Silencing of the MADS-Box Gene SlMADS83 Enhances Adventitious Root Formation in Tomato Plants
- 16 Downloads
Adventitious roots (ARs) are important for the growth of plants and the improvement in their stress resistance and survival capacity. Although many genes have been confirmed to be involved in adventitious root (AR) formation in Arabidopsis and tomato plants, MADS-box genes have rarely been mentioned. Here, we isolated a MADS-box gene named SlMADS83, which may negatively regulate AR formation in tomato plants, as the number of the ARs formed in the transgenic lines in which the SlMADS83 gene was silenced by RNA interference (RNAi) was increased. The above phenotype was further confirmed by the analysis of the macroscopic, anatomical, and molecular features and related statistical data. Previous Studies have proven that auxin can stimulate early AR primordium initiation. Interestingly, in the RNAi transgenic lines, the concentration of auxin in the hypocotyl base was increased, resulting in early induction of AR primordia initiation, promoting the formation of ARs. Briefly, SlMADS83 may play an important role in AR formation.
KeywordsAdventitious roots Tomato SlMADS83 Auxin biosynthesis
This work was supported by National Natural Science Foundation of China (No. 31572129) and the Natural Science Foundation of Chongqing of China (No. cstc2015jcyjA80026).
Z.H. and G.C. designed the research; A.L., Y.W., and H.L. performed the research; A.L. wrote the paper; Z.H. modified the paper. All authors have read and approved the final version of the manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Ahkami AH, Lischewski S, Haensch KT, Porfirova S, Hofmann J, Rolletschek H, Melzer M, Franken P, Hause B, Druege U, Hajirezaei MR (2009) Molecular physiology of adventitious root formation in Petunia hybrida cuttings: involvement of wound response and primary metabolism. New Phytol 181:613–625PubMedCrossRefGoogle Scholar
- Correa LD, Troleis J, Mastroberti AA, Mariath JEA, Fett AG (2012) Distinct modes of adventitious rooting in Arabidopsis thaliana. Plant Biol 14:100–109Google Scholar
- Gutierrez L, Bussell JD, Pacurar DI, Schwambach J, Pacurar M, Bellini C (2009) Phenotypic plasticity of adventitious rooting in Arabidopsis is controlled by complex regulation of AUXIN RESPONSE FACTOR transcripts and MicroRNA abundance. Plant Cell 21:3119–3132PubMedPubMedCentralCrossRefGoogle Scholar
- He W, Brumos J, Li H, Ji Y, Ke M, Gong X, Zeng Q, Li W, Zhang X, An F, Wen X, Li P, Chu J, Sun X, Yan C, Yan N, Xie DY, Raikhel N, Yang Z, Stepanova AN, Alonso JM, Guo H (2011) A small-molecule screen identifies L-kynurenine as a competitive inhibitor of TAA1/TAR activity in ethylene-directed auxin biosynthesis and root growth in Arabidopsis. Plant Cell 23:3944–3960PubMedPubMedCentralCrossRefGoogle Scholar
- Koo SC, Bracko O, Park MS, Schwab R, Chun HJ, Park KM, Seo JS, Grbic V, Balasubramanian S, Schmid M, Godard F, Yun DJ, Lee SY, Cho MJ, Weigel D, Kim MC (2010) Control of lateral organ development and flowering time by the Arabidopsis thaliana MADS-box gene AGAMOUS-LIKE6. Plant J 62:807–816PubMedCrossRefGoogle Scholar
- Sun CH, Yu JQ, Wen LZ, Guo YH, Sun X, Hao YJ, Hu DG, Zheng CS (2017) Chrysanthemum MADS-box transcription factor CmANR1 modulates lateral root development via homo-/heterodimerization to influence auxin accumulation in Arabidopsis. Plant Sci. https://doi.org/10.1016/j.plantsci.2017.09.017 CrossRefPubMedGoogle Scholar
- Sun CH, Yu JQ, Duan X, Wang JH, Zhang QY, Gu KD, Hu DG, Zheng CS (2018) The MADS transcription factor CmANR1 positively modulates root system development by directly regulating CmPIN2 in chrysanthemum. Hortic Res 5:52. https://doi.org/10.1038/s41438-018-0061-y CrossRefPubMedPubMedCentralGoogle Scholar