Advertisement

Journal of Plant Growth Regulation

, Volume 38, Issue 2, pp 631–643 | Cite as

Genome-wide Identification, Phylogenetic Analysis, and Expression Profiling of CONSTANS-like (COL) Genes in Vitis vinifera

  • Ling Wang
  • Jingyi Xue
  • Weina Dai
  • Yujin Tang
  • Peijie Gong
  • Yuejin Wang
  • Chaohong ZhangEmail author
Article
  • 170 Downloads

Abstract

The CONSTANS (CO) gene plays an important role in the flowering of plants. However, the other precise roles of the CO gene are poorly understood. We carried out a genomic census and analysis of expression patterns for CONSTANS-like genes in Vitis vinifera (VviCOLs) to reveal the molecular characteristics of VviCOLs. Twelve VviCOLs were identified and 11 of their full-length complementary DNAs were cloned. Multiple sequence alignment suggested the VviCOLs contained B-box and CCT conserved domains. We further classified the VviCOLs into three groups according to the variability of the second B-box domain. Synteny analysis showed that eight orthologous gene pairs were identified between grapevine and Arabidopsis, suggesting that eight pairs may descend from a common evolutionary ancestor. Tissue expression analysis of COL genes in cv. Pinot Noir showed VviCOL11a and VviCOL11b were specifically expressed in flower bud, whereas VviCOL16b was only expressed in leaves. Ten VviCOLs were expressed in the developing ovule and six of them showed higher expression in the ovule of cv. Thompson Seedless than that of cv. Pinot Noir, indicating that VviCOLs were involved in the process of seed development or ovule abortion. Furthermore, nine of twelve VviCOLs were expressed in cv. Pinot Noir leaves and all of these nine genes had a response to exogenous hormone application. In summary, our findings provide a new insight into the further studies of VviCOLs, especially in terms of seed development and hormone response.

Keywords

Vitis vinifera CONSTANS-like Seed development Hormone stress Expression profiles 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No.31372023) and Modern Agro-industry Technology Research System (No. CARS-30-yz-7).

Author Contributions

CHZ conceived and designed the experiments. LW and JYX performed the experiments. LW, WND, YJT, PJG, and CHZ analyzed the data and manuscript modification. CHZ and YJW contributed reagents/materials/analysis tools. LW and CHZ contributed to the writing of the manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

344_2018_9878_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 24 KB)

