Application of Uniconazole Improves Photosynthetic Efficiency of Maize by Enhancing the Antioxidant Defense Mechanism and Delaying Leaf Senescence in Semiarid Regions
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The degradation of photosynthetic pigments leads to leaf senescence and thus grain yield losses. We determined whether the application of uniconazole to maize in semiarid regions could reduce the degradation of photosynthetic pigments by enhancing the antioxidant defense system. We conducted a field study in the summers of 2015 and 2016 where seeds were soaked in uniconazole at concentrations of 0 (CK1), 25 (S1), 50 (S2), and 75 (S3) mg kg−1 and foliar sprayed at concentrations of 0 (CK2), 25 (F1), 50 (F2), and 75 (F3) mg L−1 at the eight-leaf stage. The application of uniconazole significantly improves the chlorophyll content, soluble protein content, and net photosynthetic rate, where the maximum values were obtained with the S1 and F1 treatments. Uniconazole significantly improved the activities of antioxidant enzymes comprising superoxide dismutase, peroxidase, and catalase, but reduced that of malondialdehyde (MDA) and the accumulation of reactive oxygen species (ROS) during the leaf senescence process. Treatments S1 and F1 had higher antioxidant enzyme activities but reduced MDA, superoxide radical, and hydrogen peroxide contents. Uniconazole significantly reduced leaf senescence in leaves in the bottom layer, while also increasing the middle layer leaf area and decreasing the top layer leaf area. The degradation of photosynthetic pigments was reduced by uniconazole because the enhanced antioxidant activities of enzymes protected plants from harmful ROS. Uniconazole significantly improved the photosynthetic traits, antioxidant defense system, and grain yield in maize in semiarid regions, where the most effective treatment was S1.
KeywordsAntioxidant defense system Leaf senescence Maize production Photosynthetic trait Semiarid region Uniconazole
The authors extend their sincere thanks to the editors of this journal and the anonymous reviewers for their valuable comments and suggestions that have significantly improved the manuscript. We are also grateful to Ding Ruixia, Nie Junfeng, and Yang Baoping for help during experimental period.
QH, LT, and TC conceived and designed the research. IA performed research. MK, WX, SA, and SA contributed in the field experiments. IA wrote the manuscript. BB helped in English revision. WS helped in revision of the manuscript.
This study was supported by funding from High Technology Research and Development Program of China (863 Program, No.2013AA102902), the National Natural Science Foundation of China (No. 31601256), the special fund for Agro-scientific Research in the Public Interest (201303104), the 111 Project of Chinese Education Ministry (B12007).
Compliance with Ethical Standards
Conflict of interest
The authors have no conflict of interest.
- Aly A, Latif H (2011) Differential effects of paclobutrazol on water stress alleviation through electrolyte leakage, phytohormones, reduced glutathione and lipid peroxidation in some wheat genotypes (Triticum aestivum L.) grown in-vitro. Rom Biotechnol Lett 16:6710–6721Google Scholar
- Bhattacharjee S (2014) Membrane lipid peroxidation and its conflict of interest: the two faces of oxidative stress. Curr Sci 107:1811–1823Google Scholar
- Catherine MS (2010) Gene expression during leaf senescence. New Phytol 126:419–448Google Scholar
- Dai HP, Zhang PP, Lu C, Jia GL, Song H, Ren XM, Chen J, Wei AZ, Feng BL, Zhang SQ (2011) Leaf senescence and reactive oxygen species metabolism of broomcorn millet (Panicum miliaceum L.) under drought condition. Aust J Crop Sci 5:1655–1660Google Scholar
- Dat J, Vandenabeele S, Vranova E, Van Montagu M, Inze D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 581:320–324Google Scholar
- Fang X, Liu X, Zhang Y, Huang K, Zhang Y, Li Y, Nie J, She H, Ruan R, Yuan X, Yi Z (2018) Effects of uniconazole or gibberellic acid application on the lignin metabolism in relation to lodging resistance of culm in common buckwheat (Fagopyrum esculentum M.). J Agron Crop Sci 00:1–10Google Scholar
- Fletcher RA, Gilley A, Davis TD, Sankhla N (2000) Triazole as plant growth regulators and stress protectants. Hort Rev 24:55–138Google Scholar
