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Growth and Hormone Alterations in Response to Heat Stress in Perennial Ryegrass Accessions Differing in Heat Tolerance

  • Manli Li
  • Amber H. Jannasch
  • Yiwei JiangEmail author
Article

Abstract

Plant growth regulators can mediate plant response to stress. The objective of this study was to investigate hormone alterations associated with plant growth and heat tolerance in perennial ryegrass (Lolium perenne). The heat tolerant accession PI265351 and sensitive accession PI225825 were exposed to 35°C/30°C at 7-, 14-, and 21-days in a growth chamber. Heat stress decreased plant height (HT) and leaf water content (LWC) in both accessions, but reductions in HT and LWC delayed one week in PI265351. Chlorophyll fluorescence remained unchanged in PI265351 but decreased in PI225825 under heat stress, compared to their controls. Heat stress increased leaf trans-zeatin and abscisic acid (ABA) and decreased gibberellin (GA3) concentrations, occurring at 14- and 21-days for PI265351 and during the entire stress period for PI225825, respectively, compared to the unstressed controls. Both accessions showed similar trends in kinetin with an increased concentration at 14- and 21-days. An elevated level of indole-3-acetic acid (IAA) was observed in PI265351 at 21 days of heat stress, while indole-3-butyric acid (IBA) increased at 14- and 21-days for PI265351 and only at 14 days for PI225825. Heat stress increased salicylic acid (SA) concentration at 14 days for PI265351 but caused reductions in SA during the stress period for PI225825. The results indicated that the maintenance of IAA, IBA, and SA, delayed increases in ABA, and delayed reductions of GA3 could contribute to heat tolerance of perennial ryegrass.

Keywords

Growth Heat stress Hormone Lolium perenne 

Notes

Acknowledgements

This research is supported by the Midwest Regional Turfgrass Foundation of Purdue University. Dr. Manli Li is grateful to the China Scholarship Council (Award No.[2016] 3035) for supporting her visit at Purdue University.

Author Contributions

ML conducted the experiments, performed the statistical analysis of data, and prepared the manuscript. AJ assisted in hormone analysis and manuscript preparation. YJ supervised the overall work and contributed to data interpretation and paper writing.

Compliance with Ethical Standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

Supplementary material

344_2019_10043_MOESM1_ESM.docx (810 kb)
Supplementary file1 (DOCX 810 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Grassland Science and TechnologyChina Agricultural UniversityBeijingChina
  2. 2.Bindley Bioscience CenterPurdue UniversityWest LafayetteUSA
  3. 3.Department of AgronomyPurdue UniversityWest LafayetteUSA

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