References

  1. Almada R, Cabrera N, Casaretto JA, Ruiz-Lara S, Villanueva EG (2009) VvCO and VvCOL1, two CONSTANS homologous genes, are regulated during flower induction and dormancy in grapevine buds. Plant Cell Rep 28:1193–1203CrossRefGoogle Scholar
  2. Burland TG (2009) DNASTAR’s Lasergene sequence analysis software. Methods Mol Biol 132:71–91Google Scholar
  3. Cai D, Liu H, Sang N, Huang X (2017) Identification and characterization of CONSTANS-like (COL) gene family in upland cotton (Gossypium hirsutum L.). PLoS ONE 12:e0179038CrossRefGoogle Scholar
  4. Campoli C, Drosse B, Searle I, Coupland G, von Korff M (2012) Functional characterisation of HvCO1, the barley (Hordeum vulgare) flowering time ortholog of CONSTANS. Plant J 69:868–880CrossRefGoogle Scholar
  5. Chaurasia AK, Patil HB, Azeez A, Subramaniam VR, Krishna B, Sane AP, Sane PV (2016) Molecular characterization of CONSTANS-Like (COL) genes in banana (Musa acuminata L. AAA Group, cv. Grand Nain). Physiol Mol Biol Plants 22:1–15CrossRefGoogle Scholar
  6. Chen J, Chen JY, Wang JN, Kuang JF, Shan W, Lu WJ (2012) Molecular characterization and expression profiles of MaCOL1, a CONSTANS-like gene in banana fruit. Gene 496:110–117CrossRefGoogle Scholar
  7. Cheng XF, Wang ZY (2005) Overexpression of COL9, a CONSTANS-LIKE gene, delays flowering by reducing expression of CO and FT in Arabidopsis thaliana. Plant J 43:758–768CrossRefGoogle Scholar
  8. Cockram J, Thiel T, Steuernagel B, Stein N, Taudien S, Bailey PC, O’Sullivan DM (2012) Genome dynamics explain the evolution of flowering time CCT domain gene families in the poaceae. PLoS ONE 7:e45307CrossRefGoogle Scholar
  9. Crooks GE, Hon G, Chandonia JM, Brenner SE (2004) WebLogo: a sequence logo generator. Genome Res 14:1188–1190CrossRefGoogle Scholar
  10. Datta S, Hettiarachchi G, Deng XW, Holm M (2006) Arabidopsis CONSTANS-LIKE3 is a positive regulator of red light signaling and root growth. Plant Cell 18:70–84CrossRefGoogle Scholar
  11. Deng XD, Fan XZ, Li P, Fei XW (2015) A photoperiod-regulating gene CONSTANS is correlated to lipid biosynthesis in Chlamydomonas reinhardtii. Biomed Res Int 715020Google Scholar
  12. Fu J, Yang L, Dai S (2015) Identification and characterization of the CONSTANS-like gene family in the short-day plant Chrysanthemum lavandulifolium. Mol Genet Genomics 290:1039–1054CrossRefGoogle Scholar
  13. Gasteiger E, Gattiker A, Hoogland C, Ivanyi I, Appel RD, Bairoch A (2003) ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res 31:3784–3788CrossRefGoogle Scholar
  14. Gonzalez-Schain ND, Suarez-Lopez P (2008) CONSTANS delays flowering and affects tuber yield in potato. Biol Plantarum 52:251–258CrossRefGoogle Scholar
  15. Gonzalez-Schain ND, Diaz-Mendoza M, Zurczak M, Suarez-Lopez P (2012) Potato CONSTANS is involved in photoperiodic tuberization in a graft-transmissible manner. Plant J 70:678–690CrossRefGoogle Scholar
  16. Griffiths S, Dunford RP, Coupland G, Laurie DA (2003) The evolution of CONSTANS-like gene families in barley, rice, and Arabidopsis. Plant Physiol 131:1855–1867CrossRefGoogle Scholar
  17. Grimplet J, Adamblondon AF, Bert PF, Bitz O, Cantu D, Davies C, Delrot S, Pezzotti M, Rombauts S, Cramer GR (2014) The grapevine gene nomenclature system. BMC Genomics 15:1077CrossRefGoogle Scholar
  18. Hassidim M, Harir Y, Yakir E, Kron I, Green RM (2009) Over-expression of CONSTANS-LIKE 5 can induce flowering in short-day grown Arabidopsis. Planta 230:481–491CrossRefGoogle Scholar
  19. Holefors A, Opseth L, Rosnes AKR, Ripel L, Snipen L, Fossdal CG, Olsen JE (2009) Identification of PaCOL1 and PaCOL2 two CONSTANS-like genes showing decreased transcript levels preceding short day induced growth cessation in Norway spruce. Plant Physiol Bioch 47:105–115CrossRefGoogle Scholar
  20. Hsu CY, Adams JP, No K, Liang HY, Meilan R, Pechanova O, Barakat A, Carlson JE, Page GP, Yuceer C (2012) Overexpression of constans homologs CO1 and CO2 fails to alter normal reproductive onset and fall bud set in woody perennial poplar. PloS One 7:e45448CrossRefGoogle Scholar
