziantepTurkey

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