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Journal of Plant Growth Regulation

, Volume 38, Issue 2, pp 683–700 | Cite as

Salt Stress Induces Increase in Starch Accumulation in Duckweed (Lemna aequinoctialis, Lemnaceae): Biochemical and Physiological Aspects

  • Marciana Bizerra de MoraisEmail author
  • Adauto Gomes Barbosa-Neto
  • Lilia Willadino
  • Cláudia Ulisses
  • Tercilio Calsa Junior
Article

Abstract

In this study, antioxidant processes were searched for in macrophyte duckweed to investigate tolerance mechanisms in this species against oxidative damage caused by salinity stress. Biochemical and histological analyses were performed on four Lemna aequinoctialis clones grown in Schenk-Hildebrandt medium, 0.5 × SH, supplemented with 1% sucrose liquid medium containing or not containing NaCl in different NaCl concentrations (0, 25 and 50 mM). For most clones, the salt stress effects caused growth inhibition and antioxidant responses at 50 mM NaCl. Also, starch and reducing sugar accumulations were increased with salt, whereas the photosynthetic pigment content was reduced in clone L. aequinoctialis 5569. The plant growth inhibition reflects the oxidative stress shown by the significant increase in malondialdehyde (MDA) and hydrogen peroxide (H2O2) content. In the L. aequinoctialis 5568 clone, with the highest MDA levels, no antioxidant enzymatic activity was observed. The L. aequinoctialis 5570 clone presented higher ascorbate peroxidase and catalase activities in parallel, indicating that the efficiency of the defence mechanism relies on synchrony between such enzyme activities toward successive elimination of reactive oxygen species and resulting in the assurance of some level of protection of the metabolism from oxidative damage. Considering the moderate salt stress (25 mM), the maintenance of MDA content and small growth inhibition associated with the high starch production suggested the acclimation efficiency of L. aequinoctialis 5570 and 5567 clones, indicating that they may be suitable for cultivation under moderate saline conditions, serving as biofuel feedstock. In addition, this study demonstrates great intraspecific phenotypic plasticity of duckweed, L. aequinoctialis, from closely related clones.

Keywords

Antioxidant defence Abiotic stress Oxidative stress 

Notes

Acknowledgements

The authors gratefully acknowledge the Universidade Federal de Pernambuco for support and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for a scholarship granted to the first author.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. 1.Programa de Pós-graduação em Ciências Naturais (PPGCN)Universidade do Estado do Rio Grande do Norte (UERN)MossoróBrazil
  2. 2.Departamento de GenéticaUniversidade Federal de PernambucoRecifeBrazil
  3. 3.Departamento de BiologiaUniversidade Federal Rural de PernambucoRecifeBrazil

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