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Effect of optical fiber diameter and laser emission mode (cw vs pulse) on tissue damage profile using 1.94 µm Tm:fiber lasers in a porcine kidney model

  • Benedikt BeckerEmail author
  • Dmitry Enikeev
  • Petr Glybochko
  • Leonid Rapoport
  • Mark Taratkin
  • Andreas J. Gross
  • Viktoriya Vinnichenko
  • Thomas R. W. Herrmann
  • Christopher Netsch
Original Article
  • 13 Downloads

Abstract

Purpose

To evaluate the ablation capacity using two Thulium fiber lasers (TFL) in a porcine kidney model.

Methods

All tissue samples were mounted on a motorized stage for a precise speed of cutting. A continuous wave (cw) TFL and a super pulsed (SP) TFL were used at power settings of 60 and 120 W with 200 and 600 µm laser fibers. After lactate dehydrogenase staining, histological evaluation was performed to measure the vaporization volume (VV), ablation depth (AD), thermo-mechanical damage zones (TMZ), coagulation zones (CZ) and the carbonization grade (CG).

Results

At 120 W, no significant differences were seen between 200 and 600 µm fibers utilizing the cw TFL regarding VV (24.6 vs. 28.2 mm3/s), AD (5.6 vs. 5.7 mm), TMZ (0 vs. 0 mm2) and CZ (18.1 vs. 12.3 mm2). Using the SP TFL, no significant differences between both fiber diameters with regard to VV (4 vs. 6.2 mm3/s), AD (2.7 vs. 3.4 mm), TMZ (1 vs. 2.6 mm2) and CZ (3.1 vs. 2.2 mm2) at 120 W were found, respectively. However, the VV of the cw TFL at 60 W was significantly less compared to 120 W using 200 and 600 µm fibers, respectively, whereas the SP TFL did not show significant differences between 60 and 120 W with regard to VV. SP TFL showed a consistently lower CG compared to cw TFL.

Conclusions

This experiment suggests that there is no significant difference using 200 or 600 µm laser fibers in cw or SP TFLs. However, the cw TFL produces a coagulation zone three to five times larger than the SP TFL regardless of the fiber diameter.

Keywords

In-vitro Thulium fiber laser Porcine kidney Laser 

Notes

Author contributions

All authors whose names appear on the submission have contributed sufficiently to the scientific work and therefore share collective responsibility and accountability for the results. BB: project development, data collection, data analysis, manuscript writing/editing. DE: project development, data collection, data analysis, manuscript editing. PG: project development, manuscript editing. LR: project development, manuscript editing. MT: project development, data collection, data analysis, manuscript editing. AJG: project development, manuscript editing. VV: project development, data collection, data analysis. TRWH: project development, manuscript editing. CN: project development, data collection, data analysis, manuscript editing.

Funding

No funding was obtained.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Informed consent was not necessary in this study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Benedikt Becker
    • 1
    Email author
  • Dmitry Enikeev
    • 2
  • Petr Glybochko
    • 2
  • Leonid Rapoport
    • 2
  • Mark Taratkin
    • 2
  • Andreas J. Gross
    • 1
  • Viktoriya Vinnichenko
    • 3
  • Thomas R. W. Herrmann
    • 4
  • Christopher Netsch
    • 1
  1. 1.Department of UrologyAsklepios Hospital BarmbekHamburgGermany
  2. 2.Institute for Urology and Reproductive HealthSechenov UniversityMoscowRussia
  3. 3.Moscow Engineering Physics InstituteMoscowRussia
  4. 4.Department of UrologyKantonsspital FrauenfeldFrauenfeldSwitzerland

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