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Antioxidative enzymes in irradiated rat brain—indicators of different regional radiosensitivity

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Abstract

Purpose

Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of γ-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression.

Methods

Heads of 4-day-old female rats were irradiated with γ-rays, using 60Co. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis.

Results

One hour after exposure, γ-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of γ-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT.

Conclusions

Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.

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Acknowledgments

This work was financially supported by the Ministry of Education, Science and Technological Development (Grants 41027 and 41022).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Molecular Biology and Endocrinology, Vinča Institute of Nuclear Sciences, University of Belgrade, PO Box 522, 11001, Belgrade, Serbia

    Ana Todorović, Snežana Pejić, Vesna Stojiljković, Ljubica Gavrilović, Nataša Popović, Ivan Pavlović & Snežana B. Pajović

  2. Department of Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11060, Belgrade, Serbia

    Zorica S. Saičić

Authors
  1. Ana Todorović
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  2. Snežana Pejić
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  4. Ljubica Gavrilović
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  5. Nataša Popović
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  8. Snežana B. Pajović
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Correspondence to Ana Todorović.

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Todorović, A., Pejić, S., Stojiljković, V. et al. Antioxidative enzymes in irradiated rat brain—indicators of different regional radiosensitivity. Childs Nerv Syst 31, 2249–2256 (2015). https://doi.org/10.1007/s00381-015-2807-2

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  • DOI: https://doi.org/10.1007/s00381-015-2807-2

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