- Han LP, Wang XL, Guo XQ, Rao MS, Steinberger Y, Xu C, Xie GH (2011) Effects of plant growth regulators on growth, yield and lodging of sweet sorghum. Res Crops 12:372–382Google Scholar
- Hussein MM, Bakheta MA, Zaki SNS (2014) Influence of uniconazole on growth characters, photosynthetic pigments, total carbohydrates and total soluble sugars of Hordium vulgare L plants grown under salinity stress. Int J Sci Res 3:2208–2213Google Scholar
- Kamran M, Cui W, Ahmad I, Meng XP, Zhang X, Su W, Chen J, Ahmad S, Fahad S, Han QF, Tiening L (2018a) Effect of paclobutrazol, a potential growth regulator on stalk mechanical strength, lignin accumulation and its relation with lodging resistance of maize. Plant Growth Regul 84:317–332CrossRefGoogle Scholar
- Kamran M, Su W, Ahmad I, Xiangping M, Wenwen C, Xudong Z, Siwei M, Khan A, Qingfang H, Tiening L (2018b) Application of paclobutrazol affect maize grain yield by regulating root morphological and physiological characteristics under a semi-arid region. Sci Rep 8:4818CrossRefPubMedPubMedCentralGoogle Scholar
- Kim HY, Choi BJ, Sang CK (1994) Effects of uniconazole on the drought resistance of Pilea cadierei. II. Physiological changes and drought resistance. J Korean Soc Hort Sci 35:493–498Google Scholar
- Ku JH, Drizek DT, Mirecki RM (1996) Alleviation of sulfur dioxide injury in snap bean plants by uniconazole. J Korean Soc Hort Sci 37:767–772Google Scholar
- Lin ZF, Li SX, Lin GZ, Guo GZ (1988) Relationship between cumulation of H2O2 and membrane lipid peroxidation in senescent leaf and chloroplast. Acta Photophysiol Sin 14:16–22Google Scholar
- Liu Y, Fang Y, Huang M, Jin Y, Sun J, Tao X, Zhang G, He K, Zhao Y, Zhao H (2015a) Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) I: transcriptome analysis of the effects of uniconazole on chlorophyll and endogenous hormone biosynthesis. Biotechnol Biofuels 8:57CrossRefPubMedPubMedCentralGoogle Scholar
- Liu Y, Fang Y, Huang MJ, Jin YL, Sun JL, Tao X, Zhang GH, He KZ, Zhao Y, Zhao H (2015b) Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) II: transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormone crosstalk. Biotechnol Biofuels 8:57CrossRefPubMedPubMedCentralGoogle Scholar
- Lu W, Xu XM, Zhang RX, Dai XB (2004) Effect of adding acetic acid on improvement of determination of superoxide anion content in plants. J N Norm Univ 27:82–84Google Scholar
- Nooden L (1988) Whole plant senescence. In: Nooden LD, Leopold AC (eds) Senescence and aging in plants. Academic Press, San Diego, pp 391–439Google Scholar
- Nouriyani H, Majidi E, Seyyednejad SM, Siadat SA, Naderi A (2012) Effect of paclobutrazol under different levels of nitrogen on some physiological traits of two wheat cultivars (Triticum aestivum L.). World Appl Sci J 16:1–6Google Scholar
- Percival C, Albalushi M (2007) Paclobutrazol-induced drought tolerance in containerized english and evergreen oak. Arboricult Urban For 33:397–409Google Scholar
- Sang HL, Ahsan N, Lee KW, Kim DH, Lee DG, Kwak SS, Kwon SY, Kim TH, Lee BH (2007) Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J Plant Physiol 64:1626–1638Google Scholar
- Scandalios JG (1997) Molecular genetics of SOD in plants. In: Scandalios JG (ed) Oxidative stress and the molecular biology of antioxidant defense. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 527–568Google Scholar
- Somasundaram R, Jaleel CA, Sindhu SA, Azooz MM, Panneerselvam R (2009) Role of Paclobutrazol and ABA in drought stress amelioration in Sesamum indicum L. Glob J Mol Sci 4:56–62Google Scholar
- Wang AG, Luo GH (1990) Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol Commun 84:2895–2898Google Scholar
- Wang XC, Yang WY, Chen G, Qian-Liang LI, Wang XB (2009) Effects of uniconazole on leaf senescence and yield of maize sprayed at late growth stage. J Maize Sci 17:86–88Google Scholar
- Wang YC, Gu WR, Ye LF, Sun Y, Jie LL, Zhang H, Li J, Wei S (2015b) Physiological mechansim of delaying leaf senescence in maize treated with compound mixtures of DCPTA and CCC. J Northeast Agric Univ 22:1–15Google Scholar
- Zhang ZL (2001) Experimental guide of plant physiology. Higher Education Press, BeijingGoogle Scholar
- Zhang J, Cao XL, Yong TW, Yang WY (2012) Seed treatment with uniconazole powder induced drought tolerance of soybean in relation to changes in photosynthesis and chlorophyll fluorescence. Res Crops 13:147–154Google Scholar