Organic carbon quality, composition of main microbial groups, enzyme activities, and temperature sensitivity of soil respiration of an acid paddy soil treated with biochar
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The role of soil organic C (SOC) quality affecting microbial community composition and function under biochar application is poorly understood. We investigated the relationship between the pool size and chemical composition of SOC; composition of main microbial groups; enzyme activities involved in C, N, and P cycling; and soil respiration in a rice paddy amended with biochar for 20 months in a laboratory experiment at 15, 25, and 35 °C. Soil labile and recalcitrant organic C pools were determined by a two-step sulfuric acid (H2SO4) hydrolysis method. The chemical composition of SOC was determined with 13C-nuclear magnetic resonance spectroscopy. The biochar amendment at 20 and 40 t ha−1 significantly decreased the soil labile C pool I (extracted by 5 N H2SO4), alkyl, and carbonyl C contents and increased the recalcitrant C pool (acid-resistant) and aromatic C contents and the aromatic C to O-alkyl C ratio. The phospholipid-fatty acid concentrations and soil enzyme activities were unchanged by biochar application at 10 and 20 t ha−1, but both were increased at 40 t ha−1. Biochar increased the ratio of gram-positive (G+) to gram-negative (G−) bacteria and decreased that of fungi to bacteria. The recalcitrant C pool and aromatic C contents were positively correlated to the G+ bacteria abundance and were important factors in shaping composition of the main microbial groups and improving enzyme activities. Biochar application at 40 t ha−1 lowered soil respiration rates at 15 and 25 °C by decreasing labile C pool and increasing C recalcitrancy while increased temperature sensitivities of soil respiration at 25/15 °C and 35/25 °C by stimulating microbial abundance and enzyme activities. Together, our results suggest that biochar soil amendment shifted microbial community composition and function through influencing the composition of SOC.
KeywordsBiochar 13C NMR Phospholipid-fatty acids Soil microbial respiration Soil organic carbon Temperature sensitivity
We thank Dr. Paolo Nannipieri, the Editor-in-Chief, and the anonymous reviewers for their very valuable comments in improving both the language and scientific quality of the manuscript.
This work was funded by the National Natural Science Foundation of China under grant numbers 41401318, 41371298, and 31470626, and the Natural Science Foundation of Zhejiang Province under grant number LGF18C160001.
- Bremner JM (1996) Nitrogen total. In: Sparks DL (ed) Methods of soil analysis, part 3 chemical methods, the soil science Society of American Book Series no 5. Soil Science Society of America, Inc., Madison, WI, pp 1085–1122Google Scholar
- Chen J, Liu X, Li L, Zheng J, Qu J, Zheng J, Zhang X, Pan G (2015) Consistent increase in abundance and diversity but variable change in community composition of bacteria in topsoil of rice paddy under short term biochar treatment across three sites from South China. Appl Soil Ecol 91:68–79CrossRefGoogle Scholar
- Chen J, Li S, Liang C, Xu Q, Li Y, Qin H, Fuhrmann JJ (2017) Response of microbial community structure and function to short-term biochar amendment in an intensively managed bamboo (Phyllostachys praecox) plantation soil: effect of particle size and addition rate. Sci Total Environ 574:24–33CrossRefGoogle Scholar
- Chen J, Sun X, Zheng J, Zhang X, Liu X, Bian R, Li L, Cheng K, Zheng J, Pan G (2018) Biochar amendment changes temperature sensitivity of soil respiration and composition of microbial communities 3 years after incorporation in an organic carbon-poor dry cropland soil. Biol Fertil Soils 54:175–188CrossRefGoogle Scholar
- Cheng L, Zhang N, Yuan M, Xiao J, Qin Y, Deng Y, Tu Q, Xue K, Van Nostrand JD, Wu L, He Z, Zhou X, Leigh MB, Konstantinidis KT, Schuur EAG, Luo Y, Tiedje JM, Zhou J (2017) Warming enhances old organic carbon decomposition through altering functional microbial communities. ISME J 11:1825–1835CrossRefGoogle Scholar
- Conant RT, Ryan MG, Ågren GI, Birge HE, Davidson EA, Eliasson PE, Evans SE, Frey SD, Giardina CP, Hopkins FM, Hyvönen R, Kirschbaum MUF, Lavallee JM, Leifeld J, Parton WJ, Megan Steinweg J, Wallenstein MD, Martin Wetterstedt JÅ, Bradford MA (2011) Temperature and soil organic matter decomposition rates—synthesis of current knowledge and a way forward. Glob Chang Biol 17:3392–3404CrossRefGoogle Scholar
- Gong ZT, Zhang GL, Chen ZC (2007) Pedogenesis and soil taxonomy. Science Press, Beijing, ChinaGoogle Scholar
- Karhu K, Auffret MD, Dungait JAJ, Hopkins DW, Prosser JI, Singh BK, Subke J-A, Wookey PA, Agren GI, Sebastia M-T, Gouriveau F, Bergkvist G, Meir P, Nottingham AT, Salinas N, Hartley IP (2014) Temperature sensitivity of soil respiration rates enhanced by microbial community response. Nature 513:81–84CrossRefGoogle Scholar
- Keiblinger KM, Hall EK, Wanek W, Szukics U, Hämmerle I, Ellersdorfer G, Böck S, Strauss J, Sterflinger K, Richter A, Zechmeister-Boltenstern S (2010) The effect of resource quantity and resource stoichiometry on microbial carbon-use-efficiency. FEMS Microbiol Ecol 73:430–440Google Scholar
- Li Y, Li Y, Chang SX, Yang Y, Fu S, Jiang P, Luo Y, Yang M, Chen Z, Hu S, Zhao M, Liang X, Xu Q, Zhou G, Zhou J (2018) Biochar reduces soil heterotrophic respiration in a subtropical plantation through increasing soil organic carbon recalcitrancy and decreasing carbon-degrading microbial activity. Soil Biol Biochem 122:173–185CrossRefGoogle Scholar
- Nelson DW, Sommers LE (1982) Total carbon, organic carbon and organic matter. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, part 2 chemical and microbiological properties. American Society of Agronomy, Inc., Soil Science Society of America, Inc., Madison, WI, pp 539–579Google Scholar
- Zak DR, Pregitzer KS, Curtis PS, Holmes WE (2000) Atmospheric CO2 and the composition and function of soil microbial communities. Ecol Appl 10:47–59Google Scholar
- Zheng J, Chen J, Pan G, Liu X, Zhang X, Li L, Bian R, Cheng K, Zheng J (2016) Biochar decreased microbial metabolic quotient and shifted community composition four years after a single incorporation in a slightly acid rice paddy from Southwest China. Sci Total Environ 571:206–217CrossRefGoogle Scholar
- Zhou G, Zhou X, Zhang T, Du Z, He Y, Wang X, Shao J, Cao Y, Xue S, Wang H, Xu C (2017) Biochar increased soil respiration in temperate forests but had no effects in subtropical forests. Forest Ecol Manag 405:339–349Google Scholar