  21. Hu B, Jin J, Guo AY, Zhang H, Luo J, Gao G (2014) GSDS 2.0: an upgraded gene feature visualization server. Bioinformatics 31:1296–1297CrossRefGoogle Scholar
  22. Hu Y, Han YT, Zhang K, Zhao FL, Li YJ, Zheng Y, Wang YJ, Wen YQ (2016) Identification and expression analysis of heat shock transcription factors in the wild Chinese grapevine (Vitis pseudoreticulata). Plant Physiol Bioch 99:1–10CrossRefGoogle Scholar
  23. Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, Casagrande A, Choisne N, Aubourg S, Vitulo N, Jubin C, Vezzi A, Legeai F, Hugueney P, Dasilva C, Horner D, Mica E, Jublot D, Poulain J, Bruyere C, Billault A, Segurens B, Gouyvenoux M, Ugarte E, Cattonaro F, Anthouard V, Vico V, Del Fabbro C, Alaux M, Di Gaspero G, Dumas V, Felice N, Paillard S, Juman I, Moroldo M, Scalabrin S, Canaguier A, Le Clainche I, Malacrida G, Durand E, Pesole G, Laucou V, Chatelet P, Merdinoglu D, Delledonne M, Pezzotti M, Lecharny A, Scarpelli C, Artiguenave F, Pe ME, Valle G, Morgante M, Caboche M, Adam-Blondon AF, Weissenbach J, Quetier F, Wincker P (2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449:463–467CrossRefGoogle Scholar
  24. Kikuchi R, Kawahigashi H, Oshima M, Ando T, Handa H (2012) The differential expression of HvCO9, a member of the CONSTANS-like gene family, contributes to the control of flowering under short-day conditions in barley. J Exp Bot 63:773–784CrossRefGoogle Scholar
  25. Kim SJ, Moon J, Lee I, Maeng J, Kim SR (2003) Molecular cloning and expression analysis of a CONSTANS homologue, PnCOL1, from Pharbitis nil. J Exp Bot 54:1879–1887CrossRefGoogle Scholar
  26. Kim SK, Yun CH, Lee JH, Jang YH, Park HY, Kim JK (2008) OsCO3, a CONSTANS-LIKE gene, controls flowering by negatively regulating the expression of FT-like genes under SD conditions in rice. Planta 228:355–365CrossRefGoogle Scholar
  27. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948CrossRefGoogle Scholar
  28. Ledger S, Strayer C, Ashton F, Kay SA, Putterill J (2001) Analysis of the function of two circadian-regulated CONSTANS-LIKE genes. Plant J 26:14–22CrossRefGoogle Scholar
  29. Li H, Xu Y, Xiao Y, Zhu Z, Xie X, Zhao H, Wang Y (2010) Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata. Planta 232:1325–1337CrossRefGoogle Scholar
  30. Lind-Riehl JF, Sullivan AR, Gailing O (2014) Evidence for selection on a CONSTANS-like gene between two red oak species. Ann Bot 113:967–975CrossRefGoogle Scholar
  31. Liu L, Ma J, Han Y, Chen X, Fu YF (2011) The isolation and analysis of a soybean CO homologue GmCOL10. Russ J Plant Physiol 58:330–336CrossRefGoogle Scholar
  32. Liu H, Dong S, Sun D, Liu W, Gu F, Liu Y, Guo T, Wang H, Wang J, Chen Z (2016) CONSTANS-like 9 (OsCOL9) interacts with receptor for activated C-kinase 1(OsRACK1) to regulate blast resistance through salicylic acid and ethylene signaling pathways. PLoS ONE 11:eo166249Google Scholar
  33. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods 25:402–408CrossRefGoogle Scholar
  34. Marchlerbauer A, Anderson JB, Deweesescott C (2003) CDD: a curated Entrez database of conserved domain alignments. Nucleic Acids Res 31:383–387CrossRefGoogle Scholar
  35. Min JH, Chung JS, Lee KH, Kim CS (2015) The CONSTANS-like 4 transcription factor, AtCOL4, positively regulates abiotic stress tolerance through an abscisic acid-dependent manner in Arabidopsis. J Integr Plant Biol 57:313–324CrossRefGoogle Scholar
  36. Putterill J, Robson F, Lee K, Simon R, Coupland G (1995) The CONSTANS gene of Arabidopsis promotes flowering and encodes a protein showing similarities to zinc-finger transcription factors. Cell 80:847–857CrossRefGoogle Scholar
  37. Robson F, Costa MMR, Hepworth SR, Vizir I, Pineiro M, Reeves PH, Putterill J, Coupland G (2001) Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants. Plant J 28:619–631CrossRefGoogle Scholar
