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Kinetin Alleviates UV-B-Induced Damage in Solanum lycopersicum: Implications of Phenolics and Antioxidants

  • Madhulika Singh
  • Gausiya Bashri
  • Sheo Mohan PrasadEmail author
  • Vijay Pratap Singh
Article
  • 42 Downloads

Abstract

Cytokinins, a class of plant growth regulators, are known to regulate several plant developmental processes and may play a significant role in protecting them against abiotic stresses. Thus, this study was focused on the effect of kinetin (KN, 10 µM) treatment on phenylpropanoid pathway: PAL activity, phenolics, flavonoids, and anthocyanins contents, and oxidative stress biomarkers, antioxidant potential, and growth in tomato seedlings exposed to UV-B (UV-B1, ambient + 1.2 kJ m−2 day−1 and UV-B2, ambient + 2.4 kJ m−2 day−1) stress. UV-B exposure to KN untreated seedlings caused substantial increase in reactive oxygen species contents: superoxide radical and hydrogen peroxide (H2O2), and oxidative damage biomarkers: lipid peroxidation and electrolyte leakage in a dose-dependent manner, despite increased antioxidant potential: super oxide dismutase (SOD), peroxidase (POD), catalase (CAT) and glutathione-S-transferase (GST) activity, and radical scavenging capacity (DPPH activity), PAL activity, total phenolics, flavonoids and anthocyanins contents. The seedlings pre-treated with KN and subsequently exposed to UV-B exhibited substantial increase (i) in antioxidant potential: SOD, POD, CAT and GST activity, and DPPH activity and (ii) PAL activity which led to a further rise in total phenolics, UV-B screening pigments (flavonoids) and anthocyanins and hence, significant lowering in oxidative stress biomarkers was noticed. The overall results show that KN pre-treatment significantly alleviated UV-B-induced damaging effects in tomato seedlings by regulating phenolics and antioxidants.

Keywords

Antioxidants Kinetin Solanum lycopersicum L. Oxidative stress UV-B radiation UV-B screening pigments 

Notes

Acknowledgements

The authors are grateful to the University Grants Commission, New Delhi for providing financial support to Madhulika Singh (UGC-AU-research fellowship scheme) and also to Indian Council of Medical and Research, New Delhi, India for providing financial support to Gausiya Bashri as Senior Research Fellow.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Madhulika Singh
    • 1
  • Gausiya Bashri
    • 1
  • Sheo Mohan Prasad
    • 1
    Email author
  • Vijay Pratap Singh
    • 2
  1. 1.Ranjan Plant Physiology and Biochemistry Laboratory, Department of BotanyUniversity of AllahabadAllahabadIndia
  2. 2.Department of Botany, C.M.P. Degree CollegeA Constituent PG College of University of AllahabadAllahabadIndia

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