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Journal of Plant Growth Regulation

, Volume 38, Issue 4, pp 1206–1214 | Cite as

Study on the Physiological Mechanism of Early Flowering and Low Male Fertility of Limonium bicolor Mutant vrl15

  • Bingying Leng
  • Pengfei Zhao
  • Xinxiu Dong
  • Fang YuanEmail author
  • Baoshan WangEmail author
Article
  • 126 Downloads

Abstract

As biennial recretohalophytes, Limonium bicolor plants need 2 years to complete their life cycle. A growth habit mutant Vernalization Requirement Loss 15 (vrl15) was obtained by ion implantation. However, the biological characteristics of the mutant were unclear. In the current study, the related traits of vrl15 and some possible reasons for these traits were examined. Compared with wild type (WT), vrl15 can bolt and flower in approximately four months without vernalization. Moreover, vrl15 needed much less time to bolting and flowering than wild-type L. bicolor under different vernalization treatments. After 20 days’ vernalization, bolting vrl15 plants had 24 rosette leaves and bolting WT had 31 rosette leaves. Moreover, the pollen number per anther, the proportion of active pollen, the seed setting rate and the 1000 seed weight of vrl15 were all lower than those of WT. The soluble sugar content and soluble protein content in leaves of the vrl15 were much higher than those of WT sowed at the same time. In addition, the GA content in the leaves of bolting vrl15 was higher than that of the non-bolting WT sowed at the same time and non-bolting vrl15, whereas the contents of ABA and BR were much lower than that of the non-bolting WT. These results indicate that to some extent the increase of GA and decrease of ABA and BR content may be involved in the growth habit and male fertility alteration of mutant vrl15 of L. bicolor.

Keywords

Limonium bicolor Growth habit Vernalization Requirement Loss 15 Vernalization Endogenous hormone 

Notes

Acknowledgements

This work was supported by the NSFC (National Natural Science Research Foundation of China, Project Nos. 31570251; 31600200; 31770288), Shandong Province Key Research and Development Plan (2017CXGC0313), the Natural Science Research Foundation of Shandong Province (ZR2014CZ002; ZR2017MC003), and the Higher Educational Science and Technology Program of Shandong Province (J17KA136).

Author Contributions

BSW and BYL designed the experiment. BYL, PFZ and XXD performed the experiment. BYL, PFZ and FY finished the manuscript. All authors checked and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors have declared that no competing interests exist.

