Genome-Wide Identification of Malectin/Malectin-Like Domain Containing Protein Family Genes in Rice and Their Expression Regulation Under Various Hormones, Abiotic Stresses, and Heavy Metal Treatments
Malectin/malectin-like domain containing proteins are a novel identified protein subfamily of lectins that regulates various functional bioprocesses in plants. However, little is known about the phylogenetic relationships and functions of this gene family in rice. Here, in this study, a total of 84 malectin/malectin-like domain containing proteins, including 67 malectin/malectin-like receptor-like kinases (MRLKs) and 17 malectin/malectin-like receptor-like proteins (MRLPs), were identified in rice. These MRLK and MRLP proteins can be classified into four subfamilies based on the malectin/malectin-like domain present in their extracellular regions. The OsMRLK and OsMRLP gene family members are localized on all the chromosomes in rice genome with the members within the same subfamily sharing similar exon–intron distribution and motif composition. The multiple members and diverse gene and protein structures demonstrate that the malectin/malectin-like domain protein family underscores a functional diversity and divergence in rice. In addition, the expression levels of the OsMRLK gene family members are greatly varied between the tissues and organs at different developmental stages of rice. Furthermore, the majority of the tested OsMRLKs showed significant upregulation in transcripts under a number of abiotic stresses, hormonal applications, and heavy metals, suggesting the vital roles of the OsMRLK genes in rice development and multi-stress tolerance. The results obtained here provide a well fundament for further clarification of the roles of the malectin/malectin-like domain containing proteins in plants.
KeywordsMalectin/malectin-like domain containing proteins Phylogenetic relationship Transcriptional expression Multivariate stress response Rice (Oryza sativa)
This work was supported by the National Natural Science Foundation of China (Grant Nos. 31770204 and 31270299).
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Feng W, Kita D, Peaucelle A, Cartwright HN, Doan V, Duan Q, Liu M-C, Maman J, Steinhorst L, Schmitz-Thom I (2018) The FERONIA receptor kinase maintains cell-wall integrity during salt stress through Ca2+ signaling. Curr Biol 28(666–675):e665Google Scholar
- Formentin E, Sudiro C, Perin G, Riccadonna S, Barizza E, Baldoni E, Lavezzo E, Stevanato P, Sacchi GA, Fontana P (2018) Transcriptome and cell physiological analyses in different rice cultivars provide new insights into adaptive and salinity stress responses. Front Plant Sci 9:204CrossRefGoogle Scholar
- Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Calif Agric Exp Stn Circ 347:357–359Google Scholar
- Kaya H, Nakajima R, Iwano M, Kanaoka MM, Kimura S, Takeda S, Kawarazaki T, Senzaki E, Hamamura Y, Higashiyama T (2014) Ca2+-activated reactive oxygen species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth. Plant Cell 113:120642Google Scholar
- Schallus T, Jaeckh C, Fehér K, Palma AS, Liu Y, Simpson JC, Mackeen M, Stier G, Gibson TJ, Feizi T (2008) Malectin: a novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation. Mol Biol Cell 19:3404–3414CrossRefGoogle Scholar
- Yeh Y-H, Panzeri D, Kadota Y, Huang Y-C, Huang P-Y, Tao C-N, Roux M, Chien H-C, Chin T-C, Chu P-W (2016) The Arabidopsis malectin-like/LRR-RLK IOS1 is critical for BAK1-dependent and BAK1-independent pattern-triggered immunity. Plant Cell 00313:02016Google Scholar