Effect of nitrogen fertilization on the fate of rice residue-C in paddy soil depending on depth: 13C amino sugar analysis

  • Xiangbi Chen
  • Yinhang Xia
  • Yajun Hu
  • Anna Gunina
  • Tida Ge
  • Zhenhua Zhang
  • Jinshui Wu
  • Yirong Su
Original Paper
  • 37 Downloads

Abstract

A 100-day incubation experiment was conducted to (i) trace the fate of rice residue-derived 13C in the amino sugar (AS) pool in 0–1-cm (oxic) and 1–5-cm (anoxic) layers of paddy soil and (ii) evaluate the effects of inorganic N ((NH4)2SO4) fertilization on the formation of AS at early and late incubation times (5 and 100 days, respectively). The accumulation of rice residue-derived AS occurred at 5 and 100 days in both soil layers as a result of AS stabilization. Inorganic N addition increased the contents of rice residue-derived muramic acid, glucosamine, and galactosamine in the 0–1-cm soil layer for both incubation times by average on 14.7–20.8%, 23.7–31.8%, and 11.6–23.3%, respectively. In contrast, no effects of N fertilization on AS content in the 1–5-cm soil layer were found. The amount of rice residue-derived AS was higher in the 1–5-cm than in the 0–1-cm soil layer at early incubation time, probably due to the higher contents of ammonium here compared to the upmost oxic layer where nitrate was the dominated N form. Thus, the preferential uptake of ammonium but not nitrate by microorganisms led to the higher formation of rice residue-derived AS in the anoxic soil layer. The ratio of fungal to bacterial residues (fungal glucosamine/muramic acid) ranged between 1.0 and 1.7 for rice residue-derived AS and was 12.5–14.6 for total AS, indicating that fungi and bacteria have similar contributions to the decomposition of fresh rice residue whereas native soil organic matter (SOM) is a fungi-predominated process. This study emphasized that coupling of C and N cycles in paddy soils is different in oxic and anoxic layers, resulting in variation of plant residue decomposition and formation of SOM.

Keywords

Microbial biomarkers Amino sugar 13C compound-specific isotope labeling Inorganic N fertilization Soil depth Paddy soil 

Supplementary material

374_2018_1278_MOESM1_ESM.docx (1.3 mb)
ESM 1 (DOCX 1.28 mb)

