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Tumor necrosis factor alpha and interleukin-1 beta levels in cerebrospinal fluid examination for the diagnosis of ventriculoperitoneal shunt-related ventriculitis

  • Semih K. OlgunerEmail author
  • Bulent Boyar
  • Derya Alabaz
  • Tahsin Erman
  • Kadir Oktay
  • Ali Arslan
  • Emre Bilgin
  • Ali Ihsan Okten
Original Article
  • 21 Downloads

Abstract

Purpose

Ventriculitis is known to develop after chronic inflammation and bacterial invasion of the ventricular surface with a recurrence of shunt infections. The aim of this study is to evaluate the diagnostic value of elevation in cerebrospinal fluid (CSF) interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) together with CSF culture and laboratory test results in the diagnosis of ventriculoperitoneal (VP) shunt-related ventriculitis, which is known to be more problematic than conventional shunt infection.

Methods

The study included a total of 34 patients with a VP shunt due to hydrocephalus, who presented with a headache, fever, and shunt infection at the Emergency Department and had a pre-diagnosis of ventriculitis. Nineteen patients were diagnosed with shunt-related infection or ventriculitis using the CSF obtained from the shunt pump. The IL-1β and TNF-α levels from the CSF samples of all patients were measured using the Micro ELISA immunoassay method.

Results

CSF direct microscopic observation revealed that the mean cell count, IL-1β level, CRP level, and blood leukocyte level were higher in patients with ventriculitis compared to those diagnosed with shunt infection (p = 0.02, p = 0.009, p = 0.004, and p = 0.009, respectively). The probability of predicting positive culture outcome was 92.7% with 90.9% sensitivity and 82.6% specificity when IL-1β values exceeded 4.0 pg/ml. TNF-α values did not show a significant, reliable pattern compared to IL-1β.

Conclusions

IL-1β is a reliable parameter which shall be used in the diagnosis of ventriculitis by predicting positive culture outcome with high sensitivity and specificity.

Keywords

Shunt infections Ventriculitis TNF-α IL-1β 

Notes

Authors’ contributions

Semih K. Olguner, Bulent Boyar, Derya Alabaz, and Tahsin Erman were involved in the study conception and design. Ali İhsan Okten, Ali Arslan, Emre Bilgin, and Kadir Oktay were responsible for the data analysis. All authors contributed to the discussion, interpreted the findings, helped write, reviewed/edited the manuscript for intellectual content, and read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval and consent to participate

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethics Committee of Çukurova Medical Faculty. All participants provided informed consent.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of NeurosurgeryAdana City Training Research HospitalAdanaTurkey
  2. 2.Department of NeurosurgeryCukurova University of Medical SchoolAdanaTurkey
  3. 3.Pediatric Infectious Disease DepartmentCukurova University of Medical SchoolAdanaTurkey
  4. 4.Department of NeurosurgeryMedical Park HospitalGaziantepTurkey

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