References

  1. Achard P, Harberd NP (2006) Integration of plant responses to environmentally activated phytohormonal signals. Science 311:91–94PubMedGoogle Scholar
  2. Alexander MP (1969) Differential staining of aborted and nonaborted pollen. Stain Technol 44:117–122PubMedGoogle Scholar
  3. Bastow R, Mylne JS, Lister C, Lippman Z, Martienssen RA, Dean C (2004) Vernalization requires epigenetic silencing of FLC by histone methylation. Nature 427:164–167PubMedGoogle Scholar
  4. Blazquez MR, Nilsson O, Sussman MR, Weigel D (1998) Gibberellins promote flowering of Arabidopsis by activating the leafy promoter. Plant Cell 10:791–800PubMedPubMedCentralGoogle Scholar
  5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254Google Scholar
  6. Campos KO, Kerbauy GB (2004) Thermoperiodic effect on flowering and endogenous hormonal status in Dendrobium (Orchidaceae). J Plant Physiol 161:1385–1387PubMedGoogle Scholar
  7. Chaari-Rkhis A, Maalej M, Messaoud SO, Drira N (2006) In vitro vegetative growth and flowering of olive tree in response to GA3 treatment. Afr J Biotechnol 5:2097–2302Google Scholar
  8. Chai Ym, Zhang Q, Tian L, Li CL, Xing Y, Qin L, Shen YY (2013) Brassinosteroid is involved in strawberry fruit ripening. Plant Growth Regul 69:63–69Google Scholar
  9. Dalla Guda C, Scordo E, Allera C, Farina E, Maloupa E (2000) Effects of low temperatures and gibberellic acid on flowering of Limonium gmelinii. Acta Hortic 541:193–199Google Scholar
  10. Darapuneni MK, Morgan GD, Ibrahim AMH, Duncan RW (2014) Effect of vernalization and photoperiod on flax flowering time. Euphytica 195:279–285Google Scholar
  11. Dogra S, Pandey RK, Bhat DJ (2012) Influence of gibberellic acid and plant geometry on growth, flowering and corm production in gladiolus (Gladiolus grandiflorus) under Jammu agroclimate. Int J Pharm Biol Sci 3:1083–1090Google Scholar
  12. Domagalska MA, Sarnowska E, Nagy F, Davis SJ (2010) Genetic analyses of interactions among gibberellin, abscisic acid, and brassinosteroids in the control of flowering time in Arabidopsis thaliana. PLoS ONE 5:e14012PubMedPubMedCentralGoogle Scholar
  13. Erwin JE, Warner RM (2000) Determination of photoperiodic response group and effect of supplemental irradiance on flowering of several bedding plant species. In: IV International ISHS Symposium on Artificial Lighting, vol 580, pp 95–99Google Scholar
  14. Galvão VC, Horrer D, Küttner F, Schmid M (2012) Spatial control of flowering by DELLA proteins in Arabidopsis thaliana. Development 139:4072–4082PubMedGoogle Scholar
  15. Garcialuis A, Fornes F, Guardiola JL (1995) Leaf carbohydrates and flower formation in Citrus. J Am Soc Hortic Sci 120:222–227Google Scholar
  16. Garner JM, Armitage AM (1996) Gibberellin applications influence the scheduling and flowering of Limonium X ‘Misty Blue’. Hortscience 31:247–248Google Scholar
  17. Hayama R, Yokoi S, Tamaki S, Yano M, Shimamoto K (2003) Adaptation of photoperiodic control pathways produces short-day flowering in rice. Nature 422:719–722PubMedGoogle Scholar
  18. Hu W, Hou X, Shi G (2004) Characteristics and mechanism of plant vernalization. Chin Bull Bot 36:283–289Google Scholar
  19. Kęsy J, Trzaskalska A, Galoch E, Kopcewicz J (2003) Inhibitory effect of brassinosteroids on the flowering of the short-day plant Pharbitis nil. Biol Plant 47:597–600Google Scholar
  20. King RWH, Hisamatsu T, Goldschmidt EE, Blundell C (2008) The nature of floral signals in Arabidopsis. I. Photosynthesis and a far-red photoresponse independently regulate flowering by increasing expression of FLOWERING LOCUS T (FT). J Exp Bot 59:3811–3820PubMedPubMedCentralGoogle Scholar
  21. Lejeune P, Bernier G, Kinet JM (1991) Sucrose levels in leaf exudate as a function of floral induction in the long day plant Sinapis alba. Plant Physiol Biochem 29:153–157Google Scholar
  22. Li XH (2003) Research on the introduction and application of wild flower Limonium bicolor. J Chin Landsc Archit 10:029Google Scholar
  23. Li ML, Zeng GW, Zhu ZJ (2002) Relationship of the levels of 5-methylcytosine in genomic DNA, gibberellin and protein content with floral bud differentiation of Brassica parachinensis Barley. J Zhejiang Agric Univ 28:161–164Google Scholar
  24. Li JH, Li YH, Chen SY, An LZ (2010) Involvement of brassinosteroid signals in the floral-induction network of Arabidopsis. J Exp Bot 61:4221–4230PubMedGoogle Scholar
  25. Liao CJ, Lai Z, Lee S, Yun DJ, Mengiste T (2016) Arabidopsis HOOKLESS1 regulates responses to pathogens and abscisic acid through interaction with MED18 and acetylation of WRKY33 and ABI5 chromatin. Plant Cell 28:1662–1681PubMedPubMedCentralGoogle Scholar
  26. Lin SI, Wang JG, Poon SY, Su CL, Wang SS, Chiou TJ (2005) Differential regulation of FLOWERING LOCUS C expression by vernalization in cabbage and Arabidopsis. Plant Physiol 137:1037–1048PubMedPubMedCentralGoogle Scholar