  38. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evolut 4:406Google Scholar
  39. Schultz J, Milpetz F, Bork P, Ponting CP (1998) SMART, a simple modular architecture research tool: Identification of signaling domains. Proc Natl Acad Sci USA 95:5857–5864CrossRefGoogle Scholar
  40. Sun B, Cao HN, He W, Gong YM, Zong CL (2009) Study on embryo abortion stage of pseudoparthenocarpy seedless grape. J Agric Sci Yanbian Univ 31(3):159–164Google Scholar
  41. Takase T, Kakikubo Y, Nakasone A, Nishiyama Y, Yasuhara M, Tokioka-Ono Y, Kiyosue T (2011) Characterization and transgenic study of CONSTANS-LIKE8 (COL8) gene in Arabidopsis thaliana: expression of 35S:COL8 delays flowering under long-day conditions. Plant Biotechnol 28:439–446CrossRefGoogle Scholar
  42. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefGoogle Scholar
  43. Tang H, Wang X, Bowers JE, Ming R, Alam M, Paterson AH (2008) Unraveling ancient hexaploidy through multiply-aligned angiosperm gene maps. Genome Res 18:1944–1954CrossRefGoogle Scholar
  44. Valverde F, Mouradov A, Soppe W, Ravenscroft D, Samach A, Coupland G (2004) Photoreceptor regulation of CONSTANS protein in photoperiodic flowering. Science 303:1003–1006CrossRefGoogle Scholar
  45. Wang YJ, Jiang SP, Liu XN, Zhang JX (2007) Mechanism of embryo abortion in stenopermocarpic seedless grape. Acta BotBoreal OccidentSin 27(10):1987–1993Google Scholar
  46. Wang HG, Zhang ZL, Li HY, Zhao XY, Liu XM, Ortiz M, Lin CT, Liu B (2013) CONSTANS-LIKE 7 regulates branching and shade avoidance response in Arabidopsis. J Exp Bot 64:1017–1024CrossRefGoogle Scholar
  47. Wenkel S, Turck F, Singer K, Gissot L, Le Gourrierec J, Samach A, Coupland G (2006) CONSTANS and the CCAAT box binding complex share a functionally important domain and interact to regulate flowering of Arabidopsis. Plant Cell 18:2971–2984CrossRefGoogle Scholar
  48. Wong ACS, Hecht VFG, Picard K, Diwadkar P, Laurie RE, Wen J, Mysore K, Macknight RC, Weller JL (2014) Isolation and functional analysis of CONSTANS-LIKE genes suggests that a central role for CONSTANS in flowering time control is not evolutionarily conserved in Medicago truncatula. Front Plant Sci 5:486CrossRefGoogle Scholar
  49. Yang SS, Weers BD, Morishige DT, Mullet JE (2014) CONSTANS is a photoperiod regulated activator of flowering in sorghum. BMC Plant Biol 14:148CrossRefGoogle Scholar
  50. Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T (2000) Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12:2473–2483CrossRefGoogle Scholar
  51. Zhang JX, Wu KL, Tian LN, Zeng SJ, Duan J (2011) Cloning and characterization of a novel CONSTANS-like gene from Phalaenopsis hybrida. Acta Physiol Plant 33:409–417CrossRefGoogle Scholar
  52. Zhang C, Gong P, Wei R, Li S, Zhang X, Yu Y, Wang Y (2013) The metacaspase gene family of Vitis vinifera L.: characterization and differential expression during ovule abortion in stenospermocarpic seedless grapes. Gene 528:267–276CrossRefGoogle Scholar
  53. Zhang R, Ding J, Liu C, Cai C, Zhou B, Zhang T, Guo W (2015a) Molecular evolution and phylogenetic analysis of eight COL superfamily genes in group I related to photoperiodic regulation of flowering time in wild and domesticated cotton (Gossypium) species. PLoS ONE 10:e0118669CrossRefGoogle Scholar
  54. Zobell O, Coupland G, Reiss B (2005) The family of CONSTANS-like genes in Physcomitrella patens. Plant Biol 7:266–275CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ling Wang
    • 1
    • 2
  • Jingyi Xue
    • 1
    • 2
  • Weina Dai
    • 1
    • 2
  • Yujin Tang
    • 1
    • 2
  • Peijie Gong
    • 1
    • 2
  • Yuejin Wang
    • 1
    • 2
  • Chaohong Zhang
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Crop Stress Biology for Arid Areas, College of HorticultureNorthwest A&F UniversityYanglingChina
  2. 2.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region)Ministry of Agriculture, People’s Republic of ChinaYanglingChina

Personalised recommendations