Chloride deicing salt stress usually coincides with the event of freeze–thaw, and the short-term adaptation of Dongmu-70 Secale cereale L. seedlings to these stresses was investigated in this paper. The chloride deicing salt and the freeze–thaw (FT) simulation experiments were carried out in the lab and alternation refrigerator. The changes of soluble sugar, soluble protein, relative conductivity (RC), malondialdehyde (MDA), and catalase (CAT) activity in seedlings were studied under freeze–thaw stress (10, 5, 0, − 5, 0, 5, and 10 °C) and 0, 200, 400, and 600 mmol L−1 of chloride deicing salt stress (CK, D1, D2, and D3). The results indicated that the content of physiological index in different treatment groups rose first and then decreased within a freeze–thaw cycle. During the recovery phase (T8: 24 h after freeze–thaw stress and T9: 6 days after freeze–thaw stress), there was significant difference either in MDA and CAT activity between D2 × FT and D2 or in RC, MDA, soluble sugar, and CAT activity between D3 × FT and D3. The seedlings showed different adaptability under different intensities of combined stress, and the sequence of the changes in physiological index can be patterned as D × FT > FT > D > CK. Freeze–thaw and chloride deicing salt complex stress exhibited a synergistic effect on the plant, which indicates that the snow-melting operation would be more harmful in spring and autumn to plants than in winter.
Secale cereale L. Chloride deicing salt Freeze–thaw Physiological response
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Financial support from the National Natural Science Foundation of China (Grant No. 31772669) to Professor Bao is gratefully acknowledged.
This work was sponsored by the National Natural Science Foundation of China (Grant No. 31772669).
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Conflict of interest
The authors declare that they have no conflict of interest.
Bothe A, Westermeier P, Wosnitza A, Willner E, Schum A, Dehmer KJ, Hartmann S (2018) Drought tolerance in perennial ryegrass (Lolium perenne L.) as assessed by two contrasting phenotyping systems. J Agron Crop Sci 204:375–389. https://doi.org/10.1111/jac.12269CrossRefGoogle Scholar
Chen J, Wang X (2006) Plant physiology experiment, 2nd edn. South China University of Technology, Guangzhou, pp 64–66 (in Chinese)Google Scholar
Chen J, Tao L, Zhu W (2004) Biochemical experiment, 3rd edn. Science, Beijing, pp 13–14 (in Chinese)Google Scholar
Chen S, Chen T, Yao X, Lv H, Li C (2018) Physicochemical properties of an asexual epichloe endophute-modified wild barley in the presence of salt stress. Pak J Bot 50:2105–2111Google Scholar
He A, Niu S, Zhao Q, Li Y, Gou J, Gao H, Suo S, Zhang J (2018) Induced salt tolerance of perennial ryegrass by a novel bacterium strain from the rhizosphere of a desert shrub haloxylon ammodendron. Int J Mol Sci. https://doi.org/10.3390/ijms19020469Google Scholar
Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil, vol 347, 2nd edn. California Agricultural Experiment Station, Circular, California, pp 1–32Google Scholar
Ibrahim W, Qiu C, Zhang C, Cao F, Shu Z, Wu F (2018a) Comparative physiological analysis in the tolerance to salinity and drought individual and combination in two cotton genotypes with contrasting salt tolerance. Physiol Plant 165:155–168. https://doi.org/10.1111/ppl.12791CrossRefGoogle Scholar
Ibrahim MEH, Zhu X, Zhou G, Ali AYA, Ahmad I, Farah GA (2018b) Nitrogen fertilizer alleviated negative impacts of NaCl on some physiological parameters of wheat. Pak J Bot 50:2097–2104Google Scholar