  27. Manzano S, Martínez C, Megías Z, Gómez P, Garrido D, Jamilena M (2011) The role of ethylene and brassinosteroids in the control of sex expression and flower development in Cucurbita pepo. Plant Growth Regul 65:213–221Google Scholar
  28. Michaels SD, Amasino RM (2000) Memories of winter: vernalization and the competence to flower. Plant Cell Environ 23:1145–1153Google Scholar
  29. Mishra P, Panigrahi KC (2015) GIGANTEA-an emerging story. Front Plant Sci 6:8PubMedPubMedCentralGoogle Scholar
  30. Mutasa-Göttgens E, Hedden P (2009) Gibberellin as a factor in floral regulatory networks. J Exp Bot 60:1979–1989PubMedGoogle Scholar
  31. Mutasa-Göttgens E, Qi A, Mathews A, Thomas S, Phillips A, Hedden P (2009) Modification of gibberellin signalling (metabolism & signal transduction in sugar beet: analysis of potential targets for crop improvement. Transgenic Res 18:301–308PubMedGoogle Scholar
  32. Norikoshi R, Shibata T, Ichimura K (2016) Cell division and expansion in petals during flower development and opening in Eustoma grandiflorum. Hortic J 85:154–160Google Scholar
  33. Papadopoulou E, Grumet R (2005) Brassinosteriod-induced femaleness in cucumber and relationship to ethylene production. Hortscience 40:1763–1767Google Scholar
  34. Peterson R, Slovin JP, Chen C (2010) A simplified method for differential staining of aborted and non-aborted pollen grains. Int J Plant Biol 1:e13Google Scholar
  35. Qayyum A (2011) Water stress causes differential effects on germination indices, total soluble sugar and proline content in wheat (Triticum aestivum L.) genotypes. Afr J Biotechnol 10:14038–14045Google Scholar
  36. Reinoso H, Luna V, Dauria C, Pharis RP, Bottini R (2002) Dormancy in peach (Prunus persica) flower buds. VI. Effects of gibberellins and an acylcyclohexanedione (trinexapac-ethyl) on bud morphogenesis in field experiments with orchard trees and on cuttings. Can J Bot 80:664–674Google Scholar
  37. Shillo R (1976) Control of flower initiation and development of statice (Limonium sinuatum) by temperature and daylength. Acta Hortic 64:197–203Google Scholar
  38. Shu K, Chen Q, Wu Y, Liu R, Zhang H, Wang S, Tang S, Yang W, Xie Q (2016) ABSCISIC ACID-INSENSITIVE 4 negatively regulates flowering through directly promoting Arabidopsis FLOWERING LOCUS C transcription. J Exp Bot 67:195–205PubMedGoogle Scholar
  39. Su WR, Huang KL, Shen RS, Chen WS (2002) Abscisic acid affects floral initiation in Polianthes tuberosa. J Plant Physiol 159:557–559Google Scholar
  40. Szekeres M, Németh K, Konczkálmán Z, Mathur J, Kauschmann A, Altmann T, Rédei GP, Nagy F, Schell J, Koncz C (1996) Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell 85:171–182PubMedGoogle Scholar
  41. Trevaskis B, Hemming MN, Dennis ES, Peacock WJ (2007) The molecular basis of vernalization-induced flowering in cereals. Trends Plant Sci 12:352–357PubMedGoogle Scholar
  42. Turck F, Fornara F, Coupland G (2008) Regulation and identity of florigen: FLOWERING LOCUS T moves center stage. Annu Rev Plant Biol 59:573–594PubMedGoogle Scholar
  43. Vaz AP, de Cássia LR, Kerbauy GB (2004) Photoperiod and temperature effects on in vitro growth and flowering of P. pusilla, an epiphytic orchid. Plant Physiol Biochem 42:411–415PubMedGoogle Scholar
  44. Wilfret GJ, Raulston JC (1975) Acceleration of flowering of statice (Limonium sinuatum Mill.) by gibberellic acid (GA3). HortScienceGoogle Scholar
  45. Wilmowicz E, Kesy J, Kopcewicz J (2008) Ethylene and ABA interactions in the regulation of flower induction in Pharbitis nil. J Plant Physiol 165:1917–1928PubMedGoogle Scholar
  46. Yang YM, Xu CN, Wang BM, Jia JZ (2001) Effects of plant growth regulators on secondary wall thickening of cotton fibres. Plant Growth Regul 35:233–237Google Scholar
  47. Ye QQ, Zhu WJ, Li L, Zhang SS, Yin YH, Hong M, Wang XL (2010) Brassinosteroids control male fertility by regulating the expression of key genes involved in Arabidopsis anther and pollen development. Proc Natl Acad Sci 107:6100–6105PubMedGoogle Scholar
  48. Yuan F, Chen M, Yang JC, Leng BY, Wang BS (2014) A system for the transformation and regeneration of the recretohalophyte Limonium bicolor. In Vitro Cell Dev Plant 50:610–617Google Scholar
  49. Yuan F, Chen M, Yang J, Song J, Wang BS (2015a) The optimal dosage of 60Co gamma irradiation for obtaining salt gland mutants of exorecretohalophyte Limonium bicolor (Buntze) O. Kuntze. Pak J Bot 47:71–76Google Scholar
  50. Yuan F, Lyu MJA, Leng BY, Zheng GY, Feng ZT, Li PH, Zhu XG, Wang BS (2015b) Comparative transcriptome analysis of developmental stages of the Limonium bicolor leaf generates insights into salt gland differentiation. Plant Cell Environ 38:1637–1657PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Shandong Provincial Key Laboratory of Plant Stress, College of Life SciencesShandong Normal UniversityJinanPeople’s Republic of China

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