References

  1. Amelung W, Zhang X, Zech W, Flach KW (1999) Amino sugars in native grassland soils along a climosequence in North America. Soil Sci Soc Am J 63:86–92CrossRefGoogle Scholar
  2. Amelung W, Miltner A, Zhang X, Zech W (2001) Fate of microbial residues during litter decomposition as affected by minerals. Soil Sci 166:598–606CrossRefGoogle Scholar
  3. Atere CT, Ge T, Zhu Z, Tong C, Jones DL, Shibistova O, Guggenberger G, Wu J (2017) Rice rhizodeposition and carbon stabilisation in paddy soil are regulated via drying-rewetting cycles and nitrogen fertilization. Biol Fertil Soils 53:407–417CrossRefGoogle Scholar
  4. Bai R, Xi D, He J, Hu H, Fang Y, Zhang L (2015) Activity, abundance and community structure of anammox bacteria along depth profiles in three different paddy soils. Soil Biol Biochem 91:212–221CrossRefGoogle Scholar
  5. Balasooriya WK, Huygens D, Rajapaksha RMCP, Boeckx P (2016) Effect of rice variety and fertilizer type on the active microbial community structure in tropical paddy fields in Sri Lanka. Geoderma 265:87–95CrossRefGoogle Scholar
  6. Burger M, Jackson LE (2003) Microbial immobilization of ammonium and nitrate in relation to ammonification and nitrification rates in organic and conventional cropping systems. Soil Biol Biochem 35:29–36CrossRefGoogle Scholar
  7. Chantigny MH, Angers DA, Prévost D, Vézina LP, Chalifour FP (1997) Soil aggregation and fungal and bacterial biomass under annual and perennial cropping systems. Soil Sci Soc Am J 61:262–267CrossRefGoogle Scholar
  8. Chen Z, Luo X, Hu R, Wu M, Wu J, Wei W (2010) Impact of long–term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil. Microb Ecol 60:850–861CrossRefPubMedGoogle Scholar
  9. Cheng Y, Wang J, Mary B, Zhang J, Cai Z, Chang SX (2013) Soil pH has contrasting effects on gross and net nitrogen mineralizations in adjacent forest and grassland soils in central Alberta, Canada. Soil Biol Biochem 57:848–857CrossRefGoogle Scholar
  10. Ding X, Zhang X, He H, Xie H (2010) Dynamics of soil amino sugar pools during decomposition processes of corn residues as affected by inorganic N addition. J Soils Sediments 10:758–766CrossRefGoogle Scholar
  11. Ding X, Han X, Zhang X (2013) Long-term impacts of manure, straw, and fertilizer on amino sugars in a silty clay loam soil under temperate conditions. Biol Fertil Soils 49:949–954CrossRefGoogle Scholar
  12. Engelking B, Flessa H, Joergensen RG (2007) Shifts in amino sugar and ergosterol contents after addition of sucrose and cellulose to soil. Soil Biol Biochem 39:2111–2118CrossRefGoogle Scholar
  13. Frenzel P, Rothfuss F, Conrad R (1992) Oxygen profiles and methane turnover in a flooded rice microcosm. Biol Fertil Soils 14:84–89CrossRefGoogle Scholar
  14. Ge T, Li B, Zhu Z, Hu Y, Yuan H, Dorodnikov M, Jones DL, Wu J, Kuzyakov Y (2017) Rice rhizodeposition and its utilization by microbial groups depends on N fertilization. Biol Fertil Soils 53:37–48CrossRefGoogle Scholar
  15. Glaser B, Turrión MB, Alef K (2004) Amino sugars and muramic acid-biomarkers for soil microbial community structure analysis. Soil Biol Biochem 36:399–407CrossRefGoogle Scholar
  16. Glaser B, Millar N, Blum H (2006) Sequestration and turnover of bacterial- and fungal-derived carbon in a temperate grassland soil under long-term elevated atmospheric pCO2. Glob Chang Biol 12:1521–1531CrossRefGoogle Scholar
  17. Gunina A, Dippold M, Glaser B, Kuzyakov Y (2017) Turnover of microbial groups and cell components in soil: 13C analysis of cellular biomarkers. Biogeosciences 14:271–283CrossRefGoogle Scholar
  18. Güsewell S, Gessner MO (2009) N: P ratios influence litter decomposition and colonization by fungi and bacteria in microcosms. Funct Ecol 23:211–219CrossRefGoogle Scholar
  19. Hoque MM, Inubushi K, Miura S, Kobayashi K, Kim H-Y, Okada M, Yabashi S (2002) Nitrogen dynamics in paddy field as influenced by free–air CO2 enrichment (FACE) at three levels of nitrogen fertilization. Nutr Cycl Agroecosyst 63:301–308CrossRefGoogle Scholar
  20. Inubushi K, Cheng W, Aonuma S, Hoque MM, Kobayashi K, Miura S, Kim HY, Okada M (2003) Effects of free-air CO2 enrichment (FACE) on CH4 emission from a rice paddy field. Glob Chang Biol 9:1458–1464CrossRefGoogle Scholar
  21. Janssen M, Lennartz B (2007) Horizontal and vertical water and solute fluxes in paddy rice fields. Soil Till Res 94:133–141CrossRefGoogle Scholar
  22. Jia J, Feng X, He J, He H, Lin L, Liu Z (2017) Comparing microbial carbon sequestration and priming in the subsoil versus topsoil of a Qinghai-Tibetan alpine grassland. Soil Biol Biochem 104:141–151Google Scholar
  23. Kögel-Knabner I, Amelung W, Cao Z, Fiedler S, Frenzel P, Jahn R, Kalbitz K, Kölbl A, Schloter M (2010) Biogeochemistry of paddy soils. Geoderma 157:1–14CrossRefGoogle Scholar
