Child's Nervous System

, Volume 34, Issue 8, pp 1479–1487 | Cite as

Effect evaluation of methylprednisolone plus mitochondrial division inhibitor-1 on spinal cord injury rats

  • Xu-Gui Chen
  • Li-Hua Chen
  • Ru-Xiang Xu
  • Hong-Tian ZhangEmail author
Original Paper



To investigate the combination effect of methylprednisolone (MP) and mitochondrial division inhibitor-1 (Mdivi-1) on the neurological function recovery of rat spinal cord injury (SCI) model.


The weight-drop method was used to establish the rat SCI model; then, rats were randomized into sham group, SCI group, MP group, Mdivi-1 group and MP+Mdivi-1 group. Motor function scores were quantified to evaluate locomotor ability; HE staining was used to assess spinal cord histopathology; tissue water content, oxidative stress, tissue mitochondrial function, neurons apoptosis, and apoptosis-related protein expression were detected.


From the third day after SCI, BBB score of the MP+Mdivi-1 group was obviously higher than the other experimental groups (p < 0.05). Compared with the SCI group, tissue water content of the Mdivi-1 group and MP+Mdivi-1 group reduced obviously (p < 0.05), mitochondrial membrane potential (MMP) level and ATP content in the Mdivi-1 group and MP+Mdivi-1 group were both higher (p < 0.05). Meanwhile, three kinds of treatment all reduced apoptosis significantly, while MP plus Mdivi-1 exhibited the best inhibition effect on apoptosis (p < 0.05). The expression levels of Drp1, cytochrome c, and caspase-3 were all upregulated obviously; Mdivi-1 could inhibit Drp1 upregulation induced by SCI; for the upregulation of cytochrome c and caspase-3, the inhibition effect of Mdivi-1 approached MP. When MP combined with Mdivi-1, there was the best inhibition effect.


MP combined with Mdivi-1 may produce better neurological function recovery, through improving functional status of mitochondria and inhibiting lipid peroxidation in damaged tissue of SCI rats, and thus alleviating apoptosis.


Mitochondrial division inhibitor-1 Methylprednisolone Neurological function Spinal cord injury 


Funding information

This research was supported by the National Natural Science Fund of China (81371345).

Compliance with ethical standards

Conflict of interest

On behalf of all authors, we state that there is no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xu-Gui Chen
    • 1
  • Li-Hua Chen
    • 1
  • Ru-Xiang Xu
    • 1
  • Hong-Tian Zhang
    • 1
    Email author
  1. 1.The Affiliated Bayi Brain Hospitalthe Army General Hospital PLABeijingChina

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