Community shift of microbial ammonia oxidizers in air-dried rice soils after 22 years of nitrogen fertilization
- 288 Downloads
In this study, we show that composition shifts of ammonia oxidizer communities imposed by a 22-year field fertilization regime could be well retained in both fresh and air-dried soils. The abundance and composition of ammonia-oxidizing bacteria (AOB) and archaea (AOA) were measured in fresh soils, which received no fertilization (CK), chemical fertilization (NPK), and chemical plus organic matter fertilization (NPK/OM) for 22 years. The air-drying treatment of fresh soil was also conducted for pairwise analysis. We found that in fresh soils DGGE fingerprints of AOB showed significant changes under both NPK and NPK/OM treatments when compared with control (CK) and that microbial shift was almost identical in air-dried soils. Long-term nutrient fertilization did not affect AOA communities in either air-dried or fresh soils. Compared to CK treatment, real-time PCR indicated that AOB amoA genes increased significantly in fresh soils of NPK (59-fold) and NPK/OM (48-fold) plots and in air-dried NPK and NPK/OM soils by 22-fold and 19-fold respectively. Our results demonstrate that community shifts of AOB in fresh soils under chronic N fertilization could be well preserved in air-dried soils, despite the apparent decline in absolute abundance of ammonia oxidizers. These results suggest that air-dried soil could be a useful resource for deciphering the adaptive strategy of ammonia oxidizers under N enrichment when the significant changes of community composition occurred in fresh soils.
KeywordsAir-dried soil Fresh soil Long-term fertilization Ammonia oxidizers
We thank Prof. Penny Hirsch for constructive comments and suggestions. The authors also want to thank Dr. Yucheng WU, Xue ZHOU, and Baozhan WANG for technical support and the members of our lab for helpful discussion.
This study was financially supported from the National Natural Science Foundation of China (41530857, 41501267, 41471208); the State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences (Y20160025); and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB15040000).
Compliance with ethical standards
The authors declare that they have no competing interests
- Chu HY, Lin XG, Fujii T, Morimoto S, Yagi K, Hu JL, Zhang JB (2007) Soil microbial biomass, dehydrogenase activity, bacterial community structure in response to long-term fertilizer management. Soil Biol Biochem 39:2971–2976Google Scholar
- Dolfing J, Feng YZ (2015) The importance of soil archives for microbial ecology. Nat Rev Microbiol 13:1Google Scholar
- Feng YZ, Chen RR, Hu JL, Zhao F, Wang JH, Chu HY, Zhang JB, Dolfing J, Lin XG (2015) Bacillus asahii comes to the fore in organic manure fertilized alkaline soils. Soil Biol Biochem 81:186–194Google Scholar
- Koops HP, Purkhold U, Pommerening-Roser A, Timmermann G, Wagner M (2006) The lithoautotrophic ammonia-oxidizing bacteria. Prokaryotes 778–811Google Scholar
- Liu R, Hayden HL, Suter H, Hu HW, Lam SK, He JZ, Mele PM, Chen DL (2017) The effect of temperature and moisture on the source of N2O and contributions from ammonia oxidizers in an agricultural soil. Biol Fertil Soils 53:141–152Google Scholar
- Potts M (1994) Desiccation tolerance of prokaryotes. Microbiol Rev 58:755–805Google Scholar
- Rotthauwe J, Witzel K, Liesack W (1997) The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl Environ Microbiol 63:4704–4712Google Scholar
- Wu YC, Lu L, Wang BZ, Lin XG, Zhu JG, Cai ZC, Yan XY, Jia ZJ (2011) Long-term field fertilization significantly alters community structure of ammonia-oxidizing bacteria rather than archaea in a paddy soil. Soil Sci Soc Am J 75:1431–1439Google Scholar
- Zornoza R, Guerrero C, Mataix-Solera J, Arcenegui V, Garcia-Renes F, Mataix-Beneyto J (2007) Assessing the effects of air-drying and rewetting pre-treatment on soil microbial biomass, basal respiration, metabolic quotient and soluble carbon under Mediterranean conditions. Eur J Soil Biol 43:120–129CrossRefGoogle Scholar