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Journal of Plant Growth Regulation

, Volume 38, Issue 2, pp 574–585 | Cite as

CaMYC, A Novel Transcription Factor, Regulates Anthocyanin Biosynthesis in Color-leaved Pepper (Capsicum annuum L.)

  • Bo-Ya Lu
  • Guo-Xin Cheng
  • Zhen Zhang
  • Jian-Tian Sun
  • Muhammad Ali
  • Qing-Li Jia
  • Luo De-Xu 
  • Zhen-Hui GongEmail author
  • Da-Wei LiEmail author
Article
  • 203 Downloads

Abstract

Anthocyanins are secondary metabolites derived from the general phenylpropanoid pathway and are widespread throughout the plant kingdom. Anthocyanin accumulation is regulated by transcription factors. However, little is known about the role of CaMYC from the bHLH family in pepper anthocyanin synthesis. In this study, a purple-leaved pepper (Capsicum annuum L.) was selected and subjected to light and cold stress at the seedling stage. The result showed that anthocyanin content in purple leaves was higher than in green leaves, which was related to anthocyanins synthesis. CaMYC is an important factor and may be responsible for anthocyanin synthesis. Light and cold stress can result in strong anthocyanin accumulation. CaMYC plays a key role in increasing structural gene expression, which regulates anthocyanin synthesis by the combination of CaMYB and CaWD40. Further analysis showed that CaMYC-silencing resulted in low expression of structural genes in the anthocyanin synthesis pathway, suggesting that CaMYC has a positive role in pepper anthocyanin metabolism. Our study will provide an insight for anthocyanin synthesis in pepper.

Keywords

Pepper Anthocyanin CaMYC Cold stress Light 

Notes

Acknowledgements

This work was supported through funding from the National Natural Science Foundation of China (No. U1603102), the National Key R&D Program of China (No. 2016YFD0101900), and the Independent Innovation Fund Project of Agricultural Science and Technology in Jiangsu [No.CX (17) 3040].

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

344_2018_9871_MOESM1_ESM.pdf (343 kb)
Supplementary material 1 (PDF 342 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Bo-Ya Lu
    • 1
  • Guo-Xin Cheng
    • 1
  • Zhen Zhang
    • 1
  • Jian-Tian Sun
    • 1
  • Muhammad Ali
    • 1
  • Qing-Li Jia
    • 1
  • Luo De-Xu 
    • 2
  • Zhen-Hui Gong
    • 1
    Email author
  • Da-Wei Li
    • 1
    Email author
  1. 1.College of HorticultureNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Xuhuai Region Huaiyin Institute of Agricultural SciencesHuaianPeople’s Republic of China

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