  24. Kuzyakov Y, Friedel J, Stahr K (2000) Review of mechanisms and quantification of priming effects. Soil Biol Biochem 32:1485–1498CrossRefGoogle Scholar
  25. Lal R (2004) Soil carbon sequestration impacts on global climate change and food security. Science 304:1623–1627CrossRefPubMedGoogle Scholar
  26. Li Z, Liu M, Wu X, Han F, Zhang T (2010) Effects of long-term chemical fertilization and organic amendments on dynamics of soil organic C and total N in paddy soil derived from barren land in subtropical China. Soil Till Res 106:268–274CrossRefGoogle Scholar
  27. Li X, Sun J, Wang H, Li X, Wang J, Zhang H (2017) Changes in the soil microbial phospholipid fatty acid profile with depth in three soil types of paddy fields in China. Geoderma 290:69–74CrossRefGoogle Scholar
  28. Liesack W, Schnell S, Revsbech NP (2000) Microbiology of flooded rice paddies. FEMS Microb Rev 24:625–645CrossRefGoogle Scholar
  29. Lüdemann H, Arth I, Liesack W (2000) Spatial changes in the bacterial community structure along a vertical oxygen gradient in flooded paddy soil cores. Appl Environ Microb 66:754–762CrossRefGoogle Scholar
  30. Myrold DD, Posavatz NR (2007) Potential importance of bacteria and fungi in nitrate assimilation in soil. Soil Biol Biochem 39:1737–1743CrossRefGoogle Scholar
  31. Nakamura A, Tun CC, Asakawa S, Kimura M (2003) Microbial community responsible for the decomposition of rice straw in a paddy field: estimation by phospholipid fatty acid analysis. Biol Fertil Soils 38:288–295CrossRefGoogle Scholar
  32. Noll M, Matthies D, Frenzel P, Derakshani M, Liesack W (2005) Succession of bacterial community structure and diversity in a paddy soil oxygen gradient. Environ Microb 7:382–395CrossRefGoogle Scholar
  33. Pan G, Li L, Wu L, Zhang X (2004) Storage and sequestration potential of topsoil organic carbon in China’s paddy soils. Glob Chang Biol 10:79–92CrossRefGoogle Scholar
  34. Pan G, Zhou P, Li Z, Smith P, Li L, Qiu D, Zhang X, Xu X, Shen S, Chen X (2009) Combined inorganic/organic fertilization enhances N efficiency and increases rice productivity through organic carbon accumulation in a rice paddy from the Tai Lake region, China. Agric Ecosyst Environ 131:274–280CrossRefGoogle Scholar
  35. Recous S, Mary B, Faurie G (1990) Microbial immobilization of ammonium and nitrate in cultivated soils. Soil Biol Biochem 22:913–922CrossRefGoogle Scholar
  36. Rousk K, Michelsen A, Rousk J (2016) Microbial control of soil organic matter mineralization responses to labile carbon in subarctic climate change treatments. Glob Chang Biol 22:4150–4161CrossRefPubMedGoogle Scholar
  37. Schmidt I, Sliekers O, Schmid M, Cirpus I, Strous M, Dock E, Kuenen JG, Jetten MSM (2002) Aerobic and anaerobic ammonia oxidizing bacteria-competitors or natural partners? FEMS Microbiol Ecol 39:175–181PubMedGoogle Scholar
  38. Strickland MS, Rousk J (2010) Considering fungal: bacterial dominance in soils-methods, controls, and ecosystem implications. Soil Biol Biochem 42:1385–1395CrossRefGoogle Scholar
  39. Treseder KK (2008) Nitrogen additions and microbial biomass: a meta-analysis of ecosystem studies. Ecol Lett 11:1111–1120CrossRefPubMedGoogle Scholar
  40. Wang B, Zhao J, Guo Z, Ma J, Xu H, Jia Z (2015) Differential contributions of ammonia oxidizers and nitrite oxidizers to nitrification in four paddy soils. ISME J 9:1062–1075CrossRefPubMedGoogle Scholar
  41. Wu X, Ge T, Yuan H, Li B, Zhu H, Zhou P, Sui F, O’Donnell AG, Wu J (2014) Changes in bacterial CO2 fixation with depth in agricultural soils. Appl Microb Biotechnol 98:2309–2319CrossRefGoogle Scholar
  42. Zhang X, Amelung W (1996) Gas chromatographic determination of muramic acid, glucosamine, mannosamine, and galactosamine in soils. Soil Biol Biochem 28:1201–1206CrossRefGoogle Scholar
  43. Zhang W, Xu M, Wang X, Huang Q, Nie J, Li Z, Li S, Hwang SW, Lee KB (2012) Effects of organic amendments on soil carbon sequestration in paddy fields of subtropical China. J Soils Sediments 12:457–470CrossRefGoogle Scholar
  44. Zhong WH, Cai ZC (2007) Long-term effects of inorganic fertilizers on microbial biomass and community functional diversity in a paddy soil derived from quaternary red clay. Appl Soil Ecol 36:84–91CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xiangbi Chen
    • 1
    • 2
  • Yinhang Xia
    • 1
    • 3
  • Yajun Hu
    • 1
    • 2
  • Anna Gunina
    • 4
  • Tida Ge
    • 1
  • Zhenhua Zhang
    • 2
  • Jinshui Wu
    • 1
  • Yirong Su
    • 1
  1. 1.Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical AgricultureThe Chinese Academy of SciencesChangshaPeople’s Republic of China
  2. 2.Southern Regional Collaborative Innovation Center for Grain and Oil Crops in ChinaHunan Agricultural UniversityChangshaPeople’s Republic of China
  3. 3.University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  4. 4.Department of Environmental ChemistryUniversity of KasselKasselGermany

Personalised recommendations