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Participation of Na+/K+-ATPase and aquaporins in the uptake of water during moult processes in the shrimp Palaemon argentinus (Nobili, 1901)

  • Kamila Foguesatto
  • Cláudio Luis Quaresma Bastos
  • Robert Tew Boyle
  • Luiz Eduardo Maya Nery
  • Marta Marques SouzaEmail author
Original Paper
  • 37 Downloads

Abstract

Due to the presence of the exoskeleton, the moult cycle is a required event in the life of crustaceans. In order for the exoskeleton to be replaced, it is necessary for these animals to uptake water from the environment for their body tissues during the late pre-moult, ecdysis and in the early post-moult for the expansion of the new cuticle. The mechanisms and organs used to uptake water in these events are not yet completely clear. In this study, we investigated the participation of aquaporins and Na+/K+-ATPase in cells of two potential organs responsible for the uptake of water (gills and gut) at three different stages of the moult cycle in freshwater shrimp Palaemon argentinus. We showed the participation of these two proteins with different functional patterns in gills and intestinal cells as water uptake pathways for moult and early post-moult. Our results indicate that Na+/K+-ATPase promotes the necessary osmotic gradient in the gills for water uptake through the gut cells during the pre-moult. This process, in turn, remains active during the post-moult stage with the addition of water influx through the gill cells.

Keywords

Crustacean Moult Cell culture Gill Gut Transporter proteins 

Notes

Acknowledgements

The authors would like to thank Dr. Mateus Tavares Kütter for his support during the animal collection, and Msc Isadora P. M. Medeiros for her support during the ion measurements. Kamila Foguesatto has a fellowship from Coordination of Improvement of Higher Level Personnel (CAPES) Brazil. Marta M. Souza and Luiz E. M. Nery are researches fellows from the Brazilian CNPq (Proc. # 311806/2017-1 and 310830/2017-6, respectively).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. 1.Programa de Pós-Graduação em Ciências Fisiológicas, Instituto de Ciências BiológicasUniversidade Federal do Rio Grande–FURGRio GrandeBrazil
  2. 2.Instituto de Ciências BiológicasUniversidade Federal do Rio Grande–FURGRio GrandeBrazil
  3. 3.Instituto de Ciências BiológicasUniversidade Federal do Rio Grande–FURGRio GrandeBrazil

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