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Biology and Fertility of Soils

, Volume 55, Issue 2, pp 149–169 | Cite as

Streptomyces pactum Act12 controls tomato yellow leaf curl virus disease and alters rhizosphere microbial communities

  • Yulong Li
  • Qiao Guo
  • Yunzhou Li
  • Yifan Sun
  • Quanhong XueEmail author
  • Hangxian LaiEmail author
Original Paper
  • 166 Downloads

Abstract

Actinomycetes comprise a large group of biocontrol bacteria, yet no studies have reported on their effects against plant virus diseases. Here, we evaluated the control effects and the possible mechanisms of Streptomyces pactum Act12 against tomato yellow leaf curl virus disease (TYLCD) through field and pot experiments. We also analyzed changes in plant growth and rhizosphere microbial community composition following the application of Act12. The pre-inoculation of soil with the Act12 agent enhanced the activities of peroxidase and chitinase while upregulating the expression of genes related to plant systemic resistance (PR-1 and SIPI-II) and specific TYLCD resistance (SIPer1 and SIVRSLip) in tomato leaves under field conditions. The effects of Act12 at inducing a decline in TYLCD severity and promoting plant growth were deemed satisfactory in all three field experiments. In the pot experiments, Act12 induced a decline in the viral DNA level and an enhancing of peroxidase, polyphenoloxidase, and phenylalanine ammonia lyase activities in tomato leaves. Additionally, Act12 treatment reduced the accumulation of H2O2 and O·−2, increased the levels of salicylic and jasmonic acids, and upregulated the expression of genes related to plant resistance and RNA interference in tomato leaves. Applying Act12 also increased the diversity of bacteria and the abundance of potential beneficial microbes (e.g., Trichoderma and Bacillus) in the root-zone and root-surface soils, resulting in modular differentiation of co-occurrence networks. In conclusion, applying the S. pactum Act12 agent reduced TYLCD severity and promoted plant growth in tomato. Whether the changes of resistance-related gene expression and rhizosphere microbial community composition contribute to TYLCV resistance needs to be further investigated.

Keywords

Tomato yellow leaf curl virus disease Systemic acquired resistance Induced systemic resistance RNA interference Rhizosphere microbial community Modularization 

Notes

Acknowledgments

We thank Dr. Chaofeng Lin (TEC, Qingdao, China) for improving the English and Lu Wang (Plant Science Facility of the Institute of Botany, Chinese Academy of Sciences) for technical assistance with MS/MS analysis.

Funding information

This work was supported by the National Natural Science Fund Youth Project (31600407), the Fundamental Research Fund for the Central Universities (Z109021616), and the National Key Technology R&D Program (2012BAD14B11).

Compliance with ethical standards

This article does not contain any studies with human or animal subjects.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

374_2019_1339_MOESM1_ESM.docx (65 kb)
ESM 1 (DOCX 64 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Natural Resources and EnvironmentNorthwest A&F UniversityYanglingChina
  2. 2.College of AgricultureGuizhou UniversityGuiyangChina

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