Role of Exogenous Glutathione in Alleviating Abiotic Stress in Maize (Zea mays L.)
- 150 Downloads
The role of exogenous GSH in improving abiotic stress tolerance in maize was investigated in this study. GSH-treated plants showed significantly higher germination percentage, survival rate, plant biomass, and grain yield per plant than control plants. The possible physiological mechanism underlying the tolerance phenotypes in GSH-treated plants were also analyzed in this study. GSH-treated plants showed reduced oxidative destruction, enhanced water retention, and increased activity of antioxidant enzyme, vacuolar H+-pyrophosphatase (V-H+-PPase), and H+-adenosine triphosphatase (V-H+-ATPase), compared to control plants. In addition, the accumulation of abscisic acid (ABA) and the expression of ABA-responsive genes were upregulated by GSH treatment. These results suggested that GSH played a role in relieving oxidative destruction, maintaining plant water content, and promoting higher ABA levels, which were responsible for GSH-enhanced tolerance to abiotic stresses in maize.
KeywordsMaize Glutathione (GSH) Abiotic stress Yield ABA accumulation
This work was supported by the National Natural Science Foundation of China (31771797, 31401388) and by the Natural Science Foundation of Shandong Province (ZR2017BC099).
Compliance with Ethical Standards
Conflict of interest
The authors have declared that no competing interests exist.
- Baisak R, Rana D, Acharya BB, Kar M (1994) Alterations in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress. Plant Cell Physiol 35:489–495Google Scholar
- Dabbous A, Saad RB, Brini F, Farhat-Khemekhem A, Zorrig W, Abdely C, Hamed KB (2017) Over-expression of a subunit E1 of a vacuolar H+-ATPase gene (Lm VHA-E1) cloned from the halophyte Lobularia maritima improves the tolerance of Arabidopsis thaliana to salt and osmotic stresses. Environ Exp Bot 137:128–141CrossRefGoogle Scholar
- Fan L, Zheng S, Wang X (1997) Antisense suppression of phospholipase Dα retards abscisic acid- and ethylene-promoted senescence of postharvest Arabidopsis leaves. Plant Cell 9:2183–2196Google Scholar
- Kattab H (2007) Role of glutathione and polyadenylic acid on the oxidative defense systems of two different cultivars of canola seedlings grown under saline condition. Aust J Basic Appl Sci 1:323–334Google Scholar
- Maehly AC, Chance B (1954) Catalases and peroxidases, part II. Methods Biochem Anal 1:357–424Google Scholar
- Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
- Taiz L (1992) The plant vacuole. J Exp Biol 172:113–122Google Scholar
- Thompson AJ, Andrews J, Mulholland BJ, Mckee JM, Hilton HW, Horridge JS, Farquhar GD, Smeeton RC, Smillie IR, Black CR (2007) Overproduction of abscisic acid in tomato increases transpiration efficiency and root hydraulic conductivity and influences leaf expansion. Plant Physiol 143:1905–1917CrossRefGoogle Scholar
- Vernoux T, Wilson RC, Seeley KA, Reichheld JP, Muroy S, Brown S, Maughan SC, Cobbett CS, Montagu MV, Inzé D, May MJ, Sung ZR (2000) The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. Plant Cell 12:97–109CrossRefGoogle Scholar
- Virlouvet L, Jacquemot MP, Gerentes D, Corti H, Bouton S, Gilard F, Valot B, Trouverie J, Tcherkez G, Falque M (2011) The ZmASR1 protein influences branched-chain amino acid biosynthesis and maintains kernel yield in maize under water-limited conditions. Plant Physiol 157:917–936CrossRefGoogle Scholar